Book Review: Clinical Diagnosis and Management by Laboratory Methods

Henry, John B.

doi:10.1309/xl9u-4dyg-mj76-xcc8

Cited by 100 publications

(123 citation statements)

References 0 publications

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“…Consistent with this result, sedimentation velocity AUC experiments show that 2:1 FeTf⅐TfR complexes are the dominant species when HFE, Fe-Tf, and wtTfR are mixed such that the HFE and Fe-Tf are at higher concentrations than wtTfR. Therefore, soluble HFE is a poor competitor for binding to soluble TfR when Fe-Tf is present at high concentrations, including the micromolar concentrations corresponding to physiological levels of Fe-Tf in blood (37).…”

Section: Discussionsupporting

confidence: 56%

“…This assumption was verified by showing that HFE trafficks to Tf-positive endosomes in TfR-positive but not TfRnegative cells; thus, TfR binding is required for HFE to enter endosomes. By incubating the TfR-positive cells with Fe-Tf, we then demonstrated a loss of endosomal HFE-GFP fluorescence at relatively low concentrations of exogenous Fe-Tf (500 nM), with a substantial redistribution of GFP fluorescence to the cell surface at near physiological concentrations of exogenous Fe-Tf (micromolar) (37). Thus, external levels of Fe-Tf can influence both the localization and binding state of HFE in cells.…”

Section: Discussionmentioning

confidence: 88%

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HFE and Transferrin Directly Compete for Transferrin Receptor in Solution and at the Cell Surface

Giannetti¹,

Björkman

2004

Journal of Biological Chemistry

129

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Transferrin receptor (TfR) is a dimeric cell surface protein that binds both the serum iron transport protein transferrin (Fe-Tf) and HFE, the protein mutated in patients with the iron overload disorder hereditary hemochromatosis. HFE and Fe-Tf can bind simultaneously to TfR to form a ternary complex, but HFE binding to TfR lowers the apparent affinity of the Fe-Tf/TfR interaction. This apparent affinity reduction could result from direct competition between HFE and Fe-Tf for their overlapping binding sites on each TfR polypeptide chain, from negative cooperativity, or from a combination of both. To explore the mechanism of the affinity reduction, we constructed a heterodimeric TfR that contains mutations such that one TfR chain binds only HFE and the other binds only Fe-Tf. Binding studies using a heterodimeric form of soluble TfR demonstrate that TfR does not exhibit cooperativity in heterotropic ligand binding, suggesting that some or all of the effects of HFE on iron homeostasis result from competition with Fe-Tf for TfR binding. Experiments using transfected cell lines demonstrate a physiological role for this competition in altering HFE trafficking patterns.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 88%

HFE and Transferrin Directly Compete for Transferrin Receptor in Solution and at the Cell Surface

Giannetti¹,

Björkman

2004

Journal of Biological Chemistry

129

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“…Aspectos laboratoriais da medida da glicose plasmática A glicose, idealmente, deve ser medida em plasma livre de hemólise (17). Os anticoagulantes mais comuns (heparina, EDTA, citrato, oxalato) não interferem na dosagem.…”

Section: Classificaçãounclassified

Diabetes Melito: Diagnóstico, Classificação e Avaliação do Controle Glicêmico

Gross

Silveiro

Camargo

et al. 2002

Arq Bras Endocrinol Metab

159

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RESUMODiabetes e alterações da tolerância à glicose são freqüentes na população adulta e estão associados a um aumento da mortalidade por doença cardiovascular e complicações microvasculares. O diagnóstico destas situações deve ser feito precocemente, utilizando métodos sensíveis e acurados, já que mudanças no estilo de vida e a correção da hiperglicemia podem retardar o aparecimento do diabetes ou de suas complicações. O teste oral de tolerância à glicose é o método de referência, considerando-se a presença de diabetes ou tolerância à glicose diminuída quando a glicose plasmática de 2h após a ingestão de 75g de glicose for ≥ 200mg/dl ou ≥ 140 e <200mg/dl, respectivamente. Quando este teste não puder ser realizado, utiliza-se a medida da glicose plasmática em jejum, considerando-se como diabetes ou glicose alterada em jejum quando os valores forem ≥ 126mg/dl ou ≥ 110 e <126mg/dl, respectivamente. A medida da glico-hemoglobina não deve ser utilizada para o diagnóstico, mas é o método de referência para avaliar o grau de controle glicêmico a longo prazo. A classificação etiológica proposta atualmente para o diabetes melito inclui 4 categorias: diabetes melito tipo 1, diabetes melito tipo 2, outros tipos específicos de diabetes e diabetes gestacional. A classificação do paciente é usualmente feita em bases clínicas, mas a medida de auto-anticorpos e do peptídeo C pode ser útil em alguns casos. ABSTRACT Diabetes Mellitus: Diagnosis, Classification and Glucose Control Evaluation.Diabetes mellitus and other categories of impaired glucose tolerance are frequent in the adult population and are associated with an increased risk for cardiovascular disease and microvascular complications. The diagnosis of these entities should be performed early and using sensitive and accurate methods, since lifestyle changes and correction of hyperglycemia may delay the incidence of diabetes and its complications. Glucose tolerance test is the reference method and the diagnosis of diabetes and impaired glucose tolerance are established when the 2h plasma glucose after an oral intake of 75g of glucose is ≥ 200mg/dl or ≥ 140 and <200mg/dl, respectively. When it is not possible to perform this test, fasting plasma glucose levels ≥ 126mg/dl or ≥ 110 and <126mg/dl, respectively, are used to establish the diagnosis of diabetes and impaired fasting plasma glucose. Glycohemoglobin should not be used for the diagnosis but it is the reference method for evaluation of the long-term glucose control. The etiological classification of diabetes mellitus includes 4 categories: type 1 diabetes, type 2 diabetes, other specific types of diabetes and gestational diabetes. The assignment of the patient in each category usually is made on clinical grounds, however in some case the measurement of C-peptide and autoantibodies are necessary.

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“…Total cholesterol -Spectrophotometric [15] ii. LDL -Spectrophotometric [16] iii. HDL --Spectrophotometric [17] iv.…”

Section: Methods Of Assaymentioning

confidence: 99%