Blood Group Antigen Recognition via the Group A Streptococcal M Protein Mediates Host Colonization

Oliveira, David M. P. De; Hartley-Tassell, Lauren E.; Everest‐Dass, Arun; Day, Christopher J.; Dabbs, Rebecca A.; Ve, Thomas; Kobe, Boštjan; Nizet, Victor; Packer, Nicolle H.; Walker, Mark J.; Jennings, Michael P.; Sanderson-Smith, Martina L.

doi:10.1128/mbio.02237-16

Cited by 24 publications

(41 citation statements)

References 49 publications

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“…M protein interactions with ABO(H) and Le antigen structures were examined using single-cycle kinetic surface plasmon resonance (SPR) on a series S nitrilotriacetic acid chip (Biacore AB, Uppsala, Sweden) using a Biacore T200 (GE Healthcare, Waukesha, WI, USA) at 25°C. Glycan analytes (Dextra Laboratories, Reading, United Kingdom) were diluted in PBS, and binding assays were performed at concentrations from 0 to 10 mM as previously described in De Oliveira et al (1).…”

Section: Surface Plasmon Resonancementioning

confidence: 99%

“…Variations in the blood group antigens and secretory profile of each donor were assessed via liquid chromatographyelectrospray ionization mass spectroscopy of released N-and Olinked glycans as previously described in De Oliveira et al (1).…”

Section: Mass Spectrometrymentioning

confidence: 99%

“…We have previously shown that blood group antigen recognition by M1 protein correlates with differences in attachment to epithelial cells with differing glycosylation profiles (1). Blood group and Le antigen structures can make up 45% of the total epithelial cell surface glycan profile (23), representing an initial setting for interaction during GAS colonization.…”

Section: M1 M3 and M12 Gas Preferentially Colonize Hbe Cells Expresmentioning

confidence: 99%

“…The first stage of bacterial colonization is the recognition of host epithelial surfaces and associated structures. It is well documented that bacteria use glycan-binding proteins (lectins) to mediate host cellsurface recognition (1)(2)(3)(4). Glycans are abundantly expressed in extracellular fluids and on human epithelia, and the presence or absence of select glycan structures has been shown to influence the susceptibility of the host to infection by several bacterial and viral pathogens (5-9).…”

mentioning

confidence: 99%

“…We have previously shown that the obligate human pathogen, Streptococcus pyogenes (Group A Streptococcus; GAS), binds A, B, and O(H) blood group antigen structures with varying affinity, correlating with higher levels of M1T1 GAS colonization of epithelial cells that express the O(H)-antigen phenotype (1). Though GAS can cause a range of severe human infections, noninvasive pharyngitis is the most common disease state, with over 616 million cases recorded each year (14,15).…”

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confidence: 99%

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Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells

et al. 2019

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Colonization of the oropharynx is the initial step in Group A Streptococcus (GAS) pharyngeal infection. We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures. Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression. Removal of linkage‐specific fucose, galactose, N‐acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile. Furthermore, N‐linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo. These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.—De Oliveira, D. M. P., Everest‐Dass, A., Hartley‐Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson‐Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells. FASEB J. 33, 10808–10818 (2019). http://www.fasebj.org

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Section: Surface Plasmon Resonancementioning

confidence: 99%

Section: Mass Spectrometrymentioning

confidence: 99%

Section: M1 M3 and M12 Gas Preferentially Colonize Hbe Cells Expresmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells

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Subversion of host immune responses by otopathogens during otitis media

Parrish

Soni

Mittal

2019

Journal of Leukocyte Biology

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Otitis media (OM) is one of the most common ear diseases affecting humans. Children are at greater risk and suffer most frequently from OM, which can cause serious deterioration in the quality of life. OM is generally classified into two main types: acute and chronic OM (AOM and COM). AOM is characterized by tympanic membrane swelling or otorrhea and is accompanied by signs or symptoms of ear infection. In COM, there is a tympanic membrane perforation and purulent discharge. The most common pathogens that cause AOM are Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis whereas Pseudomonas aeruginosa and Staphylococcus aureus are commonly associated with COM. Innate and adaptive immune responses provide protection against OM. However, pathogens employ a wide arsenal of weapons to evade potent immune responses and these mechanisms likely contribute to AOM and COM. Immunologic evasion is multifactorial, and involves damage to host mucociliary tract, genetic polymorphisms within otopathogens, the number and variety of different otopathogens in the nasopharynx as well as the interaction between the host's innate and adaptive immune responses. Otopathogens utilize host mucin production, phase variation, biofilm production, glycans, as well as neutrophil and eosinophilic extracellular traps to induce OM. The objective of this review article is to discuss our current understanding about the mechanisms through which otopathogens escape host immunity to induce OM. A better knowledge about the molecular mechanisms leading to subversion of host immune responses will provide novel clues to develop effective treatment modalities for OM.

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Investigation of Group A Streptococcal Interactions with Host Glycan Structures Using High-Throughput Techniques: Glycan Microarray Analysis Using Recombinant Protein and Whole Cells

Indraratna

Oliveira

Hartley-Tassell

et al. 2020

Methods in Molecular Biology

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Blood Group Antigen Recognition via the Group A Streptococcal M Protein Mediates Host Colonization

Cited by 24 publications

References 49 publications

Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells

Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells

Subversion of host immune responses by otopathogens during otitis media

Investigation of Group A Streptococcal Interactions with Host Glycan Structures Using High-Throughput Techniques: Glycan Microarray Analysis Using Recombinant Protein and Whole Cells

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