Bovine herpesvirus type 1 (BHV-1) up-regulates telomerase activity in MDBK cells

Pagnini, Ugo; Martino, Luisa De; Montagnaro, Serena; Diodato, A.; Longo, Mariangela; Pacelli, F.; Pisanelli, Giuseppe; Iovane, G.

doi:10.1016/j.vetmic.2005.11.006

Cited by 9 publications

(5 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antibodies targeting p53 and p16 have been used as prognostic factors in human cutaneous SCCs [ 15 ] although no relationship has been found in cattle with OSCC [ 16 ]. Viruses including bovine papillomavirus and herpesvirus have been shown to induce preneoplastic lesions as well as neoplastic transformation [ 17 , 18 ]. Papillomaviruses are well known to cause proliferative lesions of the epidermis in the skin and mucosal membranes that may undergo malignant transformation [ 19 - 22 ].…”

Section: Introductionmentioning

confidence: 99%

Ocular squamous cell carcinoma in Holstein cows from the South of Brazil

Fornazari¹,

Kravetz²,

Kiupel³

et al. 2017

Vet World

View full text Add to dashboard Cite

Aim:The aim of this study was to investigate 10 cases of bovine ocular squamous cell carcinoma (OSCC) diagnosed in Holstein or Holstein-crosses cows.Materials and Methods:The investigation was performed exclusively in OSCC cases diagnosed in the State of Paraná and Santa Catarina. A combination of two previously existing histopathological classifications systems was used. The tissue samples were tested for immunoexpression of p53 and p16 and polymerase chain reaction (PCR) for bovine herpesvirus and papillomavirus.Results:A positive correlation between number of mitotic figures and tissue invasion was found. Anaplasia parameters did not correlate well with tumor invasion of deeper tissues and mitotic counts. Six of 10 OSCC cases were in animals with heavily pigmented eyes. Immunoexpression of p53 and p16 was observed in 3 cases each. Bovine herpesvirus and papillomavirus were not detected by PCR.Conclusions:Our results indicate that OSCC occurrence is most likely multifactorial with genetic, phenotypic, and environmental influences contributing to the pathogenesis of the disease.

show abstract

Section: Introductionmentioning

confidence: 99%

Ocular squamous cell carcinoma in Holstein cows from the South of Brazil

Fornazari¹,

Kravetz²,

Kiupel³

et al. 2017

Vet World

View full text Add to dashboard Cite

show abstract

“…Effect of virus on telomerase activity in animals previously was reported [36,37] but there is no report about ENA virus and telomerase activity in goats. This study showed that ENA virus up-regulate telomerase activity of infected cells and serum level.…”

Section: Discussionmentioning

confidence: 98%

“…Similarly, some viruses modulate the telomerase activity in animal cells [36,37] . Result of the present study showed that telomerase activity is effected by the disease.…”

Section: Discussionmentioning

confidence: 99%

Keçilerin Enzootik Intranazal Tümörlerinde Telomeraz Enziminin Düzeyinin Belirlenmesi

Şahinduran¹,

Özmen²,

Kiyici³

2018

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

Enzootic intranasal adenocarcinoma (ENA) of goats are contagious tumoral disease that characterized occurrence of tumoral masses in nasal cavity of the animals. The etiological agent of the disease is retrovirus and the disease cause high economical loses in goat industry. Retroviruses, respectively inducing lung or nasal adenocarcinomas. The true economic impact of ENA is not known because affected animals are given insights from the herd before actual diagnosis, and suspected disease incidence is rarely reported. Telomerase regulates the proliferative capacity of cells and this enzyme has critical role in tumor progression. The aim of this study is to evaluate telomerase activity in serum and tumoral tissue in goat with ENA. For this aim 11 goat's serum were analyses for telomerase activity. Tumoral tissue sections of totally 14 death goats were examined by immunohistochemically for telomerase expression. In addition, 10 normal goat's serum and nasal tissues that belong slaughtered healthy animals used as control. This study showed that increased serum and tissue telomerase activity in goats with ENA comparing to controls. Also our results showed that telomerase activity becomes a useful prediction marker for tumor development in infected but clinically healthy goats in infected flocks.

