Bovine serum albumin release from novel chitosan-fluoro-aluminosilicate glass ionomer cement: Stability and cytotoxicity studies

Limapornvanich, Araya; Jitpukdeebodintra, Suwanna; Hengtrakool, Chanothai; Kedjarune‐Leggat, Ureporn

doi:10.1016/j.jdent.2009.05.007

Cited by 19 publications

(14 citation statements)

References 30 publications

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“…From our previous study, we found that chitosan added to GIC was able to prolong the release of BSA for 2 weeks, and also BSA by itself was able to reduce the toxicity of the material (13). In this study, we reduced the percentages of chitosan from 20% by weight from that previous study to 15% to reduce the viscosity of the cement.…”

Section: Discussionmentioning

confidence: 84%

“…BIO-GIC specimens also had significantly higher percentages of viable cells than GIC group at the second period. This result might come from the effect of chitosan and albumin, because in their study Limapornvanich et al (13) found that chitosan did not increase the cytotoxicity of GIC, and albumin might even reduce the cytotoxicity of GIC. The real mechanism of chitosan to reduce cytoxicity is not known, whereas BSA itself has the property to bind toxic chemical (25).…”

Section: Discussionmentioning

confidence: 95%

“…There have been some attempts to enhance sustained release of substances from this cement, such as casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) (11), chlorhexidine (12), and also, in particular, some proteins (13). Chitosan is a biocompatible, biodegradable natural biopolymer that is a copolymer of glucosamine and N-acetylglucosamine derived from chitin.…”

mentioning

confidence: 99%

“…In addition, calcium hydroxide-containing chitosan can sustain release of calcium ion for more than 30 days (17). Recently, our group discovered a novel chitosan-fluoroaluminosilicate GIC that can prolong the release of BSA without alteration of its molecular weight, and this cement did not increase toxicity to pulp cells (13). This material can prolong the release of protein, possibly as a result of the formation of a polyelectric complex between the cationic group of chitosan and the anionic group of poly(acrylic acid) (18).…”

mentioning

confidence: 99%

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Effect of Novel Chitosan-fluoroaluminosilicate Glass Ionomer Cement with Added Transforming Growth Factor Beta-1 on Pulp Cells

Rakkiettiwong¹,

Hengtrakool²,

Thammasitboon³

et al. 2011

Journal of Endodontics

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 95%

mentioning

confidence: 99%

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confidence: 99%

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Effect of Novel Chitosan-fluoroaluminosilicate Glass Ionomer Cement with Added Transforming Growth Factor Beta-1 on Pulp Cells

Rakkiettiwong¹,

Hengtrakool²,

Thammasitboon³

et al. 2011

Journal of Endodontics

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“…Researchers have demonstrated its great potential for a wide range of uses due to its versatile chemical and physical properties like biodegradability, biocompatibility, antimicrobial activity, nontoxicity. 67 …”

Section: Introductionmentioning

confidence: 99%

A Comparative Evaluation of Microleakage of Glass Ionomer Cement and Chitosan-modified Glass Ionomer Cement: An in vitro Study

Abraham

Thomas

Chopra

et al. 2014

International Journal of Clinical Pediatric Dentistry

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Objective: To do a comparative study of microleakage of glass ionomer cement (GIC) and chitosan modified glass ionomer cement and evaluate which exhibited lesser microleakage.Materials and methods: Sixty freshly extracted sound primary molar teeth were obtained. Two groups of samples were created for the study which comprised of group I (glass ionomer cement—GIC) and group II (Chitosan modified glass ionomer cement). Class V cavities were prepared on the buccal surfaces. All the tooth surfaces except the restoration and a 1 mm zone adjacent to its margins were covered with two coats of varnish. The specimens were then immersed in 2% basic fuschin dye solution for 24 hours. The teeth were sectioned into two halves buccolingually in an occlusoapical direction. Sections were viewed under stereomicroscope and the degree of microleakage was evaluated using specific scoring criteria. For comparative evaluation of microleakage scores between glass ionomer cement and chitosan modified cement, a nonparametric Mann-Whitney statistical analysis was done.Results: Statistical analysis showed no significant differences between groups I and II with the p-value at >0.05.Conclusion: Chitosan modified GIC holds great promise for general dentistry as a future restorative material with microleakage properties similar to or better than GIC.How to cite this article: Abraham D, Thomas AM, Chopra S, Koshy S. A Comparative Evaluation of Microleakage of Glass Ionomer Cement and Chitosan-modified Glass Ionomer Cement: An in vitro Study. Int J Clin Pediatr Dent 2014;7(1):6-10.

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Effect of novel chitosan-fluoroaluminosilicate resin modified glass ionomer cement supplemented with translationally controlled tumor protein on pulp cells

Wanachottrakul

Chotigeat

Kedjarune‐Leggat

2014

J Mater Sci: Mater Med

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Dental materials that can promote cell proliferation and function is required for regenerative pulp therapy. Resin modified glass ionomer cement (RMGIC), a broadly used liner or restorative material, can cause apoptosis to pulp cells mainly due to HEMA (2-hydroxyethyl methacrylate), the released residual monomer. Recent studies found that chitosan and albumin could promote release of protein in GIC while translationally controlled tumor protein (TCTP) has an anti-apoptotic activity against HEMA. The aim of this study was to examine the effect of chitosan and albumin modified RMGIC (Exp-RMGIC) supplemented with TCTP on pulp cell viability and mineralization. Exp-RMGIC+TCTP was composed of RMGIC powder incorporated with 15 % of chitosan, 5 % albumin and supplemented with TCTP mixed with the same liquid components of RMGIC. The effect of each specimen on pulp cells was examined using the Transwell plate. From the MTT assay, Exp-RMGIC+TCTP had the highest percentages of viable cells (P < 0.05) at both 24 and 74 h. Flow cytometry revealed that, after 24 h, Exp-RMGIC+TCTP gave the lowest percentages of apoptotic cells compared to other groups. There was no difference in alkaline phosphatase (ALP) activity among different formula of the specimens, while cells cultured in media with TCTP had higher ALP activity. Von Kossa staining revealed that RMGIC+TCTP, and Exp-RMGIC+TCTP had higher percentages of calcium deposit area compared to those without TCTP. It was concluded that Exp-RMGIC supplemented with TCTP had less cytotoxicity than RMGIC and can protect cells from apoptosis better than RMGIC supplemented with TCTP.

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Bovine serum albumin release from novel chitosan-fluoro-aluminosilicate glass ionomer cement: Stability and cytotoxicity studies

Cited by 19 publications

References 30 publications

Effect of Novel Chitosan-fluoroaluminosilicate Glass Ionomer Cement with Added Transforming Growth Factor Beta-1 on Pulp Cells

Effect of Novel Chitosan-fluoroaluminosilicate Glass Ionomer Cement with Added Transforming Growth Factor Beta-1 on Pulp Cells

A Comparative Evaluation of Microleakage of Glass Ionomer Cement and Chitosan-modified Glass Ionomer Cement: An in vitro Study

Effect of novel chitosan-fluoroaluminosilicate resin modified glass ionomer cement supplemented with translationally controlled tumor protein on pulp cells

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