Bone turnover and oxidative stress markers in estrogendeficient rats treated with <i>Marantodes pumilum</i> leaves and roots: A comparative study

Abstract

Purpose: To investigate the relative changes in bone turnover and oxidative status markers of estrogen-deficient rats treated with Marantodes pumilum var. alata (MPva) leaf and root extracts. Methods: Thirty-six female Sprague-Dawley rats were divided into 6 groups (n = 6), namely: baseline (BL); placebo surgery (PS); ovariectomized control (OVXC); estrogen treatment (ERT); leaf extract treatment (MPv); and root extract treatment (MPr). Rats in all groups, except BL and PS, were ovariectomized to induce menopause. Through the oral route, ERT received 64.5 µg/kg/day estrogen (Premarin®); MPv received 20 mg/kg/day leaf extracts of MPva; MPr received 20 mg/kg/day root extract of MPva; while BL, OVXC, and PS served as untreated controls. At the end of 8 weeks treatment, blood and bone samples were collected for assay of bone turnover markers (osteocalcin and pyridinoline) and oxidative status markers (4-hydroxynonenal, superoxide dismutase, and glutathione peroxidase) using enzyme-linked immunosorbent assay (ELISA). Results: Significantly higher (p < 0.05) bone level of glutathione peroxidase was seen in MPv when compared with BL and OVXC. MPv also showed lower bone level of pyridinoline (p<0.05) compared to OVXC. Bone level of 4-hydroxynonenal in both MPv and MPr groups was significantly reduced (p < 0.05) when compared with OVXC. Conclusion: MPva leaf showed more remarkable effects on bone turnover and oxidative stress markers of ovariectomized rats than its roots and estrogen treatment. Thus, the use of MPva leaf, as an alternative to estrogen for treating postmenopausal osteoporosis, would be preferred to its roots.

Matrix metalloproteinase 9 a potential major player connecting atherosclerosis and osteoporosis in high fat diet fed rats

“…Estimation of serum levels of pyridinoline through the Competitive-ELISA principle [24]. Estimation of CTX tissue levels by ELISA (LifeSpan BioSciences, Washington, United States) according to the manufacturer's recommendations.…”

The cross-talk between matrix metalloproteinase-9, RANKL/OPG system and cardiovascular risk factors in ovariectomized rat model of postmenopausal osteoporosis

“…Serum IL-6 [ 19 ], TNF- alpha [ 20 ], alkaline phosphatase (ALP) [ 21 ], RANKL [ 22 ] and OPG [ 23 ] protein expression were estimated through sandwich ELISA technique. Serum levels of Osteocalcin (OC) [ 24 ] and pyridinoline [ 25 ] were estimated through the competitive enzyme immunoassay technique. Nitric oxide was determined in serum according to the method of Miranda et al [ 26 ].…”