BMPRIB and BMPRII mRNA expression levels in goat ovarian follicles and the in vitro effects of BMP-15 on preantral follicle development

Abstract

This study evaluated the levels of bone morphogenetic protein receptors BMPRIB and BMPRII mRNA in goat follicles and the effects of bone morphogenetic protein-15 (BMP-15) on the in vitro development of cultured preantral follicles. Real-time polymerase chain reaction (PCR) was used to analyze the levels of BMPRIB and BMPRII mRNA in caprine preantral follicles and in small and large antral follicles. Preantral follicles (≥150 μm) were also isolated from goat ovaries and cultured for 18 days in α-MEM(+) supplemented with or without BMP-15 (10, 50, or 100 ng/ml). At the end of culture, some follicles were fixed for ultrastructural evaluation. Real-time PCR showed a reduction in BMPRII mRNA levels from the primary to secondary follicles. Higher levels of BMPRIB mRNA were observed in granulosa/theca cells from large antral follicles compared with small antral follicles. Moreover, BMPRII mRNA was expressed to a greater extent in cumulus-oocyte complexes from large antral follicles than in their respective granulosa/theca cells. In culture, 50 ng/ml BMP-15 positively influenced antral cavity formation and follicle growth after 18 days and also maintained follicular integrity. Thus, BMPRIB and BMPRII mRNAs are present in all follicular categories. BMP-15 (50 ng/ml) stimulates growth, antrum formation and the ultrastructural integrity of isolated caprine preantral follicles after 18 days of culture.

Effects of Growth Hormone on In Situ Culture of Bovine Preantral Follicles are Dose Dependent

“…Similar studies showed that Lima et al. () assessed the caprine isolated preantral follicles during 18 days of in vitro culture with BMP‐15 (50 ng/ml) and found stimulation in the growth, antrum formation and ultrastructural integrity. Magalhães et al.…”

A bone morphogenetic protein ligand and receptors in mud crab: A potential role in the ovarian development

“…In addition, Sp-BMPRIB and Sp-BMPRII had an abundant expression in ovary as well as Sp-BMP7. The higher expression of some BMP ligands and receptors in ovary has been reported in many mammals, such as goat (Lima et al, 2012), hamster (Lima et al, 2012) and swine (Zhao et al, 2014). Thus, the results of tissues distribution, especially the abundant expression of BMP system in ovarian development attracted our attention.…”

Effect of Different Culture Systems and 3, 5, 3'-Triiodothyronine/Follicle-Stimulating Hormone on Preantral Follicle Development in Mice

“…In vitro follicle culture is an effective tool to detect the mechanisms of interaction oocyte, granulosa cell and theca cell. Many culture systems of follicle have been developed in different species [2], [3], [4], [5], [6], [7]. Although the fertilizable oocytes have been developed from mouse primordial and early preantral follicles in vitro [8], [9], [10], the follicle culture system for assaying the mechanism of preantral follicular development is still unstable.…”