show abstract

“…Increased telomerase activity has been detected in cells infected with a variety of herpesviruses (10,(27)(28)(29), however, none of these viruses harbours any of the telomerase components except GaHV-2 recombinant viruses demonstrated significantly higher relative telomerase activity that in the non-infected chicken. Our findings correlate with previously published data that showed vTR enhance telomerase activity compared to the cellular TR (9,14).…”

Section: Discussionmentioning

confidence: 99%

Role of DNA Methylation and CpG Sites in the Viral Telomerase RNA Promoter during Gallid Herpesvirus 2 Pathogenesis

Pejaković

Mfossa

Wiggers

et al. 2020

J Virol

View full text Add to dashboard Cite

Gallid herpesvirus type 2 (GaHV-2) is an oncogenic alphaherpesvirus that induces malignant T-cell lymphoma in chicken. GaHV-2 encodes a viral telomerase RNA subunit (vTR) that plays a crucial role in virus-induced tumorigenesis, enhances telomerase activity and possesses function independent of the telomerase complex. vTR is driven by a robust viral promoter, highly expressed in virus-infected cells and regulated by two c-Myc response elements (c-Myc REs). The regulatory mechanisms involved in controlling vTR and other genes during viral replication and latency remain poorly understood but are crucial to understand this oncogenic herpesvirus. Therefore, we investigated DNA methylation patterns of CpG dinucleotides found in the vTR promoter and measured the impact of methylation on the telomerase activity. We demonstrated that telomerase activity was considerably increased following viral reactivation. Furthermore, CpG sites within c-Myc REs showed specific changes in methylation after in vitro reactivation and in infected animals over time. Promoter reporter assays indicated that one of the c-Myc RE is involved in regulating vTR transcription, and that methylation strongly influenced vTR promoter activity. To study the importance of the CpG sites found in c-Myc REs in virus-induced tumorigenesis, we generated a recombinant virus containing mutations in both CpG sites of c-Myc REs as well as revertant, by two-step Red-mediated mutagenesis. Introduced mutation in vTR promoter did not affect the replication properties of the recombinant viruses in vitro. In contrast, replication of the mutant virus in infected chickens was severely impaired, and tumour formation completely abrogated. Our data provided a more in-depth characterisation of c-Myc oncoprotein REs and DNA methylation involvement in transcriptional regulation of vTR. IMPORTANCE Previous studies demonstrated that telomerase RNAs possess functions that promote tumour development independent of the telomerase complex. vTR is a herpesvirus-encoded telomerase RNA subunit that plays a crucial role in virus-induced tumorigenesis and is expressed by a robust viral promoter that is highly regulated by the c-Myc oncoprotein binding to the E-boxes. Here we demonstrated that the DNA methylation patterns in the functional c-Myc response elements of the vTR promoter change upon reactivation from latency and that demethylation strongly increases telomerase activity in virus-infected cells. Moreover, the introduction of mutation in the CpG dinucleotides of the c-Myc binding sites resulted in decreased vTR expression and complete abrogation of tumour formation. Our study provides further confirmation of the involvement of specific DNA methylation patterns in the regulation of vTR expression and vTR importance for virus-induced tumorigenesis.

show abstract

Bovine herpesvirus type 1 (BHV-1) up-regulates telomerase activity in MDBK cells

Cited by 9 publications

References 27 publications

Ocular squamous cell carcinoma in Holstein cows from the South of Brazil

Ocular squamous cell carcinoma in Holstein cows from the South of Brazil

Keçilerin Enzootik Intranazal Tümörlerinde Telomeraz Enziminin Düzeyinin Belirlenmesi

Role of DNA Methylation and CpG Sites in the Viral Telomerase RNA Promoter during Gallid Herpesvirus 2 Pathogenesis

Contact Info

Product

Resources

About