Covalent grafting of hyperbranched poly-L-lysine on Ti-based implants achieves dual functions of antibacteria and promoted osteointegration in vivo

Yang, Zhijian; Xi, Yue; Bai, Jing; Jiang, Zhiwei; Wang, Shuqin; Zhang, Haolan; Dai, Wei; Chao-zhen, Chen; Gou, Zhongru; Yang, Guoli; Gao, Changyou

doi:10.1016/j.biomaterials.2020.120534

Cited by 84 publications

(56 citation statements)

References 74 publications

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“… 43 Yang et al covalently immobilized hyperbranched poly-L-lysine polymers onto TNN substrates to enhance antibacterial and osteointegration abilities at the same time. 44 Shen et al reported that Mg/Zn-metal organic framework on TNN titanium possessed antibacterial and anti-inflammatory properties. 45 According to the above statement, TNN and TNT substrates are the common but wise choice for further functionalization with special nanostructure and excellent biocompatibility.…”

Section: Discussionmentioning

confidence: 99%

Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration

Yu¹,

Xu²,

Zhang³

et al. 2021

IJN

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Background and Purpose: Micro-/nano-tubes (TNTs) and micro-/nano-nets (TNNs) are the common and sensible choice in the first step of combined modifications of titanium surface for further functionalization in the purpose of extended indications and therapeutic effect. It is important to recognize the respective biologic reactions of these two substrates for guiding a biologically based first-step selection. Materials and Methods: TNTs were produced by anodic oxidation and TNNs were formed by alkali-heat treatment. The original selective laser melting (SLM) titanium surface was set as control. Surface characterization was evaluated by scanning electron microscopy, surface roughness, and water contact angle measurements. Osteoclastogenesis and osteogenesis were measured. MC3T3-E1 cells and RAW 264.7 cells were used for in vitro assay in terms of adhesion, proliferation, and differentiation. In vivo assessments were taken on Beagle dogs with micro-CT and histological analysis. Results: TNN and TNT groups performed decreased roughness and increased hydrophilicity compared with SLM group. For biological detections, the highest ALP activity and osteogenesis-related genes expression were observed in TNT group followed by TNN group (P <0.05). Interestingly, when it comes to the osteoclastogenesis, TNNs displayed lowest TRAP activity and osteoclastogenesis-related genes expression and TNTs were lower than SLM but higher than TNNs (P <0.05). BV/TV around implants was highest in TNT group after 4 weeks (P <0.05). HE, ALP and TRAP staining showed that osteogenic and osteoclastic activity around TNTs were both higher than TNNs (P <0.05). Conclusion:TNNs and TNTs have dual advantages in promotion of osteogenesis and inhibition of osteoclastogenesis. Furthermore, TNNs showed better capability in inhibiting osteoclast activity while TNTs facilitated stronger osteogenesis. Our results implied that TNT substrates would take advantage in early application after implantation, while diseases with inappropriate osteoclast activity would prefer TNN substrates, which will guide a biologically based first-step selection on combined modification for different clinical purposes.

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Section: Discussionmentioning

confidence: 99%

Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration

Yu¹,

Xu²,

Zhang³

et al. 2021

IJN

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“…Another method of surface immobilization is silanization of the Ti surface, immobilizing organic molecules by covalent stabilization ( Kucharíková et al, 2016 ). Yang et al immobilized hyperbranched poly- l -lysine (HBPL) polymers in Ti via silane coupling agents ( Yang et al, 2021 ). The poly- l -lysine was able to interfere with the integrity of bacterial membranes and produce ROS to damage DNA of bacterial, exhibiting efficient sterilization properties in vivo and in vitro .…”

Section: Prevention Of Aseptic Implant Looseningmentioning

confidence: 99%

Multifunctional Coatings of Titanium Implants Toward Promoting Osseointegration and Preventing Infection: Recent Developments

et al. 2021

Front. Bioeng. Biotechnol.

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Titanium and its alloys are dominant material for orthopedic/dental implants due to their stable chemical properties and good biocompatibility. However, aseptic loosening and peri-implant infection remain problems that may lead to implant removal eventually. The ideal orthopedic implant should possess both osteogenic and antibacterial properties and do proper assistance to in situ inflammatory cells for anti-microbe and tissue repair. Recent advances in surface modification have provided various strategies to procure the harmonious relationship between implant and its microenvironment. In this review, we provide an overview of the latest strategies to endow titanium implants with bio-function and anti-infection properties. We state the methods they use to preparing these efficient surfaces and offer further insight into the interaction between these devices and the local biological environment. Finally, we discuss the unmet needs and current challenges in the development of ideal materials for bone implantation.

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“…[165] It has been used as a non-toxic cross-linker in the construction of many biocompatible and biodegradable materials and it makes a difference in the performance of polymer electrolytes such as mechanical properties and thermostabilities. [166] Y. Lin et al studied Li-ion conductivity properties of "CS/KH560" polymer electrolytes as a function of LiTFSI doping concentration. [167] The considerable shift in the position of characteristic 29 Si NMR peak associated to KH560 evidenced for the strong crosslinking between glycosidic linkages, three -OH groups related to starch and -Si-(OCH 3 ) 3 associated to KH560.…”

Section: Cross-linkers and Nanoparticles Doped Starch Polymer Electrolyte Systemsmentioning

confidence: 99%

Review on Microstructural and Ion‐conductivity Properties of Biodegradable Starch‐Based Solid Polymer Electrolyte Membranes

2021

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Currently, scientific developments in both the academic and industrial research communities are giving much attention towards the development of green chemistry secondary ion batteries with safety attributes. Biodegradable starch polymer-based electrolyte systems doped with different inorganic salts are gaining much attention owing to their intriguing and non-toxic nature. In this context, several research approaches have been identified for the improvement of lithium-ion conductivities at ambient temperatures, thermal and mechanical properties of starch polymer-based electrolytes. The present review provides consolidated information on chronological developments in the fabrication of different types of starch-based electrolytes (i.e., pure, blend, plasticized, and nanocomposites) and discusses microstructural, thermal, mechanical, and lithium-ion conductivity properties given lithium-ion batteries. Among all types, ionic liquids doped starch-based electrolytes demonstrated appreciable room temperature Li-ion conductivities. In parallel, a comparative study on Na + , K + , NH 4 + , Ag + , and Mg 2+ -ions conductivities in starch-based electrolytes is presented to emphasize the further research scope on the development of novel secondary ion batteries in complementing with lithium-ion batteries.

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Covalent grafting of hyperbranched poly-L-lysine on Ti-based implants achieves dual functions of antibacteria and promoted osteointegration in vivo

Cited by 84 publications

References 74 publications

Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration

Different Cell and Tissue Behavior of Micro-/Nano-Tubes and Micro-/Nano-Nets Topographies on Selective Laser Melting Titanium to Enhance Osseointegration

Multifunctional Coatings of Titanium Implants Toward Promoting Osseointegration and Preventing Infection: Recent Developments

Review on Microstructural and Ion‐conductivity Properties of Biodegradable Starch‐Based Solid Polymer Electrolyte Membranes

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