, to study the variation among canola varieties in response to salt tolerance through tissue culture propagation. This work was aimed to establish a regeneration protocol for canola genotypes, production of salt-tolerance plants through selection of salttolerance callus via somaclonal variation and subsequent regeneration of plantlets from such callus. Four genotypes of canola were used for establishment a regeneration protocol, Bingo and Torpe were uesed as highly salt tolerant genotypes while, Conny and Siberian as susceptible genotypes. The results indicated that MS medium supplemented with 2 mg/l 2,4-D was the successive medium for callus formation and shoot regeneration was obtained from MS medium supplemented with 5 mg/l BA + 0.05 NAA, while, 1mg/l IBA was the better growth regulator for rooting.For in vitro selection of salt tolerant calli, Torpe (genotype overcomes the others in calli survival percentages under 8000, 12000 and 16000 ppm NaCl concentrations. The results of shoot frequency from tolerant calli cleared that Torpe genotype recorded the highest value of shoot percentage followed by Bingo genotype, whereas, Conny followed by Siberian genotypes were recorded the lowest values. At 16000 ppm NaCl concentration, only tolerant calli of Torpe genotype was able to initiat shoot under this concentration. For acclamatization after regeneration from the tolerant calli, only five salt tolerant plantlets developed from Torpe genotype and eight salt tolerant plantlets developed from Siberian genotype that succeeded to complete their life cycle and reached maturity stage and produced seeds. These regenerated genotypes were referred as T1, T2, T3, T4 and T5, the five regenerated plants developed from Torpe genotype that tolerate NaCl concentration up to 16000 ppm, as well as, S1, S2, S3, S4, S5, S6, S7 and S8, the eight regenerated plants developed from Siberian genotype that tolerate NaCl concentration up to 12000 ppm.

White coats worn by professionals in the medical field (students of health) or the work in the laboratory (students of Science), goal protect clothing from microbes. In this study was determined the type of microbial contamination on the white coats and the potential risks of microbes. The study was done by a questionnaire for students and samples swabs of coats for 80 students from the Fac. of Health and Science. The results explained the presence of pathogenic bacteria causing inflammatory and dimples contaminated white coats, such as Erysipelothrio rhusiopathiae causing the h time skin, Micrococcus luteues causing chronic inflammatory skin ; bacterial meningitis and blood contamination unidentified Organism and Kocuria kristinae causing infections of the urinary tract infections, gallbladder and opportunistic bacteria and the spread of nosocomial infections, one of opportunistic pathogens. As well as recorded high proportion of serious fungi that cause various diseases and contamination of food such as Aspergillus niger ; Helminthosporium spp, Fusarium SPP, and Alternaria alternate . This study suggests that a large proportion of white coats may be a carrier of serious morbidity among female students in colleges is different, especially when not . There are laws and regulations in organizing wear these coats and the way to carry and learn how the daily cleaning of .

Two field experiments were carried out in Agric. Expt. Farm at Shalakan, Kaleobia Governorate, Cairo, Egypt during 2010/2011 and 2011/2012 growing seasons to study the response of some bread wheat cultivars to sowing dates. Each experiment included 15 treatments which were the combination between three wheat cultivars (Giza 168, Sakha 93 and Gemmiza 9) and five sowing dates (1 st October, 16 th October, 1 st November, 16 th November and 1 st December). The experimental design used was split plot design in 6 replications. The data revealed that sowing dates and cultivars both significantly affected wheat yield and its components. Sowing on 1 st November exhibited significant maximum plant height, number of spike/m 2 , main spike length and weight, grains number of main spike as well as grain, straw and biological yields as compared to early or late sowing in the season. Concerning wheat cultivars, Gemmiza 9 gave significantly highest yield and its components in comparison to Sakha 93 and Giza 168. The effect of the interaction between wheat cultivars and sowing dates were significantly differed in plant height, main spike length and weight, grain weight/spike as well as grain, straw and biological yields as well as GCPY. Gemmiza 9 exhibited the significant highest parameters when sown on 1 st November as compared to the other studied two cultivars. The data revealed that sowing Gemmiza 9 wheat cultivar on 1 st November was the most suitable environmental conditions for growing wheat in Kaluobia Governorate.

Egyptian Journal of Botany http://ejbo.journals.ekb.eg/ 2041 C OTTON is known as the king of fibers and an important source of oil. The aim of this study was to evaluate the mitigating effects of three osmoprotectants, including glycine betaine (GB), proline, and chitosan each at two concentrations of 400 and 200 parts per million (ppm), 100 and 50 ppm, and 300 and 100 ppm, respectively, on water deficit stress after irrigation intervals of 10-, 15-, and 20-days, relative to the untreated (control) plants. Data indicated that prolonged irrigation interval significantly decreased growth, yield, yield components, fiber parameters, total chlorophyll, Chl. a, and Chl. b, while, proline and total soluble sugars significant increased. All drought tolerance inducers showed significant increases in cotton growth and productivity traits. Generally, GB treated plants at 400 ppm showed superior traits of all studied parameters. For example, the interaction between irrigation intervals and GB application caused significant effects on growth and productivity as well as fiber quality and chemical properties. The application of drought tolerance inducers mitigated the effects of prolonged irrigation intervals in cotton, and all drought tolerance inducers achieved higher yield and yield component values at 15 days irrigation interval compared with the control at 10 days irrigation interval. Application of 400 ppm GB could improve cotton plants endurance against the negative effect of prolonged water intervals.