Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress

Sá, Rafael A. de; Castro, Frederico Augusto Vieira de; Eleuthério, Elis C. A.; Souza, R. M.; Silva, Joaquim F. M. da; Pereira, Marcos D.

doi:10.1590/s1517-83822013005000062

Cited by 36 publications

(14 citation statements)

References 43 publications

(77 reference statements)

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“…braziliensis in vitro [64]. All of the extracts herein confirmed the antioxidant effects of propolis, as evidenced by significant decreases in nitric oxide and superoxide production [65–69]. In addition, our results showed increased superoxide dismutase (SOD) activity following treatment by alcoholic and glycolic extracts.…”

Section: Discussionsupporting

confidence: 75%

Parasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extracts

Rebouças-Silva

Celes

Lima

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Treatments based on antimonials to cutaneous leishmaniasis (CL) entail a range of toxic side effects. Propolis, a natural compound widely used in traditional medical applications, exhibits a range of biological effects, including activity against infectious agents. The aim of this study was to test the potential leishmanicidal effects of different propolis extracts against Leishmania (Viannia) braziliensis promastigotes and intracellular amastigotes in vitro. Stationary-phase L. (V) braziliensis promastigotes were incubated with medium alone or treated with dry, alcoholic, or glycolic propolis extract (10, 50, or 100 μg/mL) for 96 h. Our data showed that all extracts exhibited a dose-dependent effect on the viability of L. (V) braziliensis promastigotes, while controlling the parasite burden inside infected macrophages. Dry propolis extract significantly modified the inflammatory profile of murine macrophages by downmodulating TGF-β and IL-10 production, while upmodulating TNF-α. All three types of propolis extract were found to reduce nitric oxide and superoxide levels in activated L. braziliensis-infected macrophages. Altogether, our results showed that propolis extracts exhibited a leishmanicidal effect against both stages of L. (V) braziliensis. The low cell toxicity and efficient microbicidal effect of alcoholic or glycolic propolis extracts make them candidates to an additive treatment for cutaneous leishmaniasis.

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Section: Discussionsupporting

confidence: 75%

Parasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extracts

Rebouças-Silva

Celes

Lima

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…fumigatus wild type, Δ rtfA , complementation, and OE rtfA strains to various concentrations of menadione. Menadione produces ROS once it enters the cell, creating an environment that is under oxidative stress [73]. Our results conclusively showed that rtfA is important for the response of A .…”

Section: Discussionsupporting

confidence: 68%

rtfA controls development, secondary metabolism, and virulence in Aspergillus fumigatus

et al. 2017

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Invasive aspergillosis by Aspergillus fumigatus is a leading cause of infection-related mortality in immune-compromised patients. In order to discover potential genetic targets to control A. fumigatus infections we characterized rtfA, a gene encoding a putative RNA polymerase II transcription elongation factor-like protein. Our recent work has shown that the rtfA ortholog in the model fungus Aspergillus nidulans regulates morphogenesis and secondary metabolism. The present study on the opportunistic pathogen A. fumigatus rtfA gene revealed that this gene influences fungal growth and conidiation, as well as production of the secondary metabolites tryptoquivaline F, pseurotin A, fumiquinazoline C, festuclavine, and fumigaclavines A, B and C. Additionally, rtfA influences protease activity levels, the sensitivity to oxidative stress and adhesion capacity, all factors important in pathogenicity. Furthermore, rtfA was shown to be indispensable for normal virulence using Galleria mellonella as well as murine infection model systems.

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“…Many studies have shown, that phenolic compounds demonstrate the capacity to inhibition of lipid peroxidation in cells (Meng et al, 2017). This effect was also observed in yeast cells exposed to cadmium and blueberry extracts (Opera et al, 2014), yeast, and its mutant strains devoid of antioxidant enzymes and treated with cadmium and resveratrol (Dani et al, 2008), as well as yeast treated with propolis and quercetin in exposure to H 2 O 2 (de Sa et al, 2013).…”

Section: The Effect Of Onion Skin Extract On the Total Content Of Gmentioning

confidence: 87%

“…Cells were counted using Thoma counting chamber. The experiment was performed in triplicate (Dani et al, 2008;Tongul & Tarhan, 2016;Żyracka, 2014).…”

Section: Cell Growth Measurementmentioning

confidence: 99%

Onion skin extract as a protective agent against oxidative stress in Saccharomyces cerevisiae induced by cadmium

Piechowiak

Balawejder

2019

J Food Biochem

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Onion skin extract is a source of bioactive compounds demonstrating a high antioxidant activity. The purpose of this research was to investigate the effect of onion skin extract at the level of oxidative stress markers in Saccharomyces cerevisiae exposed to cadmium. The generation of reactive oxygen species (ROS), antioxidant enzymes activity, total content of glutathione, and the level of lipid peroxidation in yeast cells were determined. Research showed that onion skin extract protected the yeast cells from the occurrence of oxidative stress induced by cadmium. Cells treated with onion skin extract and cadmium were characterized by lower generation of ROS, antioxidant enzymes activity, lipid peroxidation, and higher level of glutathione content than cells treated with cadmium only. However, the protective effect was dependent on the onion skin extract concentration in yeast medium. Practical applications The presented studies are of comprehensive character regarding antioxidant properties of the onion skin extract, using Saccharomyces cerevisiae as a model organism. The research results give the opportunity to conduct further research on the use of onion skin extract in functional food or dietary supplements production, as a cellular protector against negative consequence of oxidative stress. It should also be mentioned that the major research material was onion skin which is a troublesome waste material for onion producers. Consequently, the study result will, to a certain extent provide an opportunity to increase interest in the problem of rational use of the waste material, according to a sustainable development idea.

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Brazilian propolis protects Saccharomyces cerevisiae cells against oxidative stress

Cited by 36 publications

References 43 publications

Parasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extracts

Parasite Killing of Leishmania (V) braziliensis by Standardized Propolis Extracts

rtfA controls development, secondary metabolism, and virulence in Aspergillus fumigatus

Onion skin extract as a protective agent against oxidative stress in Saccharomyces cerevisiae induced by cadmium

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