Bone Induction by Demineralized Dentin Matrix in Nude Mouse Muscles

Abstract

Purpose:This study examined the osteoinductive activity of demineralized human dentin matrix for nude mice.Methods:Twenty healthy nude mice weighing about 15 to 20 g were used for study. Demineralized human dentin matrix was prepared and implanted into the dorsal portion of nude mice (subcutaneous), which were sacrificed at two, four, and eight weeks after demineralized dentin matrix grafting and evaluated histologically by H&E and Masson trichrome staining. The specimens were also evaluated histomorphometrically.Results:The demineralized dentin matrix induced bone and cartilage formation independently in soft tissues. Histological examination showed bone-forming cells such as osteoblasts and fibroblasts at two, four, and eight weeks.Conclusion:These results suggest that demineralized human dentin matrix has osteoinductive ability, and is a good alternative to autogenous bone graft materials.

BMP-2 and type I collagen preservation in human deciduous teeth after demineralization

“…34 Interestingly, our data suggested that demineralized deciduous teeth contain much more BMP-2 with respect to native and demineralized dentin, 15 which are currently considered to be among the most suitable substitutes used for bone repair and regeneration. 25,[35][36][37] Such findings all strengthen the idea that BTs are very attractive bioactive materials for use in autologous bonegrafting procedures.…”

Demineralized Dentin Matrix as a Carrier of Recombinant Human Bone Morphogenetic Proteins: <i>in Vivo</i> Study

“…1). Dentin demineralization with 0.6 N HCl results in the elimination of the major part of the mineral phase and immunogenic components, while retaining a very low fraction of minerals (5-10 wt.%), the majority of type I collagen, and NCPs, providing an osteoconductive and osteoinductive scaffold containing several growth factors 35) (Fig. 1).…”

Demineralized Dentin and Enamel Matrices as Suitable Substrates for Bone Regeneration

“…In fact, D, in the form of native D (8, 9), and D derivatives, such as demineralized (10-11-12-13) and deproteinized D (14), have been used as graft materials in bone repair processes. Some experimental evidence from a critical literature survey highlighted the role of demineralized D matrix in promoting osteodifferentiation in vitro (13) and, even more interestingly, in increasing osteoinduction in vivo (15, 16). Taken together, these works point to the use of demineralized D matrices for the treatment of bone defects.…”