Bloqueio vascular de hastes de gérberas cv. Patrizia

Antes, Rose Beatriz; Montero, Cândida Raquel Scherrer; Rieth, Sandra; Duarte, Valmir; Bender, Renar João

doi:10.5007/2175-7925.2009v22n2p1

Cited by 5 publications

(7 citation statements)

References 11 publications

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“…Similar results to this experiment were verified in the work by Antes et al (2009) with rose stems, in this case the reduction of absorption of preservative solutions was at 7 DPC. Spricigo et al (2012) verified absorption decrease of solutions of chrysanthemum stems (Dendranthema grandiflora Tzvelev.)…”

Section: Resultssupporting

confidence: 90%

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Pós-colheita de hastes florais de cártamo colhidas em diferentes épocas e submetidas a diferentes soluções conservantes

Menegaes

Lidório

Bellé

et al. 2019

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The conservation of the cut flower stems aims to prolong durability, maintain the quality and reduce the losses after harvest, providing a greater period of lifespan and commercialization. Thus, the objective of this work was to evaluate the quality and durability in post-harvest of fresh safflower (Carthamus tinctorius L.) flower stems harvested in different times and submitted to different preservative solutions. The experiment was conducted in entirely randomized design and, organized in 4x8 (four preservative solutions and eight harvest seasons) factorial scheme, with four repetitions, and each experimental unit consisting of five floral stems. The cultivation of floral stems of safflower occurred at Floriculture Sector and the harvest seasons of them were carried out in the beginning of flowering from the sowing performed in the first seasonal half: in winter, spring and summer of 2016, autumn, winter, spring and summer of 2017 and autumn of 2018. And, the preservative solutions were: distilled water (control); distilled water + sucrose 2%; distilled water + sodium hypochlorite 2% and distilled water + sucrose 2% + sodium hypochlorite 2%. The floral stems were evaluated in relation to quality notes, dehydration and absorption of preservative solutions. We observed that the floral stems of safflower presented shelf life in average of nine days, with absorption of solution in average of 0.021 mL day-1 g-1 of fresh mass and that the use of preservatives was not beneficial to conservation in post-harvest.

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Section: Resultssupporting

confidence: 90%

“…The absorption of preservative solution (ASC) of floral stems in post-storage was calculated with the methodology by Antes et al (2009) by the formula:…”

Section: Methodsmentioning

confidence: 99%

Pós-colheita de hastes florais de cártamo colhidas em diferentes épocas e submetidas a diferentes soluções conservantes

Menegaes

Lidório

Bellé

et al. 2019

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“…Critical point drying with CO 2 proved to be adequate in preservation of both the structures of the gerbera stems ( Figure 9) and the microorganisms and exopolysaccharides of bacteria present in the samples (Figure 10 and 11). This same result with the use of critical point drying with CO 2 is in agreement with published studies on stems of Acaccia holosericea (RATNAYAKE et al, 2012), gerberas (ANTES et al, 2009), and roses (LI et al, 2012). Although the method is not completely problem-free, for it may cause some significant contractions in tissues (BOZZOLA et al, 1999), it is an effective manner for viewing colonization by microorganisms when the objective is to observe whether there is occlusion of the transport vessels.…”

Section: Dryingsupporting

confidence: 78%

“…The microorganisms responsible for vascular occlusion include bacteria of the genus Pseudomonas and yeasts (ZAGORY e REID, 1986). ; VAN DOORN et al, 1995 Scanning electron microscopy (SEM) has been used in countless studies for observation of microbial proliferation in the transport vessels of cut flowers such as gerberas (ANTES et al, 2009) and roses (LI et al, 2012),and for observation of the effect of preservative solutions on the cells at the base of gerbera stems (DURIGAN et al, 2009). The scanning electron microscope is an instrument used for microstructural analysis of solid materials, and produces results that are easily interpreted.…”

