Brassica biotechnology: Progress in cellular and molecular biology

Cardoza, Vinitha; Stewart, C. Neal

doi:10.1079/ivp2004568

Cited by 109 publications

(60 citation statements)

References 134 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…High-frequency regeneration in cotyledon (90%) was achieved in combination of TDZ and NAA. Genotype specificity is a limiting factor in Brassica tissue culture and regeneration, which severely limits the germplasm that can be manipulated or improved (Cardoza and Stewart 2004). In addition, there is lack of information on organogenesis of cabbage particularly "KY Cross" using phenyl-urea type cytokinin such as TDZ.…”

mentioning

confidence: 99%

Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) ^|^#8220;KY Cross^|^#8221;

Ravanfar

Salim

Aziz

et al. 2014

Plant Biotechnology

View full text Add to dashboard Cite

In this study, the effects of phenyl-urea (Thidiazuron) and adenine (6-benzylaminopurine) and 6-y,y,dimethylally-amino purine type cytokinins alone or in combination with indole-3-butyric acid on shoot regeneration from hypocotyl and cotyledonary explants of Brassica oleracea ssp. capitata "KY Cross" were investigated. For hypocotyl explants, medium containing 2.27 µM Thidiazuron showed the highest mean number (18.15) of separable shoots per explant with 80% shoot formation. In the case of cotyledonary explants, the highest mean number of shoots (3.03) was obtained on medium containing 12.30 µM 6-y,y,dimethylally-amino purine with a percentage of 56.67% shoot formation. Plantlets were successfully acclimatized with 70% survival in potting medium consisting of coconut husk+vermicompost (7 : 1 v/v). The regeneration system developed herewith will be a valuable tool for genetic improvement of cabbage "KY Cross".

show abstract

mentioning

confidence: 99%

Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) ^|^#8220;KY Cross^|^#8221;

Ravanfar

Salim

Aziz

et al. 2014

Plant Biotechnology

View full text Add to dashboard Cite

show abstract

“…In B. napus (Cardoza and Stewart 2003), increasing the percentage of gelling agent (from 0.8% phytagar to 1.2%) in the shoot elongation medium reduced the relative humidity of the culture vessel and reduced the hyperhydricity. Ethylene is another key factor in optimizing the tissue culture conditions for some Brassica species (Cardoza and Stewart 2004). Silver nitrate has been used to reduce the hyperhydricity in a range of Brassica species, such as B. rapa (Yang et al 2004) and B. napus (Tang et al 2003).…”

Section: Hyperhydricity and Tissue Necrosismentioning

confidence: 99%

Advances in in vitro culture of the Brassicaceae crop plants

Park¹,

Ahmed

Kim

et al. 2012

Journal of Plant Biotechnology

View full text Add to dashboard Cite

“…This indicated that salt tolerance of the crop genotypes is an important factor in the arid and semi-arid conditions. Canola, which is most often B. napus, has received much attention worldwide and may soon be the most popular oilseed crop (Cardoza & Stewart, 2004). Brassica napus L. is an amphidiploid (2n=38) and is believed to have arisen by interspecific hybridization between the diploid species B. rapa (2n=20) and B. oleracea (2n=18) and it is predominantly self-pollinated with about 30% out crossing (Diers & Osborn, 1994).…”

Section: Introductionmentioning

confidence: 99%

Devolopment of Some Canola Genotypes to Salinity Tolerance Using Tissue Culture Technique

Abdrabou¹,

Fergani²,

Azzam³

et al. 2017

Egypt. J. Agron.

View full text Add to dashboard Cite

, to study the variation among canola varieties in response to salt tolerance through tissue culture propagation. This work was aimed to establish a regeneration protocol for canola genotypes, production of salt-tolerance plants through selection of salttolerance callus via somaclonal variation and subsequent regeneration of plantlets from such callus. Four genotypes of canola were used for establishment a regeneration protocol, Bingo and Torpe were uesed as highly salt tolerant genotypes while, Conny and Siberian as susceptible genotypes. The results indicated that MS medium supplemented with 2 mg/l 2,4-D was the successive medium for callus formation and shoot regeneration was obtained from MS medium supplemented with 5 mg/l BA + 0.05 NAA, while, 1mg/l IBA was the better growth regulator for rooting.For in vitro selection of salt tolerant calli, Torpe (genotype overcomes the others in calli survival percentages under 8000, 12000 and 16000 ppm NaCl concentrations. The results of shoot frequency from tolerant calli cleared that Torpe genotype recorded the highest value of shoot percentage followed by Bingo genotype, whereas, Conny followed by Siberian genotypes were recorded the lowest values. At 16000 ppm NaCl concentration, only tolerant calli of Torpe genotype was able to initiat shoot under this concentration. For acclamatization after regeneration from the tolerant calli, only five salt tolerant plantlets developed from Torpe genotype and eight salt tolerant plantlets developed from Siberian genotype that succeeded to complete their life cycle and reached maturity stage and produced seeds. These regenerated genotypes were referred as T1, T2, T3, T4 and T5, the five regenerated plants developed from Torpe genotype that tolerate NaCl concentration up to 16000 ppm, as well as, S1, S2, S3, S4, S5, S6, S7 and S8, the eight regenerated plants developed from Siberian genotype that tolerate NaCl concentration up to 12000 ppm.

show abstract

Brassica biotechnology: Progress in cellular and molecular biology

Cited by 109 publications

References 134 publications

Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) ^|^#8220;KY Cross^|^#8221;

Influence of phenyl-urea and adenine-type cytokinins on direct adventitious shoot regeneration of cabbage (Brassica oleracea subsp. capitata) ^|^#8220;KY Cross^|^#8221;

Advances in in vitro culture of the Brassicaceae crop plants

Devolopment of Some Canola Genotypes to Salinity Tolerance Using Tissue Culture Technique

Contact Info

Product

Resources

About