Bone regeneration in rabbit sinus lifting associated with bovine BMP

Abstract

Autogenous bone is considered the optimal grafting material for sinus lifting, although its harvesting causes great patient discomfort. Various approaches have been taken in order to obtain sinus lifting with preexisting tissue. However, because of the unsuitability of such tissue, additional materials have been required. Alternatively, biomaterials from humans or other animals are used. In this study, the efficacy of using morphogenetic bovine bone protein (BMPb) to augment the maxillary sinus floor was examined. Four grafting materials were employed: lyophilized bovine bone powder, absorbable collagen flakes, natural hydroxylapatite, and synthetic hydroxylapatite. Two groups of rabbits were studied. In one group, graft material only was used. In the other, graft material was combined with 0.5 mg BMPb. During 8 weeks of observation, polyfluorochrome tracers were injected in subcutaneous tissue to evaluate new bone- deposition periods. Following sacrifice, the samples were examined under fluorescent and light microscopes. Results indicated 33.34% more newly formed bone in BMPb animals than in controls. Graft-material resorption increased, but natural HA showed no significant alterations. The results show that the use of BMPb, although providing osteoinduction, might not promote sufficient bone formation. Nonetheless, this material could provide an alternative to autogenous grafts, thereby avoiding patient discomfort.

Titanium implant osseointegration with calcium pyrophosphate in rabbits

“…The fluorochrome labels used in this study (alizarin, calcein, and tetracycline) are easily obtained with low cost. The colors detected for alizarin (tile red), calcein (green), and tetracycline (yellow) are in accordance with previous studies 28–30…”

Plasma rico em plaquetas associado ou não ao osso esponjoso autógeno no reparo de falhas ósseas experimentais

“…Já nas falhas tratadas com enxerto e com enxerto associado ao PRP, foi notado crescimento ósseo na porção central das falhas, diferentemente do preenchimento centrípeto dos grupos anteriores. Esse achado pode estar relacionado à presença do enxerto depositado no centro da falha, que promoveu osteogênese precoce e estimulou a formação óssea direta, conforme descreveram ALLEGRINI et al (2004).…”

Cell sheet engineering and other novel cell‐based approaches to periodontal regeneration

“…However, some side effects, such as tooth resorption, were also seen and thus recombinant human bone morphogenetic protein‐2 was not deemed to be applicable for use in periodontal therapy (14, 126). The combination of recombinant human bone morphogenetic protein‐2 on an absorbable collagen sponge carrier (recombinant human bone morphogenetic protein‐2/absorbable collagen sponge) is now commercially available as ‘InFuse® Bone Graft’ (Medtronic, Minneapolis, MN) and is applied for bone augmentation for sinus lifting and implant dentistry (2, 124).…”