Synthesis, characterization, and evaluation of curcumin‐loaded endodontic reparative material

Abstract

Curcumin (CUR) is an ancient therapeutic agent with remarkable antimicrobial and antiinflammatory properties. The purpose of the current study was to synthesize and evaluate a curcumin-based reparative endodontic material to reduce infection and inflammation besides the induction of mineralization during the healing of the dentin-pulp complex.Poly-ɛ-caprolactone (PCL)/gelatin (Gel)/CUR scaffold was synthesized and assessed by scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis (TGA). Agar diffusion test was performed against E. coli, A. baumannii, P. aeruginosa, S. aureus, E. faecalis, and S. mutans. Moreover, proliferative, antioxidative, anti-inflammatory, and calcification properties of these scaffolds on human dental pulp stem cells (hDPSCs) were evaluated. The results showed that PCL/Gel/CUR scaffold had antibacterial effects. Also, these CUR-based scaffolds had significant inhibitory effects on the expression of tumor necrosis factor α and DCF from inflamed hDPSCs (p < 0.05).Moreover, the induction of mineralization in hDPSCs significantly increased after seeding on CUR-based scaffolds (p < 0.05). Based on these findings, the investigated CUR-loaded material was fabricated successfully and provided an appropriate structure for the attachment and proliferation of hDPSCs. It was found that these scaffolds had antimicrobial, antioxidant, and anti-inflammatory characteristics and could induce mineralization in hDPSCs, which is essential for healing and repairing the injured dentin-pulp complex.

Synthesis, characterization, and evaluation of Hesperetin nanocrystals for regenerative dentistry

“…Hesperetin is known for its anti-inflammatory and antioxidative properties as well as other flavonoids 6 , 8 , 9 , 33 . Pinho-Ribeiro et al evaluated hesperidin in the rodent model to decrease inflammation-associated pain.…”

“…According to the results, hesperetin inhibited oxidative stress and inflammatory signals using down-regulation of critical cytokines in the inflammatory cascade, including TNF-, IL1ß, IL-6, and IL 10 35 , 36 . These interleukins and cytokines are widely involved in different inflammatory responses in the body, such as tooth and bone inflammation 6 , 9 . In the current study, human dental pulp stem cells were treated with both hesperetin and nano-hesperetin and inflamed by bacterial LPS and.…”

“…Hesperetin (3′,5,7-trihydroxy-4-methoxyflavanone) is a metabolite of hesperidin, a flavanone subgroup of flavonoids 7 , 8 . This natural ingredient indicates a wide range of biological activities such as antioxidative and anti-inflammatory effects as well as other flavonoids 6 , 9 – 13 . Moreover, hesperetin is an accessible and cost–benefit natural ingredient suggested as a bioactive substance for bone and Cartilage repair 7 , 14 .…”

Injectable CNPs/DMP1-loaded self-assembly hydrogel regulating inflammation of dental pulp stem cells for dentin regeneration

“…Therefore, developing new agents with the ability of anti-inflammatory, protect pulp stem cells and rapidly mobilize hDPSCs to promote the repairment of damaged pulp tissue, is still essential [ 20 ]. With the development of the material field, researchers have found that many biological materials can inhibit pulpitis and enhance the differentiation ability of hDPSCs, Such as baicalensis [ 21 ] and Curcumin [ 22 ] isolated from medicinal plant. In addition to this, many nanomaterials have also shown excellent anti-inflammatory ability, such as folic acid modified silver nanoparticles (FA-AgNPs) and [ 23 ]gold nanoparticles (AuNPs) [ 24 ].…”

Impact of curcumin loading on the physicochemical, mechanical and antimicrobial properties of a methacrylate-based experimental dental resin

“…It has also previously been shown to have an antimicrobial activity following aPDT against both cariogenic planktonic cells 25 , 30 , 31 and, to a lesser degree, cariogenic biofilms 28 , 30 , 32 , 33 when applied as a solution. Furthermore, curcumin has been shown to either have no inhibitory effect 34 or promote cell viability 35 of dental pulp cells. While these earlier investigations have shown the significant potential of curcumin as a PS in aPDT in direct contact with the cells, the prevention and management of dental caries would significantly benefit from a more site-specific and sustained antimicrobial effect, particularly on critical tooth-restoration interfaces susceptible to recurrent caries.…”