No abstract

Electrically charged dust is considered in the framework of Einstein-Maxwelldilaton gravity with a Lagrangian containing the interaction term P (χ)F µν F µν , where P (χ) is an arbitrary function of the dilaton scalar field χ , which can be normal or phantom. Without assumption of spatial symmetry, we show that static configurations exist for arbitrary functions g 00 = exp(2γ(x i )) ( i = 1, 2, 3 ) and χ = χ(γ) . If χ = const , the classical Majumdar-Papapetrou (MP) system is restored. We discuss solutions that represent black holes (BHs) and quasi-black holes (QBHs), deduce some general results and confirm them by examples. In particular, we analyze configurations with spherical and cylindrical symmetries. It turns out that cylindrical BHs and QBHs cannot exist without negative energy density somewhere in space. However, in general, BHs and QBHs can be phantom-free, that is, can exist with everywhere nonnegative energy densities of matter, scalar and electromagnetic fields.

We consider Einstein-Maxwell-dilaton gravity with charged dust and interaction of the form P (χ)Fµν F µν , where P (χ) is an arbitrary function of the dilaton field χ that can be normal or phantom. For any regular P (χ), static configurations are possible with arbitrary functions g00 = exp(2γ(x i )) (i = 1, 2, 3) and χ = χ(γ), without any assumption of spatial symmetry. The classical Majumdar-Papapetrou system is restored by putting χ = const. Among possible solutions are black-hole (BH) and quasi-black-hole (QBH) ones. Some general results on BH and QBH properties are deduced and confirmed by examples. It is found, in particular, that asymptotically flat BHs and QBHs can exist with positive energy densities of matter and both scalar and electromagnetic fields.

The objective of this study was to evaluate different concentrations of growth hormone (GH) on the development of bovine preantral follicles cultured included in the ovarian tissue (in situ) on the rates of morphologically normal, viable, primordial and developing follicles, as well as the oocyte and follicle diameter and ultrastructural analysis. Ovarian fragments collected from cows with no cross-breeds defined were cultured in situ for 1 and 7 days in minimal essential medium (α-MEM+) supplemented with different concentrations of recombinant human GH (0, 10, 25, 50 ng/ml). The ovarian fragments non-cultured (control) and cultured were processed for classic histology, mechanical isolation and electron transmission microscopy (MET). The parameters underwent anova (Tukey's and Dunnett's tests) and chi-square test (χ(2) ). After 7 days of culture, the treatment with 50 ng/ml GH showed no differences with fresh control (p > 0.05) and had greater effectiveness than in the 0, 10 and 25 ng/ml GH concentrations of the morphologically normal follicles. Regarding the primordial follicles, a reduction was observed in the 50 ng/ml GH concentration concomitant with the significant increase in developing follicles, differing from both the fresh control and the other GH concentrations tested. In addition, 50 ng/ml GH showed a larger follicle and oocyte diameter when compared to the other treatments cultured. Similar structures were ultrastructurally observed in the control group, 50 ng/ml GH. Follicles cultured in 10 ng/ml GH showed nuclear invagination, vacuoles and lesioned basal membrane. Hence, it is concluded that 50 ng/ml GH is the most effective concentration for the development of preantral follicles cultured in situ.

The aim of this study was to evaluate the effects of vitamin E associated with rapid thawing on cryopreserved goat semen. Two bucks were used and eight ejaculates per animal were collected using artificial vagina. Semen was diluted with the following treatments: BIOXCELL (control), BIOXCELL + Equex (sodium lauryl sulphate) and BIOXCELL + vitamin E 100 μM. Semen was packaged into 0.25 mL straws and cooled at 5°C for 1 hour. Freezing was performed in liquid nitrogen vapor (−155°C) during 15 minutes. Then, the straws were immersed in liquid nitrogen (−196°C). Straws were thawed at 38°C/60 seconds or at 60°C/7 seconds with immediate sperm analysis. Hypoosmotic swelling test was performed adding a 20 μL aliquot of thawed semen to 1 mL of hypoosmotic solution (100 mOsm·Kg−1) followed by incubation during 60 minutes in water bath (38°C). Vitamin E did not affect any studied parameters (P > 0.05). Nevertheless, defrosting rate of 60°C/7 seconds improved sperm membrane functional integrity (P < 0.05). Current knowledge about goat semen cryopreservation is not sufficient to ensure high post-thawing recovery rates; thus, this study brings important data about using antioxidants and different thawing rates on cryopreservation process.