Bone Augmentation Using Novel Unidirectional Porous Hydroxyapatite with Bone Morphogenetic Protein-2 on Rat Skull

Kashiwazaki, Haruhiko; Harada, Naoki; Akazawa, Toshiyuki; Kabir, Md. Arafat; Minamida, Yasuhito; Murata, Masaru

doi:10.2485/jhtb.22.337

Cited by 3 publications

(3 citation statements)

References 17 publications

(16 reference statements)

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“…Other investigators have reported that a scaffold made of HAp with a unidirectional porous structure was more useful than a spherical interconnected porous structure since it could be impregnated with more cells [ 32 ]. When recombinant human bone morphogenetic protein-2 was implanted in bony defects of the skull in mice, significantly more new bone formation was observed than when HAp with a spherical interconnected porous structure was used [ 33 ]. Finally, thermal stimulation was found to promote new bone formation in the blocks implanted in rat tibia [ 34 ].…”

Section: Animal Experiments For the Development Of Regenos ® ; Unidirectional Porous Hapmentioning

confidence: 99%

Unidirectional porous beta-tricalcium phosphate and hydroxyapatite artificial bone: a review of experimental evaluations and clinical applications

et al. 2021

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In Japan, where allograft bone transplantation is not widespread, prospects for artificial bones are very high. Therefore, artificial bones with various compositions, porous structures, and porosities have been developed and employed for clinical use. Both Affinos® and Regenos® (made of beta-tricalcium phosphate and hydroxyapatite, respectively) are artificial bones with a unique unidirectional porous structure, in which pores with a diameter suitable for tissue penetration (25–300 μm) are aligned in one direction. The unidirectional porous structure allows rapid penetration of blood deep into the materials by a capillary effect. In animal experiments, Affinos® showed well-balanced resorption and was replaced with the host’s own bone from an early stage after implantation and new bone formation and remodeling were observed in the cortical bone and medullary cavity. When implanted for clinical situation, resorption from an early stage and good replacement with the patient’s own bone were also observed. Regenos® has an internal osteon-like material and a vascular-like structure that is maintained within the pores even after long-term implantation, as noted in an animal experiment. When implanted for clinical situation, good osteoconductivity was observed from an early stage of implantation. In addition, the material was observed to be slowly absorbed over time in some cases. We have discussed the beneficial effects of combining teriparatide and platelet-rich plasma impregnation and the potential prospects of these artificial bones.

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Section: Animal Experiments For the Development Of Regenos ® ; Unidirectional Porous Hapmentioning

confidence: 99%

Unidirectional porous beta-tricalcium phosphate and hydroxyapatite artificial bone: a review of experimental evaluations and clinical applications

et al. 2021

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“…After checking the expression of NC marker as previously described 12) , iPS-NCLCs were cultured and maintained in DMEM containing 10% FBS and 50 U/ml and 50 µg/ml PenicillinStreptomycin on CorningTM PrimariaTM tissue culture dishes (Thermo Fisher Scientifi c) to induce MSCs. For iPS-NCLC-MSCs cultured on hydroxyapatite (HA), unidirectional porous HA scaffolds [18][19][20] (Kurarife, Kuraray Medical Inc., Tokyo, Japan) were coated with fi bronectin overnight at 4°C and washed with PBS 3 times. Dissociated iPS-NCLC-MSCs were seeded on the HA scaff olds and cultured in DMEM supplemented with 10% FBS for 2 days before osteoinduction.…”

Section: Cell Culture and Msc Inductionmentioning

confidence: 99%

Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells

Kikuchi

Masuda

Fujiwara

et al. 2018

J. Hard Tissue Biology.

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Induced pluripotent stem (iPS) cells represent a powerful source for cell-based tissue regeneration because they are patient-specific cells and can differentiate into specialized cell types. Previously, we have demonstrated the derivation of neural crest like cells from iPS cells (iPS-NCLCs), and these cells have the potential to diff erentiate into dental mesenchymal cells, which subsequently diff erentiate into odontoblasts and dental pulp cells. In this study, we show that iPS-NCLCs can diff erentiate into mesenchymal stem cells (iPS-NCLC-MSCs), which contribute to craniofacial bone regeneration. iPS-NCLCs were cultured in serum-containing media and diff erentiated into functional MSCs, as confi rmed by expression MSC markers and their ability to diff erentiate into osteoblasts, adipocytes, and chondrocytes in vitro. iPS-NCLC-MSCs were negative for markers of undiff erentiated iPS cells and did not develop into teratomas when transplanted to immunodefi cient mice. Further, iPS-NCLC-MSCs grew normally and diff erentiated into osteoblasts on hydroxyapatite scaff olds in vitro. To assess the potential of iPS-NCLC-MSCs to regenerate craniofacial bone in vivo, iPS-NCLC-MSCs were transplanted into critical-size calvarial defects in immunodefi cient mice for 8 weeks. Histological analysis revealed that iPS-NCLC-MSCs diff erentiated into osteoblasts and contributed to bone regeneration without tumor formation. These results indicate that iPS-NCLC-MSCs could be a potential candidate for cell-based craniofacial bone tissue repair and regeneration.

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“…Bioceramics can be prepared with the same chemical composition similar to that of the human bone [1,2] . Bioceramics are commonly used in the medical fields as dental and bone implants because they have the ability to form a direct bond with the living bone in the presence of human physiological environment and because they have the potency to produce biomimetic hydroxyapatite apatite layer on the bone surface [3] .…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization and formulation of sodium calcium silicate bioceramic for drug delivery applications

Reddy

Lakshmi

Dass

et al. 2016

Science and Engineering of Composite Materials

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Sodium calcium silicate (Na 2 CaSiO 4 ) is a bioactive silicate with Na 2 O, CaO and SiO 2 as its basic components, which is similar to that of the composition of bioactive glasses. In the present study, pure sodium calcium silicate was synthesized by rapid combustion technique, and the synthesized sample was characterized by powder X-ray diffraction to check the phase purity. The scaffolds were prepared by varying the ratio of sodium calcium silicate and polyvinyl alcohol, and the apatite-formation ability of the scaffolds was examined by soaking them in a simulated body fluid. The results revealed the formation of hydroxyapatite on the surface of the scaffold after 5 days, which is found to be rapid when compared with the bioactivity of the calcium silicates and calcium magnesium silicates. The scaffolds were also loaded with ciprofloxacin as a model drug and analyzed for its drug release profile using UV spectrophotometer. The release profile did not vary with the change in bioceramic-tobiopolymer ratio, and 60% of the drug was released in 10 days, which is within the appreciable range for a targeted drug delivery system. Moreover, the experimental and simulated values of the release kinetics were compared by applying the existing mathematical model.

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Bone Augmentation Using Novel Unidirectional Porous Hydroxyapatite with Bone Morphogenetic Protein-2 on Rat Skull

Cited by 3 publications

References 17 publications

Unidirectional porous beta-tricalcium phosphate and hydroxyapatite artificial bone: a review of experimental evaluations and clinical applications

Unidirectional porous beta-tricalcium phosphate and hydroxyapatite artificial bone: a review of experimental evaluations and clinical applications

Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells

Synthesis, characterization and formulation of sodium calcium silicate bioceramic for drug delivery applications

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