Bone marrow mesenchymal stem cell-derived acellular matrix-coated chitosan/silk scaffolds for neural tissue regeneration

Xue, Cong; Ren, Haiyun; Zhu, Hui; Gu, Xiaokun; Guo, Qi; Zhou, Yi; Huang, Jiejie; Wang, Shengran; Zha, Guangbin; Gu, Jianhui; Yang, Yumin; Gu, Yun; Gu, Xiaosong

doi:10.1039/c6tb02959k

Cited by 38 publications

(19 citation statements)

References 49 publications

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“…Moreover, some researchers have pointed out that cell ingrowth depends not only on the size of apertures, but also on the degree of connectivity and the size of channels. There is an urgent need to systematically investigate the roles of porosity and pore size in osteogenic outcome, which will help us to design and fabricate orthopedic implants with the desired clinical performance [ 32 , 33 ].…”

Section: Effects Of Scaffold Structural Cues On Biological Responsmentioning

confidence: 99%

Scaffold Structural Microenvironmental Cues to Guide Tissue Regeneration in Bone Tissue Applications

Chen

Fan

Deng³

et al. 2018

Nanomaterials

140

130

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In the process of bone regeneration, new bone formation is largely affected by physico-chemical cues in the surrounding microenvironment. Tissue cells reside in a complex scaffold physiological microenvironment. The scaffold should provide certain circumstance full of structural cues to enhance multipotent mesenchymal stem cell (MSC) differentiation, osteoblast growth, extracellular matrix (ECM) deposition, and subsequent new bone formation. This article reviewed advances in fabrication technology that enable the creation of biomaterials with well-defined pore structure and surface topography, which can be sensed by host tissue cells (esp., stem cells) and subsequently determine cell fates during differentiation. Three important cues, including scaffold pore structure (i.e., porosity and pore size), grain size, and surface topography were studied. These findings improve our understanding of how the mechanism scaffold microenvironmental cues guide bone tissue regeneration.

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Section: Effects Of Scaffold Structural Cues On Biological Responsmentioning

confidence: 99%

Scaffold Structural Microenvironmental Cues to Guide Tissue Regeneration in Bone Tissue Applications

Chen

Fan

Deng³

et al. 2018

Nanomaterials

140

130

View full text Add to dashboard Cite

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“…Collagen prosthesis yielded significantly better results than the PLA-TMC prosthesis [11]. Repair outcomes of the treatment group were comparable to autograft repair [23].…”

Section: Introductionmentioning

confidence: 81%

“…Stem cells, for instance, skin-derived stem cells (SdSCs) in a collagen prosthesis, have shown the ability to improve peripheral nerve regeneration in terms of an increased number of myelinated axons [11]. Similarly, bone-marrow-derived stem cells (BMdSCs) are known to be helpful in successfully bridging nerve gaps by creating an adequate and favorable environment for the growth and myelination of regenerating axons, as well as the enhanced differentiation of BMdSCs into SCs [23]. In another study, the efficacy of the BMdSCs was assessed using a prosthesis filled with autologous BMdSCs, where axonal regeneration (myelinated axon number, the diameter of the regenerated fibers, and muscle weight), and functional recovery (amplitude and conduction velocity) were found to be comparable to autografting [17].…”

Section: Effectiveness Of Cell Therapymentioning

confidence: 99%

A Systematic Review of the Effectiveness of Cell-Based Therapy in Repairing Peripheral Nerve Gap Defects

2020

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Nerve prostheses are widely utilized to reconstruct segmental (gap) defects in peripheral nerves as an alternative to nerve grafting. However, with increasing gap length, the effectiveness of a nerve prosthesis becomes sub-optimal, which subsequently has made repairing larger gaps in peripheral nerves a significant challenge in the field of regenerative medicine. Recently, the structure of nerve prostheses has been significantly revised, which interestingly, has provided a promising avenue for the housing and proliferation of supportive cells. In this systematic review, cell implantation in synthetic nerve prostheses to enhance the regenerative capability of an injured nerve with a focus on identifying the cell type and mode of cell delivery is discussed. Of interest are the studies employing supportive cells to bridge gaps greater than 10 mm without the aid of nerve growth factors. The results have shown that cell therapy in conjunction with nerve prostheses becomes inevitable and has dramatically boosted the ability of these prostheses to maintain sustainable nerve regeneration across larger gaps and helped to attain functional recovery, which is the ultimate goal. The statistical analysis supports the use of differentiated bone-marrow-derived mesenchymal stem cells suspended in oxygen-carrying hydrogels in chitosan prostheses for bridging gaps of up to 40 mm; however, based on the imperfect repair outcomes, nerve grafting should not yet be replaced altogether.

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“…Chitosan is a polysaccharide made of d -glucosamine and N -acetyl- d -glucosamine linked by β(1,4) glycosidic bonds α(1–4)-2-amino-2-deoxyβ- d -glucan. It has been widely studied for applications in tissue regeneration as a result of its promising properties [ 42 , 43 , 44 , 45 ]. The polymer is a deacetylated form of chitin which is mostly obtained from the deacetylation of chitin extracted from crustaceous shells, but chitosan produced by mushrooms is also available on the market.…”

Section: Scaffolds For Bone Tissue Regenerationmentioning

confidence: 99%

Biodegradable Scaffolds for Bone Regeneration Combined with Drug-Delivery Systems in Osteomyelitis Therapy

et al. 2017

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A great deal of research is ongoing in the area of tissue engineering (TE) for bone regeneration. A possible improvement in restoring damaged tissues involves the loading of drugs such as proteins, genes, growth factors, antibiotics, and anti-inflammatory drugs into scaffolds for tissue regeneration. This mini-review is focused on the combination of the local delivery of antibiotic agents with bone regenerative therapy for the treatment of a severe bone infection such as osteomyelitis. The review includes a brief explanation of scaffolds for bone regeneration including scaffolds characteristics and types, a focus on severe bone infections (especially osteomyelitis and its treatment), and a literature review of local antibiotic delivery by the combination of scaffolds and drug-delivery systems. Some examples related to published studies on gentamicin sulfate-loaded drug-delivery systems combined with scaffolds are discussed, and future perspectives are highlighted.

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Bone marrow mesenchymal stem cell-derived acellular matrix-coated chitosan/silk scaffolds for neural tissue regeneration

Abstract: A novel tissue engineered nerve graft (TENG) was used for the first time to bridge a 60 mm long nerve gap in a dog sciatic nerve and achieved satisfactory results.

Cited by 38 publications

References 49 publications

Scaffold Structural Microenvironmental Cues to Guide Tissue Regeneration in Bone Tissue Applications

Scaffold Structural Microenvironmental Cues to Guide Tissue Regeneration in Bone Tissue Applications

A Systematic Review of the Effectiveness of Cell-Based Therapy in Repairing Peripheral Nerve Gap Defects

Biodegradable Scaffolds for Bone Regeneration Combined with Drug-Delivery Systems in Osteomyelitis Therapy

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