BMP2‐mimicking peptide modified with E7 coupling to calcined bovine bone enhanced bone regeneration associating with activation of the Runx2/SP7 signaling axis

Abstract

Commercial bone substitute, such as calcined bovine bone (CBB), is currently extensively used as an alternative to autogenous bone. However, CBB lacks osteoinductivity and merely serves as a scaffold for native bone formation. To address this issue, we designed and prepared a heptaglutamate (E7)‐modified BMP2‐mimicking peptide (7E) and carried out a series of comprehensive physical characterizations and in vivo and in vitro studies to evaluate its role in the repair of cranial defects. The data elucidated that the amount of peptide anchoring to the bone graft materials was remarkably increased after modified with E7. Of note, 7E had a relatively stable and durable release, which promoted the osteogenic differentiation of rat derived bone marrow mesenchymal stem cells (BMSCs) and enhanced the bone regeneration of a rabbit calvarial defect by regulating the expression of the Runx2/SP7 axis. In summary, the composite biomaterials incorporating the E7‐modified BMP2‐mimicking peptide and CBB prepared in this study is a novel bone augmentation material with the merits of non‐immunotoxicity, convenience, and low cost. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:80–93, 2020.

Preparation of 3D Printing PLGA Scaffold with BMP‐9 and P‐15 Peptide Hydrogel and Its Application in the Treatment of Bone Defects in Rabbits

“…e differentiation of osteoblasts in RUNX2 deficient mice is completely inhibited, and periosteal osteogenesis and endochondral osteogenesis do not occur [23]. Sp7 (osteoblast-specific transcription factor osterix) belongs to the SP/xklf family, which is expressed specifically in developing bone tissue and is a necessary transcription factor in osteoblast differentiation and bone formation [24]. According to western blot results, the PLGA scaffold of peptide hydrogel promoted the proliferation and differentiation of rat BMSCs cells and osteogenic induction.…”

Zn–Sr-sintered true bone ceramics enhance bone repair and regeneration

“…In the past, this material has been used as a suitable carrier for delivering ions, biomolecules, and drugs. [14][15][16] TBC eliminates the original immunogenicity during high-temperature calcination, retaining the original natural three-dimensional (3D) pore structure and good mechanical strength while being more easily degradable than hydroxyapatite (HA). 17 The porous surface structure provides space for osteoblast anchoring and the inward growth of bone tissue, improving osseointegration and mechanical interlocking and preventing implant failure by improving mechanical interlocking at the bone/implant interface.…”