Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis

Montesi, Monica; Panseri, Silvia; Iafisco, Michele; Adamiano, Alessio; Tampieri, Anna

doi:10.1371/journal.pone.0132633

Cited by 23 publications

(25 citation statements)

References 50 publications

(47 reference statements)

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“…The in vitro study confirmed also that the Sr 2+ released from Sr-BCs exerts its inhibitory effect on the expression of the principal genes involved in OCLs activity (Oscar and CtsK) [ 51 – 53 ]. Although Itgβ3 mRNA level seems to follow the same trend of decreasing over the time of culture, no statistically difference was observed, probably due to the proved role of integrin β3 in the interaction with apatitic structure [ 54 ]. In fact, Itgβ3 is considered the principal receptor in osteoclasts mechanisms involved in adhesion to the extracellular matrix, motility and activity.…”

Section: Discussionmentioning

confidence: 99%

Sr-substituted bone cements direct mesenchymal stem cells, osteoblasts and osteoclasts fate

et al. 2017

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Strontium-substituted apatitic bone cements enriched with sodium alginate were developed as a potential modulator of bone cells fate. The biological impact of the bone cement were investigated in vitro through the study of the effect of the nanostructured apatitic composition and the doping of strontium on mesenchymal stem cells, pre-osteoblasts and osteoclasts behaviours. Up to 14 days of culture the bone cells viability, proliferation, morphology and gene expression profiles were evaluated. The results showed that different concentrations of strontium were able to evoke a cell-specific response, in fact an inductive effect on mesenchymal stem cells differentiation and pre-osteoblasts proliferation and an inhibitory effect on osteoclasts activity were observed. Moreover, the apatitic structure of the cements provided a biomimetic environment suitable for bone cells growth. Therefore, the combination of biological features of this bone cement makes it as promising biomaterials for tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Sr-substituted bone cements direct mesenchymal stem cells, osteoblasts and osteoclasts fate

et al. 2017

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“…Lactoferrin is a glycoprotein that has an anabolic effect on bone by promoting osteoblast proliferation and differentiation as well as decreasing osteoclast formation (Lorget et al, 2002 ; Zhang et al, 2014 ). By coupling it to hydroxyapatite nanocrystals, Montesi et al showed that these two compounds can synergistically act as a bone anabolic by increasing OSX and IBSP mRNA expression in osteoblasts, reducing osteoclast formation, and downregulating OSCAR and CTSK mRNA expression in co-cultures of MC3T3-E1 and RAW264.7 (Montesi et al, 2015 ).…”

Section: Modulators Of Remodellingmentioning

confidence: 99%

In vitro Models of Bone Remodelling and Associated Disorders

Owen

Reilly

2018

Front. Bioeng. Biotechnol.

150

128

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Disruption of bone remodelling by diseases such as osteoporosis results in an imbalance between bone formation by osteoblasts and resorption by osteoclasts. Research into these metabolic bone disorders is primarily performed in vivo; however, in the last decade there has been increased interest in generating in vitro models that can reduce or replace our reliance on animal testing. With recent advances in biomaterials and tissue engineering the feasibility of laboratory-based alternatives is growing; however, to date there are no established in vitro models of bone remodelling. In vivo, remodelling is performed by organised packets of osteoblasts and osteoclasts called bone multicellular units (BMUs). The key determinant of whether osteoclasts form and remodelling occurs is the ratio between RANKL, a cytokine which stimulates osteoclastogenesis, and OPG, its inhibitor. This review initially details the different circumstances, conditions, and factors which have been found to modulate the RANKL:OPG ratio, and fundamental factors to be considered if a robust in vitro model is to be developed. Following this, an examination of what has been achieved thus far in replicating remodelling in vitro using three-dimensional co-cultures is performed, before overviewing how such systems are already being utilised in the study of associated diseases, such as metastatic cancer and dental disorders. Finally, a discussion of the most important considerations to be incorporated going forward is presented. This details the need for the use of cells capable of endogenously producing the required cytokines, application of mechanical stimulation, and the presence of appropriate hormones in order to produce a robust model of bone remodelling.

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“…Moreover, HA in combination with other growth factors, proteins or polymers has been used for studying the influence on both inflammatory and osteogenic responses. Thus, different studies have been performed with Lf-HA functionalized nanocrystals [50,51] revealing this combination as a promising system with anti-inflammatory properties and increased osteogenic capacity. It has been proved that biomimetic HA nanocrystals surface-functionalized with Lf have antibacterial activity effective against Gram-positive and Gram-negative bacteria [52][53][54].…”

Section: Of 19mentioning

confidence: 99%

Macrophage in vitro Response on Hybrid Coatings Obtained by Matrix Assisted Pulsed Laser Evaporation

et al. 2019

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The improvement in the research area of the implant by surface functionalization when correlated with the biological response is of major interest in the biomedical field. Based on the fact that the inflammatory response is directly involved in the ultimate response of the implant within the body, it is essential to study the macrophage-material interactions. Within this context, we have investigated the composite material-macrophage cell interactions and the inflammatory response to these composites with amorphous hydroxyapatite (HA), Lactoferrin (Lf), and polyethylene glycol-polycaprolactone (PEG-PCL) copolymer. All materials are obtained by Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique and characterized by Atomic Force Microscopy and Scanning Electron Microscopy. Macrophage-differentiated THP-1 cells proliferation and metabolic activity were assessed by qualitative and quantitative methods. The secretion of tumor necrosis factor alpha (TNF-α) and interleukin 10 (IL-10) cytokine, in the presence and absence of the inflammatory stimuli (bacterial endotoxin; lipopolysaccharide (LPS)), was measured using an ELISA assay. Our results revealed that the cellular response depended on the physical-chemical characteristics of the coatings. Copolymer-HA-Lf coatings led to low level of pro-inflammatory TNF-α, the increased level of anti-inflammatory IL-10, and the polarization of THP-1 cells towards an M2 pro-reparative phenotype in the presence of LPS. These findings could have important potential for the development of composite coatings in implant applications.

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Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis

Cited by 23 publications

References 50 publications

Sr-substituted bone cements direct mesenchymal stem cells, osteoblasts and osteoclasts fate

Sr-substituted bone cements direct mesenchymal stem cells, osteoblasts and osteoclasts fate

In vitro Models of Bone Remodelling and Associated Disorders

Macrophage in vitro Response on Hybrid Coatings Obtained by Matrix Assisted Pulsed Laser Evaporation

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