Advances in in vitro culture of the Brassicaceae crop plants

Park, Jong In; Ahmed, Nasar Uddin; Kim, Hye Ran; Nou, Ill Sup

doi:10.5010/jpb.2012.39.1.013

Cited by 6 publications

(4 citation statements)

References 74 publications

(69 reference statements)

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“…Raphanus sativus , another vegetable of the same family, is considered recalcitrant and only few reports are available. Advances in regeneration and transformation in this group of crops have been reviewed elsewhere ( Vinterhalter et al, 2007 ; Klocke et al, 2010 ; Sparrow et al, 2011 ; Park et al, 2012 ; Kumar and Srivastava, 2016 ). With the aim to avoid tissue culture systems, similarly to the related species Arabidopsis thaliana, in planta transformation systems based on floral dipping or flower bud microinjection have been also attempted in various genotypes ( Curtis and Nam, 2001 ; Sparrow et al, 2011 ).…”

Section: Regeneration and Transformationmentioning

confidence: 99%

Genetic Transformation and Genomic Resources for Next-Generation Precise Genome Engineering in Vegetable Crops

2017

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In the frame of modern agriculture facing the predicted increase of population and general environmental changes, the securement of high quality food remains a major challenge to deal with. Vegetable crops include a large number of species, characterized by multiple geographical origins, large genetic variability and diverse reproductive features. Due to their nutritional value, they have an important place in human diet. In recent years, many crop genomes have been sequenced permitting the identification of genes and superior alleles associated with desirable traits. Furthermore, innovative biotechnological approaches allow to take a step forward towards the development of new improved cultivars harboring precise genome modifications. Sequence-based knowledge coupled with advanced biotechnologies is supporting the widespread application of new plant breeding techniques to enhance the success in modification and transfer of useful alleles into target varieties. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 system, zinc-finger nucleases, and transcription activator-like effector nucleases represent the main methods available for plant genome engineering through targeted modifications. Such technologies, however, require efficient transformation protocols as well as extensive genomic resources and accurate knowledge before they can be efficiently exploited in practical breeding programs. In this review, we revise the state of the art in relation to availability of such scientific and technological resources in various groups of vegetables, describe genome editing results obtained so far and discuss the implications for future applications.

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Section: Regeneration and Transformationmentioning

confidence: 99%

Genetic Transformation and Genomic Resources for Next-Generation Precise Genome Engineering in Vegetable Crops

2017

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“…Oilseed rape (Brassica napus L.) is one of the most important oilseed crops in the world. Even though oilseed rape has been a significant target for breeding efforts aimed at altering the composition of fatty acids for many years, the adoption of modern biotechnological approaches still faces challenges, primarily due to genotype-dependent variations in in vitro regeneration efficiency (Park et al, 2012;Farooq et al, 2019). One of the obstacles hindering the successful application of transformation approaches is the response of oilseed rape tissue to in vitro regeneration.…”

Section: Introductionmentioning

confidence: 99%

Biochemical Responses in Agrobacterium-Infected Oilseed Rape Explants During Early Stages of Regeneration in the Presence of Dithiothreitol

Dronzeková,

Karas,

Boszoradová

et al. 2024

J microb biotech food sci

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We investigated the impact of exogenous dithiothreitol on the activities of enzymatic antioxidants ascorbate peroxidase and catalase, and the levels of non-enzymatic antioxidants and osmoprotectant proline, and total soluble sugars in Agrobacterium-infected and non-infected (control) explants of oilseed rape (Brassica napus L.) after the 3rd and 17th day of in vitro culture. This investigation was conducted with or without dithiothreitol at a concentration of 1 mg/L. Dithiothreitol is a sulphur-containing compound that act as a reducing agent, impacting the redox environment within plant cells. Explants were obtained from six-day-old hypocotyls of the cultivars Ability and Lancia, and infected with Agrobacterium tumefaciens GV3101 carrying the binary vector. The application of dithiothreitol into co-cultivation and regeneration media led to a significant decrease in the activities of ascorbate peroxidase and catalase in both infected and non-infected explants of both cultivars. In contrast, explants regenerated without dithiothreitol exhibited a significant increase in ascorbate peroxidase activity and a decrease in catalase activity, although the decrease in catalase activity was not as pronounced. The proline content increased significantly in all analysed explants, with the highest increase observed in explants regenerated in the presence of dithiothreitol. This suggests that dithiothreitol might indirectly contribute to the reduced formation of reactive oxygen species, which is generally negatively correlated with transformation efficiency.

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“…Conventional breeding programmes have been mainly attempted to produce oilseed rape varieties with modified content of fatty acids. The main obstacles to the application of modern methods such as gene transfer are a highly variable in vitro regeneration efficiency and genotype specificity of oilseed rape (Park et al, 2012). Until now, only the genetically modified Argentine Canola has been authorized (https://www.isaaa.org, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Some oilseed rape cultivars remain recalcitrant to in vitro. Ethylene accumulation, high humidity in culture vessels, nutrient-rich media or high doses of cytokinins and auxins appear to be a serious problem for regeneration of oilseed rape (Park et al, 2012;Paladi et al, 2017;Farooq et al, 2019). Ethylene is a plant hormone that regulates many developmental processes and modulate stress responses (Polko and Kieber, 2019).…”

Section: Introductionmentioning

confidence: 99%

Effect of Silver Nitrate on in Vitro Regeneration and Antioxidant Responses of Oilseed Rape Cultivars (Brassica Napus L.)

Ramadan

Karas

Ranušová

et al. 2021

JMBFS

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Plant regenerationWe studied a stimulatory effect of silver nitrate on callus formation and shoot regeneration of five oilseed rape commercial varieties Ability, Lagonda, Lancia, Menthal and Mirakel (Brassica napus L.). Hypocotyls were induced to organogenesis on the media supplemented with silver nitrate at concentration 5 mg.L 1 and without silver nitrate as a control. After 2 weeks of cultivation, calli on the media with silver nitrate became deep green in colour. First shots appeared after 4 weeks of cultivation on the media with silver nitrate. Calli formed on the control media were pale green and did not form any shoots or at very low efficiency. Silver nitrate improved regeneration efficiency from 0-0.8% to 8.3-10% in varieties Albina, Lancia and Menthal. Explants of Lagonda and Mirakel did not form any calli and these varieties appeared to be recalcitrant. Analyses of six weeks-old calli showed that silver nitrate increased concentration of photosynthetic pigments, which is a good prerequisite for cell regeneration. Silver nitrate increased malondialdehyde level and induced changes in enzyme activities of antioxidants ascorbate peroxidase and catalase; and in the accumulation of non-enzymatic antioxidant proline; in the genotype dependant manner. The results suggest that an activation of stress response was necessary to obtain shoots with higher frequency in the varieties Albina, Lancia and Menthal. ARTICLE INFO

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Advances in in vitro culture of the Brassicaceae crop plants

Cited by 6 publications

References 74 publications

Genetic Transformation and Genomic Resources for Next-Generation Precise Genome Engineering in Vegetable Crops

Genetic Transformation and Genomic Resources for Next-Generation Precise Genome Engineering in Vegetable Crops

Biochemical Responses in Agrobacterium-Infected Oilseed Rape Explants During Early Stages of Regeneration in the Presence of Dithiothreitol

Effect of Silver Nitrate on in Vitro Regeneration and Antioxidant Responses of Oilseed Rape Cultivars (Brassica Napus L.)

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