BMD and Serum Intact Osteocalcin in Postmenopausal Osteoporosis Women

Jagtap, Vanita R.; Ganu, Jayashri V.; Nagane, Nitin

doi:10.1007/s12291-010-0074-2

Cited by 66 publications

(52 citation statements)

References 13 publications

(11 reference statements)

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“…In osteoporosis, pro-inflammatory cytokines, such as TNF-a, promote osteoclastogenesis and inhibit osteoblastogenesis. Jagtap et al (2011) reported that the mean serum level of osteocalcin was significantly elevated in patients with postmenopausal osteoporosis when compared with that in healthy controls. Moreover, it was reported in a previous study that a mixture containing R. coreanus Miquel and A. membranaceus Bunge extracts helped in the recovery of serum osteocalcin and TNF-a levels in OVX mice (Jung Koo et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Effect of Astragalus polysaccharides on ovariectomy-induced osteoporosis in mice

Huo¹,

Sun²

2016

Genet. Mol. Res.

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ABSTRACT. Postmenopausal osteoporosis, a common type of osteoporosis in women, has become a serious public health issue. Astragalus polysaccharides (APS), possessing various pharmacological activities, are the active ingredients of Radix Astragali. It can be advantageous in the treatment of postmenopausal osteoporosis. In the present study, we evaluated the potential therapeutic effects of APS on postmenopausal osteoporosis by using a mice model induced by ovariectomy (OVX). Forty-eight female 6-week-old outbred ICR mice were randomly divided into six groups (N = 8): Sham group, OVX group, 17 b-estradiol (E2, 0.1 mg/kg)-treated OVX group, and APS (at three doses: 100, 200, and 400 mg/kg)-treated OVX groups. The effect of APS on the bone mineral density (BMD) was determined using dualenergy X-ray absorptiometry. The serum levels of receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG), osteocalcin, and tumor necrosis factor (TNF)-a were measured using ELISA kits. The results revealed that APS exerted significant antiosteoporotic activity by increasing the BMD considerably in a dosedependent manner. APS treatment reduced the serum RANKL levels considerably and increased the serum OPG levels, thereby lowering the ratio of RANKL/OPG. Furthermore, APS also markedly reduced osteocalcin and TNF-a concentration in OVX-induced postmenopausal osteoporosis mice model. These results showed that APS exerts a protective effect on bone loss in OVX mice. The molecular mechanism underlying this effect be the reduction of bone resorption and inhibition of osteoclastogenesis. Our findings suggest that APS may be a potential strategy for the prevention and treatment of postmenopausal osteoporosis.

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Section: Discussionmentioning

confidence: 99%

Effect of Astragalus polysaccharides on ovariectomy-induced osteoporosis in mice

Huo¹,

Sun²

2016

Genet. Mol. Res.

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“…The lifetime risk for women to have an osteoporotic fracture is 30-40% worldwide (1), which has a notable social, physical and economic impact (2)(3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

Age-dependent variations of cancellous bone in response to ovariectomy in C57BL/6J mice

et al. 2018

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Abstract. The ovariectomized (OVX) mouse model has been widely accepted to be suitable for the study of postmenopausal osteoporosis. However, whether C57BL/6J mice, a commonly used genetic background mouse strain, is an appropriate model for postmenopausal osteoporosis remains controversial. The present study investigated the effect of the OVX model on alterations in bone density and microarchitecture in C57BL/6J female mice of different ages. C57BL/6J mice were divided into 8-, 12-and 16-week-old groups (OVX 8 , OVX 12 and OVX 16 ) from the beginning of OVX. At 8 weeks post-surgery, the mice were anesthetized and micro-computed tomography was used to analyze the bone density and microarchitecture. The results revealed that OVX-induced loss of cancellous bone was greatest in OVX 8 , moderate in OVX 12 , and only a weak bone loss was observed in the OVX 16 group when compared with the SHAM 16 control group. In addition, the effect of genetic backgrounds in response to the OVX model were examined. Several other strains of mice, including inbred (BALB/c) and outbred (ICR and Kunming), were used in the present study, all of which were subjected to OVX at 8 weeks of age. The present findings revealed that the highest rate of bone loss was detected in C57BL/6J female mice. In addition, treatment with estrogen (17β-estradiol, 30 µg/kg five times per week) led to a significant increase in bone density in C57BL/6J mice compared with the other strains of mice. Therefore, these results may provide novel insights into the age-and strain-associated effect of OVX on regulating turnover of bone in female mice. The present findings also suggest 8-week-old C57BL/6J mice as an animal model for postmenopausal osteoporosis and preclinical testing of potential therapies for this disease.

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“…In osteoporosis, generally there is a deficiency of calcium and phosphorus level and since osteocalcin is a calcium dependent biomarker and has a strong affinity with bone matrix (hydroxyapatite) responsible for mineralization of bone. Osteoporosis leads to decreased hydroxyapatite crystal formation and hence results in increase in serum osteocalcin levels [8,9].…”

Section: Introductionmentioning

confidence: 99%