Isoflavonoids from Brazilian red propolis down‐regulate the expression of cancer‐related target proteins: A pharmacogenomic analysis

Nani, Bruno Dias; Franchin, Marcelo; Lazarini, Josy Goldoni; Freires, Irlan Almeida; Cunha, Marcos Guilherme da; Bueno‐Silva, Bruno; Alencar, Severino Matias de; Murata, Ramiro Mendonça; Rosalen, Pedro Luiz

doi:10.1002/ptr.6016

Cited by 23 publications

(14 citation statements)

References 17 publications

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“…Both neovestitol and vestitol were reported to modulate the NF-κB pathway in lipopolysaccharide (LPS)-activated macrophages [ 35 , 36 ] and decreased neutrophil migration, rolling and adhesion, by reducing the expression of ICAM-1 in an in vivo LPS-induced acute peritonitis model [ 37 , 38 ]. Lastly, a recent study also showed that these compounds downregulated the expression of proteins commonly used as targets for cancer treatment [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Brazilian Red Propolis Is as Effective as Amoxicillin in Controlling Red-Complex of Multispecies Subgingival Mature Biofilm In Vitro

et al. 2020

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This study investigated the effects of Brazilian Red Propolis (BRP) extract on seven-day-old multispecies subgingival biofilms. Mixed biofilm cultures containing 31 species associated with periodontal health or disease were grown for six days on a Calgary device. Then, mature biofilms were treated for 24 h with BRP extract at different concentrations (200–1600 µg/mL), amoxicillin (AMOXI) at 54 µg/mL (positive control) or vehicle (negative control). Biofilm metabolic activity was determined by colorimetry, and bacterial counts/proportions were determined by DNA–DNA hybridization. Data were analyzed by Kruskal–Wallis and Dunn’s tests. Treatment with BRP at 1600, 800 and 400 μg/mL reduced biofilm metabolic activity by 56%, 56% and 57%, respectively, as compared to 65% reduction obtained with AMOXI. Mean total cell counts were significantly reduced in all test groups (~50–55%). Lower proportions of red, green and yellow complex species were observed upon treatment with BRP (400 µg/mL) and AMOXI, but only AMOXI reduced the proportions of Actinomyces species. In conclusion, BRP extract was as effective as AMOXI in killing seven-day-old multispecies biofilm pathogens and did not affect the levels of the host-compatible Actinomyces species. These data suggest that BRP may be an alternative to AMOXI as an adjunct in periodontal therapy. In vivo studies are needed to validate these results.

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Section: Discussionmentioning

confidence: 99%

Brazilian Red Propolis Is as Effective as Amoxicillin in Controlling Red-Complex of Multispecies Subgingival Mature Biofilm In Vitro

et al. 2020

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“…The histone H3 is located in the nucleosome and is a unit of the chromatin. This structure maintains the DNA condensation in the cell during mitosis and is essential for the correct separation of sister chromatids and the formation of two daughter cells [ 31 ]. Figure 1 shows a schematic visualization of the depicted signaling pathways and their main elements.…”

Section: Mechanisms Of Anticancer Activity Of Propolis and Its Compoundsmentioning

confidence: 99%

Anticancer Activity of Propolis and Its Compounds

Forma

Bryś

2021

Nutrients

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Propolis is a natural material that honey bees (Apis mellifera) produce from various botanical sources. The therapeutic activity of propolis, including antibacterial, antifungal, and anti-inflammatory effects, have been known since antiquity. Cancer is one of the major burdens of disease worldwide, therefore, numerous studies are being conducted to develop new chemotherapeutic agents and treatments for cancer. Propolis is a rich source of biologically active compounds, which affect numerous signaling pathways regulating crucial cellular processes. The results of the latest research show that propolis can inhibit proliferation, angiogenesis, and metastasis of cancer cells and stimulate apoptosis. Moreover, it may influence the tumor microenvironment and multidrug resistance of cancers. This review briefly summarizes the molecular mechanisms of anticancer activity of propolis and its compounds and highlights the potential benefits of propolis to reduce the side effects of chemotherapy and radiotherapy.

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“…Formononetin is the main isoflavone present in red propolis samples [23,24]. Other compounds identified in the fractions and extracts from Brazilian red propolis, such as vestitol, neovestitol, biochanin A and liquiritigenina, are also considered important markers and have been associated with different biological effects [25,26].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the antioxidant profile and cytotoxic activity of red propolis extracts from different regions of northeastern Brazil obtained by conventional and ultrasound-assisted extraction

et al. 2019

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Propolis is a complex mixture of resinous and balsamic material collected from the exudates of plants, shoots, and leaves by bees. This study evaluated red propolis extracts obtained by conventional (ethanolic) extraction and ultrasound-assisted extraction of six samples from different regions of northeastern Brazil. The total phenolic compounds and flavonoids, in vitro antioxidant activity, concentration of formononetin and kaempferol and the cytotoxicity against four human tumor cell lines were determined for all twelve obtained extracts. Significant variations in the levels of the investigated compounds were identified in the red propolis extracts, confirming that the chemical composition varied according to the sampling region. The extraction method used also influenced the resulting propolis compounds. The highest concentration of the compounds of interest and the highest in vitro antioxidant activity were exhibited by the extracts obtained from samples from state of Alagoas. Formononetin and kaempferol were identified in all samples. The highest formononetin concentrations were identified in extracts obtained by ultrasound, thus indicating a greater selectivity for the extraction of this compound by this method. Regarding cytotoxic activity, for the HCT-116 line, all of the extracts showed an inhibition of greater than 90%, whereas for the HL-60 and PC3 lines, the minimum identified was 80%. In general, there was no significant difference (p>0.05) in the antiproliferative potential when comparing the extraction methods. The results showed that the composition of Brazilian red propolis varies significantly depending on the geographical origin and that the method used influences the resulting compounds that are present in propolis. However, regardless of the geographical origin and the extraction method used, all the red propolis samples studied presented great biological potential and high antioxidant activity. Furthermore, the ultrasound-assisted method can be efficiently applied to obtain extracts of red propolis more quickly and with high concentration of biomarkers of interest.

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Isoflavonoids from Brazilian red propolis down‐regulate the expression of cancer‐related target proteins: A pharmacogenomic analysis

Cited by 23 publications

References 17 publications

Brazilian Red Propolis Is as Effective as Amoxicillin in Controlling Red-Complex of Multispecies Subgingival Mature Biofilm In Vitro

Brazilian Red Propolis Is as Effective as Amoxicillin in Controlling Red-Complex of Multispecies Subgingival Mature Biofilm In Vitro

Anticancer Activity of Propolis and Its Compounds

Evaluation of the antioxidant profile and cytotoxic activity of red propolis extracts from different regions of northeastern Brazil obtained by conventional and ultrasound-assisted extraction

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