Blocking the Expression of Both Bone Sialoprotein (BSP) and Osteopontin (OPN) Impairs the Anabolic Action of PTH in Mouse Calvaria Bone

Bouleftour, Wafa; Bouet, Guénaëlle; Granito, Renata Neves; Thomas, Mireille; Linossier, Marie‐Thérèse; Vanden‐Bossche, Arnaud; Aubin, Jane E.; Lafage-Proust, Marie-Hélène; Vico, Laurence; Malaval, Luc

doi:10.1002/jcp.24772

Cited by 29 publications

(17 citation statements)

References 26 publications

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“…The impaired bone formation owing to inactivation of OSX results in perinatal lethality in mice [37]. ALP measurement is considered as an early marker of osteogenic differentiation whereas OPN and OCN usually reflect the endpoint of osteogenesis in vitro [38]. COL-I is broadly distributed in bone and dentin, which acts as a structural support and biological signal to surrounding cells [39].…”

Section: Discussionmentioning

confidence: 99%

Yunnan Baiyao Conditioned Medium Promotes the Odonto/Osteogenic Capacity of Stem Cells from Apical Papilla via Nuclear Factor Kappa B Signaling Pathway

Pang

Wang

et al. 2019

BioMed Research International

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Yunnan Baiyao is a traditional Chinese herbal remedy that has long been used for its characteristics of wound healing, bone regeneration, and anti-inflammation. However, the effects of Yunnan Baiyao on the odonto/osteogenic differentiation of stem cells from apical papilla (SCAPs) and the potential mechanisms remain unclear. The aim of this study was to investigate the odonto/osteogenic differentiation effects of Yunnan Baiyao on SCAPs and the underlying mechanisms involved. SCAPs were isolated and cocultured with Yunnan Baiyao conditioned media. The proliferation ability was determined by cell counting kit 8 and flow cytometry. The differentiation capacity and the involvement of NF-κB pathway were investigated by alkaline phosphatase assay, alizarin red staining, immunofluorescence assay, real-time RT-PCR, and western blot analyses. Yunnan Baiyao conditioned medium at the concentration of 50 μg/mL upregulated alkaline phosphatase activity, induced more mineralized nodules, and increased the expression of odonto/osteogenic genes/proteins (e.g., OCN/OCN, OPN/OPN, OSX/OSX, RUNX2/RUNX2, ALP/ALP, COL-I/COL-I, DMP1, DSP/DSPP) of SCAPs. In addition, the expression of cytoplasmic phos-IκBα, phos-P65, and nuclear P65 was significantly increased in Yunnan Baiyao conditioned medium treated SCAPs in a time-dependent manner. Conversely, the differentiation of Yunnan Baiyao conditioned medium treated SCAPs was obviously inhibited when these stem cells were cocultured with the specific NF-κB inhibitor BMS345541. Yunnan Baiyao can promote the odonto/osteogenic differentiation of SCAPs via the NF-κB signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Yunnan Baiyao Conditioned Medium Promotes the Odonto/Osteogenic Capacity of Stem Cells from Apical Papilla via Nuclear Factor Kappa B Signaling Pathway

Pang

Wang

et al. 2019

BioMed Research International

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“…Noteworthy, HA-LF system showed an important effect in the induction of Osterix and IBSP, well known markers of osteoblast differentiation and activity [ 34 – 36 ]. The relative expression of these genes was double compared to the expression of the same genes in HA and LF samples, indicating that HA-LF has a synergistic effect on OBs expression of the genes involved in differentiation and production of bone matrix.…”

Section: Discussionmentioning

confidence: 99%

Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis

et al. 2015

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Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis. In a previous study we observed that the coupling of LF and biomimetic hydroxyapatite nanocrystals (HA), a material well-known for its bioactivity and osteoconductive properties, leads to a combined effect in the induction of osteogenic differentiation of mesenchymal stem cells. On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis. Biomimetic HA nanocrystals were synthesized and functionalized with LF (HA-LF) and then pre-osteoblasts (MC3T3-E1) and monocyte/macrophage cells lines (RAW 264.7), using as osteoclastogenesis in vitro model, were cultured separately or in co-culture in presence of HA-LF. The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

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“…While there were no significant differences in the volume of calcified tissue formed, BSP expression was clearly reduced in osteoprogenitors from sclerotic subchondral bone. Studies in knockout mice have convincingly demonstrated that BSP, a member of the small integrin-binding ligand N-linked glycoproteins (SIBLING) extracellular matrix protein family, is a master regulator of mineralization in vitro and in vivo [ 25 ]. It has not been investigated whether BSP-null mice are prone to develop OA but primary cortical bone formation and mineralization are clearly altered [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Alterations of Subchondral Bone Progenitor Cells in Human Knee and Hip Osteoarthritis Lead to a Bone Sclerosis Phenotype

Bianco

Todorov

Čengić

et al. 2018

IJMS

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Subchondral bone tissue plays a key role in the initiation and progression of human and experimental osteoarthritis and has received considerable interest as a treatment target. Elevated bone turnover and remodeling leads to subchondral bone sclerosis that is characterized by an increase in bone material that is less mineralized. The aim of this study was to investigate whether perturbations in subchondral bone-resident progenitor cells might play a role in aberrant bone formation in osteoarthritis. Colony formation assays indicated similar clonogenicity of progenitor cells from non-sclerotic and sclerotic subchondral trabecular bone tissues of osteoarthritic knee and hip joints compared with controls from iliac crest bone. However, the osteogenic potential at the clonal level was approximately two-fold higher in osteoarthritis than controls. An osteogenic differentiation assay indicated an efficient induction of alkaline phosphatase activity but blunted in vitro matrix mineralization irrespective of the presence of sclerosis. Micro-computed tomography and histology demonstrated the formation of de novo calcified tissues by osteoblast-like cells in an ectopic implantation model. The expression of bone sialoprotein, a marker for osteoblast maturation and mineralization, was significantly less in sclerotic progenitor cells. Perturbation of resident progenitor cell function is associated with subchondral bone sclerosis and may be a treatment target for osteoarthritis.

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Blocking the Expression of Both Bone Sialoprotein (BSP) and Osteopontin (OPN) Impairs the Anabolic Action of PTH in Mouse Calvaria Bone

Cited by 29 publications

References 26 publications

Yunnan Baiyao Conditioned Medium Promotes the Odonto/Osteogenic Capacity of Stem Cells from Apical Papilla via Nuclear Factor Kappa B Signaling Pathway

Yunnan Baiyao Conditioned Medium Promotes the Odonto/Osteogenic Capacity of Stem Cells from Apical Papilla via Nuclear Factor Kappa B Signaling Pathway

Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis

Alterations of Subchondral Bone Progenitor Cells in Human Knee and Hip Osteoarthritis Lead to a Bone Sclerosis Phenotype

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