Section: Introductionmentioning

confidence: 99%

Methods of preparing flower stem samples for scanning electron microscopy

Spricigo¹,

Trento²,

Bresolin³

et al. 2015

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Brazil has great capacity for expansion in the floriculture sector. Studies on postharvest cut flowers contribute to development of the sector, helping to maintain the quality of domestic production. Scanning electron microscopy (SEM) is a powerful tool that allows viewing of flower structures and also microorganisms. The aim of this study was to evaluate methods of preparing flower stem samples for viewing in SEM as a support for studies on postharvest cut flowers. Ways of cutting, fixing, and drying samples were tested. Cutting with a stainless steel blade and through freeze-fracture were tested; fixation was carried out without the use of osmium tetroxide (OsO4); and drying of the samples was performed through freeze-drying and through critical point dryingwithCO2. Cutting with a stainless steel blade proved to be a satisfactory method for stem samples, with low cost and simple application compared to freeze-fracturing. Good fixation and high image contrast were obtained without the use of osmium tetroxide, thus avoiding the use of this toxic compound. Freeze-drying allowed the structure and morphological composition to be viewed, while critical point drying withCO2 preserved the microorganisms present in the samples.Keywords: gerbera,stainless steel blade, freeze-fracturing, freeze-drying, critical point with CO 2 . RESUMOMétodos de preparação de amostras de hastes florais para microscopia eletrônica de varredura O Brasil possui grande capacidade de expansão na área da floricultura. Estudos em pós-colheita de flores de corte contribuem para o desenvolvimento do setor, auxiliando na manutenção da qualidade da produção nacional. A microscopia eletrônica de varredura (MEV) é uma ferramenta potente que permite a visualização das estruturas florais e também de microrganismos. Este trabalho objetivou avaliar métodos de preparação de amostras de hastes florais para visualização de imagens em MEV como suporte em estudos de pós-colheita de flores cortadas. Foram testadas formas de obtenção de corte, fixação e secagem das amostras. Os tipos de cortes testados foram por meio de lâmina de aço inoxidável e criofratura, a fixação foi realizada sem a utilização do tetróxido de ósmio (OsO 4 ) e a secagem das amostras foi realizada por liofilização e por ponto crítico de CO 2 . O corte com a lâmina de aço inoxidável, demonstrou ser um método satisfatório para amostragem das hastes, de baixo custo e simples aplicação com relação a criofratura. Obteve-se uma boa fixação e alto contraste das imagens sem o uso do tetróxido de ósmio, desta forma, evitou-se a utilização deste composto tóxico. A secagem por liofilização permitiu a visualização da estrutura e composição morfológica, enquanto a secagem por ponto crítico de CO2 conservou os microorganismos presentes nas amostras. Palavras-chave: gérbera, lâmina de aço inoxidável, criofratura, liofilização, ponto crítico de CO 2 .(1) Trabalho recebido para publicação em 26

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“…Carbohydrates are the main sources of carbon and energy for the maintenance of all biochemical and physiological processes of flowers after cutting (Antes et al, 2009). In this study the carbohydrate content was significantly (p<0.05) affected by the solutions with storage time (Fig.…”

Section: Exogenous Application Of Growth Regulators Such As Gibberellins (Gibberellic Acid) Reduce the Damaging Action Of The Endogenous/mentioning

confidence: 53%

Maintenance solutions for the conservation of sunflower inflorescences

Sanches¹,

Moreira

Cordeiro

2019

Emir J Food Agric

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The sunflower (Helianthus annuus L.) is an ornamental plant of great acceptance in the market, however, as cut flower has its limited useful life, about 3 to 5 days at room temperature. In order to prolong shelf life and preserve post-harvest quality was evaluated the effect of maintenance solutions on sunflower cv. Oasis for 10 days storage at 25 °C. O experimento foi conduzido em delineamento inteiramente casualizado em arranjo fatorial 5x6, correspondendo a cinco tratamentos/soluções de manutenção (distilled water (control), sucrose, citric acid, gibberellic acid and benzylaminopurine to 5%) and six evaluation times (0, 2, 4, 6, 8 and 10 days). The effect of treatments on fresh mass, inflorescence turgescence, stem curvature, relative petal water content, respiratory rate and carbohydrate content was investigated. The results showed that the solutions with sucrose and gibberellic acid significantly (p<0.05) preserved the quality of the inflorescences as a function of the control of respiratory activity and water absorption, thereby reducing the physiological processes related to senescence. It is recommended to use sucrose 5% as a maintenance solution due to the low cost to producers and consumers.

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Bloqueio vascular de hastes de gérberas cv. Patrizia

Cited by 5 publications

References 11 publications

Pós-colheita de hastes florais de cártamo colhidas em diferentes épocas e submetidas a diferentes soluções conservantes

Pós-colheita de hastes florais de cártamo colhidas em diferentes épocas e submetidas a diferentes soluções conservantes

Methods of preparing flower stem samples for scanning electron microscopy

Maintenance solutions for the conservation of sunflower inflorescences

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