Bottom-Up Community Proteome Analysis of Saliva Samples and Tongue Swabs by Data-Dependent Acquisition Nano LC-MS/MS Mass Spectrometry

Rabe, Alexander; Salazar, Manuela Gesell; Völker, Uwe

doi:10.1007/978-1-0716-1518-8_13

Cited by 2 publications

(4 citation statements)

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“…The obtained results are compared with the theoretical predictions available in the databases. The development of LC and MS/MS methods provides an increased ability to the identification of proteins and peptides from complex biological samples [ 84 , 102 ].…”

Section: Salivary Proteomementioning

confidence: 99%

Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics

2023

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The metabolomics and proteomics analysis of saliva, an excellent biofluid that is a rich source of biological compounds, allows for the safe and frequent screening of drugs, their metabolites, and molecular biomarkers of various diseases. One of the most frequently used analytical methods in saliva analysis is liquid chromatography coupled with mass spectrometry (LC-MS) and tandem mass spectrometry. The low ionisation efficiency of some compounds and a complex matrix makes their identification by MS difficult. Furthermore, quantitative analysis by LC-MS frequently cannot be performed without isotopically labelled standards, which usually have to be specially synthesised. This review presented reports on qualitative and quantitative approaches in salivary metabolomics and proteomics. The purpose of this manuscript was to present the challenges, advances, and future prospects of mass spectrometry, both in the analysis of salivary metabolites and proteins. The presented review should appeal to those interested in the recent advances and trends in qualitative and quantitative mass spectrometry in salivary metabolomics and proteomics, which may facilitate a diagnostic accuracy, the evaluation of treatment efficacy, the early diagnosis of disease, and a forensic investigation of some unapproved drugs for any medical or dietary administration.

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Section: Salivary Proteomementioning

confidence: 99%

Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics

2023

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“…The supernatant containing proteins was transferred to a new tube and stored on ice. The preparation of the protein mixture for the nLC-MS/MS measurement and the method for the mass spectrometric measurement were already described in detail [ 28 , 29 ]. Briefly, proteins were precipitated by TCA and washed several times with acetone.…”

Section: Methodsmentioning

confidence: 99%

“…We designed a workflow based on open-source software applications to analyze our metaproteomic datasets, described in detail in one of our earlier metaproteome studies [ 28 , 29 ]. Figure 2B provides an overview of the most important steps and is briefly described subsequently.…”

Section: Methodsmentioning

confidence: 99%

“…In this pilot study, we used an established metaproteomic approach [ 28 , 29 ] to evaluate the effect of the lozenges used in Welk et al [ 19 ] on the microbiome composition and the changes at the protein level with respect to their functions in metabolic pathways in the bacterial community. To the best of our knowledge, this is the first study combining a well-recognized and established clinical model in dentistry [ 20 ] with a metaproteomic study [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

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Impact of different oral treatments on the composition of the supragingival plaque microbiome

Rabe

Salazar

Michalik

et al. 2022

Journal of Oral Microbiology

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Background Dental plaque consists of a diverse microbial community embedded in a complex structure of exopolysaccharides. Dental biofilms form a natural barrier against pathogens but lead to oral diseases in a dysbiotic state. Objective Using a metaproteome approach combined with a standard plaque-regrowth study, this pilot study examined the impact of different concentrations of lactoperoxidase (LPO) on early plaque formation, and active biological processes. Design Sixteen orally healthy subjects received four local treatments as a randomized single-blind study based on a cross-over design. Two lozenges containing components of the LPO-system in different concentrations were compared to a placebo and Listerine®. The newly formed dental plaque was analyzed by mass spectrometry (nLC-MS/MS). Results On average 1,916 metaproteins per sample were identified, which could be assigned to 116 genera and 1,316 protein functions. Listerine® reduced the number of metaproteins and their relative abundance, confirming the plaque inhibiting effect. The LPO-lozenges triggered mainly higher metaprotein abundances of early and secondary colonizers as well as bacteria associated with dental health but also periodontitis. Functional information indicated plaque biofilm growth. Conclusion In conclusion, the mechanisms on plaque biofilm formation of Listerine® and the LPO-system containing lozenges are different. In contrast to Listerine®, the lozenges led to a higher bacterial diversity.

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Bottom-Up Community Proteome Analysis of Saliva Samples and Tongue Swabs by Data-Dependent Acquisition Nano LC-MS/MS Mass Spectrometry

Cited by 2 publications

References 40 publications

Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics

Qualitative and Quantitative Mass Spectrometry in Salivary Metabolomics and Proteomics

Impact of different oral treatments on the composition of the supragingival plaque microbiome

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