Demineralized Dentin and Enamel Matrices as Suitable Substrates for Bone Regeneration

Bono, Nina; Tarsini, Paolo; Candiani, Gabriele

doi:10.5301/jabfm.5000373

Cited by 37 publications

(61 citation statements)

References 32 publications

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“…The newly formed bone tissue will consist mainly of residues of the graft material and a small percentage of new bone. The use of tooth particulates, even after root canal treatment, offers the advantage of possessing osteoinductive molecules, like BMP-2 [16], that are present in the dentin. The use of the tooth graft is evaluated as an autologous graft, but some doubt remains about the use of teeth with root canal therapies.…”

Section: Discussionmentioning

confidence: 99%

“…The graft material, produced starting from the whole tooth, showed high wettability that allowed an easy handling and positioning on a host site [15]. A previous case series described the successful clinical outcomes of bone regeneration after autologous tooth grafting using this new device and demonstrated the complete filling of bone defects by hard tissue without any complications [16].…”

Section: Introductionmentioning

confidence: 99%

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Autologous Tooth Graft after Endodontical Treated Used for Socket Preservation: A Multicenter Clinical Study

et al. 2019

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The aim of the study was to evaluate the tooth extracted use as autologous tooth graft after endodontic root canal therapies used for socket preservation. To this purpose, the Tooth Transformer shredding and decontamination machine has been used. The graft obtained in this way, was inserted at the time of the extraction or at a second surgery altogether with the chosen regenerative therapy. This clinical trial enrolled patients with post-estractive defects requiring the restoration bone dimension and shape in the maxillary and mandibular zone. In addition, 98 patients with 119 extraction sockets were enrolled across 10 standardized centers. An innovative preparation method, using the dedicated automated device Tooth Transformer, able to transform autologous teeth in suitable grafting material, has been used. The extracted tooth was cleaned and treated using a Tooth Transformer and made a socket preservation. Thirteen Biopsies were realized to analyze the histologic outcomes at the average time of four months to demonstrate that the autologous tooth graft made from root after endodontic therapy should be used in human bone regeneration as graft for dental implant placement.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Autologous Tooth Graft after Endodontical Treated Used for Socket Preservation: A Multicenter Clinical Study

et al. 2019

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“…From these scientific considerations, and from several studies of dental tissue embryology, the first aim of research should be to verify if the extracted tooth, currently considered waste material [21][22][23], could be used as graft material in alveolar socket preservation procedures [24]. Furthermore, the behavior of the endodontically treated tooth in bone regeneration procedures should be determined, as well as if the tooth, properly cleaned after endodontic treatment, could be used in these surgical procedures [25,26].…”

Section: Discussionmentioning

confidence: 99%

Alveolar Socket Preservation with Different Autologous Graft Materials: Preliminary Results of a Multicenter Pilot Study in Human

Minetti¹,

Giacometti²,

Gambardella³

et al. 2020

Materials

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Background: The histological and histomorphometrical results were evaluated between vital whole and non-vital endodontically treated teeth used as autologous grafts in post-extractive socket preservation procedures. Methods: Twenty-eight patients (average age 51.79 ± 5.97 years) with post-extractive defects were enrolled in five dentistry centers. All patients were divided into two groups: with whole teeth (Group 1) and teeth with endodontical root canal therapy (Group 2). The extracted teeth were processed with the Tooth Transformer device to obtain a demineralized and granulated graft material used with a resorbable collagen membrane for socket preservation. After four months, 32 bone biopsies were obtained for histological, histomorphometric, and statistical analysis. Results: During the bone healing period, no infection signs were observed. Nineteen biopsies in group 1 and 13 biopsies in group 2 were detected. The histological analysis showed neither inflammatory nor infective reaction in both groups. Autologous grafts surrounded by new bone were observed in all samples and, at high magnification, partially resorbed dentin and enamel structures were detected. No gutta-percha or cement was identified. Small non-statistically significant differences between the groups, in total bone volume (BV), autologous graft residual, and vital bone percentage were detected. Conclusions: The study showed that the TT Transformer grafts were capable of producing new vital bone in socket preservation procedures. The histomorphometric results showed no statistical differences comparing whole and endodontically treated teeth in bone regeneration. Further studies will be carried out in order to understand the advantages of the autologous graft materials obtained from the tooth compared with the current biomaterials in bone regeneration treatments.

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“…2,3 They include metals, 4 ceramics, 5 as well as synthetic, 6,7 and natural polymers such as collagen and some composites. [8][9][10] In recent years, tooth [11][12][13] and tooth-derived materials 14,15 have come to the fore as very promising substrates for bone regeneration because they closely resemble bone tissue, in terms of matrix composition and topography. It is of note that, unlike bone autografts/allografts, which are rarely available at need, teeth are extracted every day and customarily discarded as waste.…”

Section: Introductionmentioning

confidence: 99%

“…16 Indeed, it is known that bone morphogenetic protein-2 (BMP-2), one of the most potentially effective osteoinductive proteins able to boost bone formation and repair in vitro, [17][18][19][20] is inherently present in, and released from, native dentin. Interestingly, since demineralization of highly mineralized tissues was found to increase BMP-2 bioavailability, 15 particular interest has been devoted to dentin in its demineralized form. 21,22 In this work we have attempted to take a step forward towards the use of tooth derivatives.…”

Section: Introductionmentioning

confidence: 99%

BMP-2 and type I collagen preservation in human deciduous teeth after demineralization

Bono

Tarsini

Candiani

2018

Journal of Applied Biomaterials & Functional Materials

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Background: Great interest has recently been focused on tooth and tooth derivatives as suitable substrates for the treatment of alveolar bone defects. Here, we propose the use of demineralized baby teeth (BT) as potential grafting materials for bone augmentation procedures. Methods: Particles of human BT (Ø < 1 mm) were demineralized by means of a chemical/thermal treatment. Demineralized BT particles were thoroughly characterized by scanning electron microscopy/energy dispersive X-ray analyses to evaluate the effects of the demineralization on BT topography and mineral phase composition, and by enzyme-linked immunosorbent assays (ELISA) to quantify collagen and bone morphogenetic protein-2 (BMP-2) protein contents. The response of SAOS-2 cells to exogenous BMP-2 stimulation was evaluated to identify the minimum BMP-2 concentration able to induce osteodifferentiation in vitro (alkaline phosphatase (ALP) activity). Results: The demineralization treatment led to a dramatic decrease in relative Ca and P content (%) of ≈75% with respect to the native BT particles, while preserving native protein conformation and activity. Interestingly, the demineralization process led to a rise in the bioavailability of BMP-2 in BT particles, as compared to the untreated counterparts. The BMP-2 content found in demineralized BT was also proved to be very effective in enhancing ALP activity, thus in the osteodifferentiation of SAOS-2 cells in vitro, as confirmed by cell experiments performed upon exogenously added BMP-2. Conclusions: In this study we demonstrate that the BMP-2 content found in demineralized BT is very effective in inducing cell osteodifferentiation, and strengthens the idea that BTs are very attractive bioactive materials for bonegrafting procedures.

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Demineralized Dentin and Enamel Matrices as Suitable Substrates for Bone Regeneration

Cited by 37 publications

References 32 publications

Autologous Tooth Graft after Endodontical Treated Used for Socket Preservation: A Multicenter Clinical Study

Autologous Tooth Graft after Endodontical Treated Used for Socket Preservation: A Multicenter Clinical Study

Alveolar Socket Preservation with Different Autologous Graft Materials: Preliminary Results of a Multicenter Pilot Study in Human

BMP-2 and type I collagen preservation in human deciduous teeth after demineralization

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