Botrytis fabae and Bean common mosaic virus (BCMV) are the most common diseases of Faba bean (Vicia faba L.) in TRNC

Ateş, Ayşegül Çolak; Fidan, Hakan; Yılmaz, Nihat; Konuksal, Ayda

doi:10.29278/azd.371067

Cited by 4 publications

(1 citation statement)

References 14 publications

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“…BCMV and BCMNV have previously been detected in various regions of Turkey (Açıkgöz, 1984;Güzel and Arlı Sökmen, 2003;Kutluk Yılmaz et al, 2002;Çulal Kılıç et al, 2015;Arlı Sökmen et al, 2016;Çolak Ateş et al, 2017). In this study, the occurrence of BCMNV in the collected seed samples in this region determined and DAS-ELISA data indicated that this virus is widely distributed in the selected seed samples of Lakes region.…”

Section: Conclusion and Recommendationssupporting

confidence: 58%

Göller Bölgesi Fasulye Üretim Alanlarında Fasulye Adi Mozayik ve Fasulye Adi Mozayik Nekroz Virüs’ü Enfeksiyonları

Kiliç¹,

Kök²,

Yardimci³

2020

European Journal of Science and Technology

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Common bean, belonging to family Leguminosae (Fabaceae), has an important place in people nutrition for thousands of years. Although many viruses infect bean plants, economically most important ones are potyviruses. Among them, BCMV (Bean common mosaic virus) and BCMNV (Bean common mosaic necrosis virus) are the most common viruses in common bean production areas all over the world. During 2014-2015 growing season, total of 361 bean samples (275 leaf and 86 seed) were collected from several localities in the Lakes Region. According to the results of DAS-ELISA (Double Antibody Sandwich Enzyme Linked Immunusorbent Assay) test, percentages of the infections of BCMV and BCMNV in the leaf samples were 26.18% and 15.27%, respectively. In seed samples, BCMV and BCMNV infection rates were 2.32% and 6.97%, respectively. Percentages of the mixed infections of the viruses in the leaf samples were 25.45%, while that in the seed samples were. 55.81%. In the mechanically inoculation studies where two isolates (BCMV-I and BCMN-BT) were used, typical symptoms of the viruses were observed. In molecular studies, a 850 bp fragment of the CP (Coat Protein) gene was amplified by IC-RT-PCR (Immunocapture Reverse Transcription Polimerase Chain Reaction) using BCMV specific primer pairs, but amplification of the CP region of RNA genome of BCMNV could not be succeeded. This is first report of biological and serological methods in Lakes region.

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Section: Conclusion and Recommendationssupporting

confidence: 58%

Göller Bölgesi Fasulye Üretim Alanlarında Fasulye Adi Mozayik ve Fasulye Adi Mozayik Nekroz Virüs’ü Enfeksiyonları

Kiliç¹,

Kök²,

Yardimci³

2020

European Journal of Science and Technology

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Bean Common Mosaic Potyvirus (BCMV) Characterized from Bean (Phaseolus vulgaris L.) Crops Affected by Mosaic Disease in Denizli Province, Türkiye

Usta

Güller

2023

Türkiye Tarımsal Araştırmalar Dergisi

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Bean (Phaseolus vulgaris) are one of the world's oldest crops with both financial and nutritional importance. Bean health is influenced by numerous biotic and abiotic factors, which can lead to significant crop losses. In the plant world, viruses are a challenging group of plant pathogens to combat, due to the difficulty of controlling them. BCMV is one of the prevalent viral agents that affects beans across the globe. Determining the presence of the agent in the relevant region is critical for minimizing crop losses by implementing appropriate preventive and control measures. In this study, 73 bean leaf samples were collected from bean growing areas in Denizli province of Turkey in 2022. The samples were screened for the presence of viral agents using Polymerase Chain Reaction (PCR) with specific primers targeting the polypeptide gene. 26 of the bean samples were found to be positive for BCMV. The coat protein gene sequences of two randomly selected positive isolates were sequenced and deposited in the GenBank with accession numbers OQ910196 and OQ910197. The nucleotide sequences of isolates were found to have high similarity with those of isolates identified in various regions of the world. Phylogenetic analysis indicated that these isolates from Denizli, Turkey were closely related to other Turkish isolates. However, since some Turkish isolates in the cluster associated with the Denizli isolates were identified as belonging to the US-5 or NL-6 strain, the current sequences may be related to these strains. Further research is necessary to identify the exact strain of the Denizli isolates, which could be achieved through the use of a strain differentiation set.

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Molecular Characterization of Polyprotein genes of Two BCMV (Bean common mosaic potyvirus) isolates in Antalya (Turkey) and Their Genomic Divergence

Usta

Güller

2020

Türk Tarım Ve Doğa Bilimleri Dergisi

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Common bean (Phaseolus vulgaris L.) is regarded as one of the most important crops of the Fabaceae family throughout the world. Diseases caused by viruses are the most important factor limiting the production of beans. Bean specimens with classic virus-like symptoms were collected from bean fields in Antalya (Turkey) in July and August, 2018. BCMV was examined by RT-PCR test (Reverse Transcriptase -Polymerase Chain Reaction) using appropriate primer pairs directed to the partial NIb and the capsid protein (CP) gene which was devised to identify and to characterize the viral agent. The PCR test produced approximately 850 bp amplicon of expected lengths in 11 out of 20 fresh leaf tissues, indicating the presence of BCMV. Two of them were randomly selected and molecularly cloned into a congruent plasmid vector to reveal the CP sequences of interested isolates. Obtained recombinant clones consisting of insert genes were bidirectionally sequenced and both of the sequences were registered in the GenBank with MN104839 and MN104840 accession number. The provided BCMV partial CP gene sequences comprised 823 bp coding for 274 amino acid residues. The CP gene of these isolates was aligned with those of 17 isolates deposited in the GenBank database from different geographical location and its phylogenetic relationships were determined. Molecular analysis of the CP gene sequences of Antalya isolates showed the highest identity rates between 91.22 % and 94.71 %, at the nucleotide level. Moreover, phylogenetic analyses revealed that BCMV-Antalya 1 and Antalya 10 are best clustered with the Turkish isolate (KT766179) and England isolate (AY112735), respectively. By this study, the genetic difference of BCMV isolates have been determined in the bean plant from Antalya province of Turkey.

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Botrytis fabae and Bean common mosaic virus (BCMV) are the most common diseases of Faba bean (Vicia faba L.) in TRNC

Cited by 4 publications

References 14 publications

Göller Bölgesi Fasulye Üretim Alanlarında Fasulye Adi Mozayik ve Fasulye Adi Mozayik Nekroz Virüs’ü Enfeksiyonları

Göller Bölgesi Fasulye Üretim Alanlarında Fasulye Adi Mozayik ve Fasulye Adi Mozayik Nekroz Virüs’ü Enfeksiyonları

Bean Common Mosaic Potyvirus (BCMV) Characterized from Bean (Phaseolus vulgaris L.) Crops Affected by Mosaic Disease in Denizli Province, Türkiye

Molecular Characterization of Polyprotein genes of Two BCMV (Bean common mosaic potyvirus) isolates in Antalya (Turkey) and Their Genomic Divergence

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