Onion skin extract as a protective agent against oxidative stress in Saccharomyces cerevisiae induced by cadmium

Abstract

Onion skin extract is a source of bioactive compounds demonstrating a high antioxidant activity. The purpose of this research was to investigate the effect of onion skin extract at the level of oxidative stress markers in Saccharomyces cerevisiae exposed to cadmium. The generation of reactive oxygen species (ROS), antioxidant enzymes activity, total content of glutathione, and the level of lipid peroxidation in yeast cells were determined. Research showed that onion skin extract protected the yeast cells from the occurrence of oxidative stress induced by cadmium. Cells treated with onion skin extract and cadmium were characterized by lower generation of ROS, antioxidant enzymes activity, lipid peroxidation, and higher level of glutathione content than cells treated with cadmium only. However, the protective effect was dependent on the onion skin extract concentration in yeast medium. Practical applications The presented studies are of comprehensive character regarding antioxidant properties of the onion skin extract, using Saccharomyces cerevisiae as a model organism. The research results give the opportunity to conduct further research on the use of onion skin extract in functional food or dietary supplements production, as a cellular protector against negative consequence of oxidative stress. It should also be mentioned that the major research material was onion skin which is a troublesome waste material for onion producers. Consequently, the study result will, to a certain extent provide an opportunity to increase interest in the problem of rational use of the waste material, according to a sustainable development idea.

Characterization of Novel Synthetic Polyphenols: Validation of Antioxidant and Vasculoprotective Activities

“…None of the tested compounds nor control antioxidants, vitamin C or resveratrol, recover completely growth rate, as mean values were significantly different from unstressed yeast (YPD(CTRL)). This recovery of growth rate is similar to that obtained with other synthetic [ 16 ] or food-derived phenolic compounds like onion skin extracts or cocoa polyphenols [ 30 , 37 ] or elements like selenium [ 38 ]. When comparing the carrying capacity (k) ( Figure 4 B), all three compounds at each single dose tested were able to recover the carrying capacity of yeast cells similarly as control antioxidants, and even to the same levels of unstressed yeast cells.…”

Extraction of Antioxidant Compounds from Blueberry Fruit Waste and Evaluation of Their In Vitro Biological Activity in Human Keratinocytes (HaCaT)

“…Mean values (n = 6) with standard error (error bars) with *, **, and *** are statistically different from the respective control at p < 0.05, p < 0.01, and p < 0.001, respectively (one-way ANOVA, Tukey test). On the figure: E, blueberry waste extract; C, control sample ◂ appropriate enzymes involved in the neutralization of ROS (Matés et al 1999;Piechowiak and Balawejder 2019;Yan et al 2020). A similar correlation was found in the study under discussion.…”

The Impact of Organic Fertilizer Produced with Vegetable Residues in Lettuce (Lactuca sativa L.) Cultivation and Antioxidant Activity

“…Onion skin is one of the primary residues generated during processing and this residue is used as a supplement in bread production, quercetin purification, and pigment extraction [3].…”