Brain-derived neurotrophic factor (BDNF) affects somatotrophic axis activity in sheep

The Effect of Leptin on the Blood Hormonal Profile (Cortisol, Insulin, Thyroid Hormones) of the Ewe in Acute Inflammation in Two Different Photoperiodical Conditions

“…The experimental procedure began 24 h following PMSG injection, so the ewes were in the follicular phase of the oestrous cycle. The same synchronization scheme was presented by Przybył, et al [ 61 ]. During the LD period, animal synchronization was not required, as the animals were in seasonal anoestrous.…”

Time-dependent effect of inflammation on the gene expression of pro-inflammatory cytokines and their receptors at the different levels of the somatotropic axis in ewe

“…Before the beginning of the experiment (12 h), animals were deprived of food. Before the experiment, the stage of the oestrous cycle of ewes was synchronized using an intra-vaginal sponge impregnated with 20 mg of a synthetic progesterone-like hormone, the Chronogest ® CR (Merck Animal Health, Boxmeer, The Netherlands) following the methodology outlined in Przybył et al (2021). After sponge removal, the ewes received an intramuscular injection of 500 IU pregnant mare's serum gonadotropin (PMSG) (Merck Animal Health, Boxmeer, The Netherlands).…”

Circadian and seasonal changes in the expression of clock genes in the ovine pars tuberalis

“…The trials were scheduled to take place approximately one month before the summer solstice LD photoperiod and winter solstice SD photoperiod. In the SD photoperiod, the oestrus cycle of 2-3year old ewes was synchronised using a Chronogest ® controlledrelease vaginal sponge for sheep (Merck Animal Health, Boxmeer, The Netherlands), following the methodology outlined in Przybył et al (2021). The experiments were specifically conducted on the 10 th day of the luteal phase, during oestradiol and progesterone plateau.…”