Blocking of TRPV-1 in the parodontium relieves orthodontic pain by inhibiting the expression of TRPV-1 in the trigeminal ganglion during experimental tooth movement in rats

Gao, Yunan; Liu, Yingfei; Zhang, Zhichao; Qiao, Hu; Lü, Zhen; Zhong, Tianyu; Liu, Yong; Zhou, Hong

doi:10.1016/j.neulet.2016.06.007

Cited by 16 publications

(16 citation statements)

References 31 publications

(31 reference statements)

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“…In rats, placement of a coil spring between the maxillary first molar and incisors produces changes in grimace scale and facial grooming (Yang et al, 2009; Liao et al, 2014). These behaviors are inhibited by morphine (Liao et al, 2014; Gao et al, 2016), indicating that these behaviors are relevant to nociception. A recent study also suggested that measuring changes in biting force in rats provides a functional surrogate outcome for biting-induced pain by orthodontic force (Long et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…The role of TRPV1 in non-evoked pain upon the application of orthodontic force was also suggested. Pharmacological inhibition or knockdown of TRPV1 attenuates facial grooming or grimace scale induced by orthodontic force in rats (Gao et al, 2016; Guo et al, 2019a). However, it is not known if TRPV1 contributes to other modalities of orthodontic pain, such as bite-evoked pain.…”

Section: Introductionmentioning

confidence: 99%

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TRPV1 and TRPV1-Expressing Nociceptors Mediate Orofacial Pain Behaviors in a Mouse Model of Orthodontic Tooth Movement

et al. 2019

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Orthodontic force produces mechanical irritation and inflammation in the periodontium, which is inevitably accompanied by pain. Despite its prevalence, treatment of orthodontic pain is ineffective. Elucidating underlying neural mechanisms is critical to improving the management of orthodontic pain. We have assessed the contribution of transient receptor potential vanilloid subtype 1 (TRPV1) and the TRPV1-expressing subset of nociceptive afferents to pain behaviors induced by orthodontic force in mice. Microfocus X-ray computed tomography analysis showed that application of an orthodontic force of 10 g to the maxillary first molar produced reliable tooth movement in mice. Mouse grimace scale (MGS) was evaluated as an indication of non-evoked spontaneous pain and bite force (BF) was measured for assessing bite-evoked nocifensive behaviors. Orthodontic force increased MGS and decreased BF, both of which were interpreted as increased levels of pain. These behaviors peaked at 1d and returned near to the sham level at 7d. Retrograde labeling and immunohistochemical assays showed TRPV1-expressing peptidergic afferents are abundantly projected to the periodontium. Direct injection of resiniferatoxin into trigeminal ganglia (TG) decreased TRPV1-expressing afferents by half in the targeted region of TG. The chemical ablation of TRPV1-expressing afferents significantly attenuated orthodontic pain behaviors assessed by MGS and BF. Consistently, the knockout of TRPV1 also attenuated orthodontic force-induced changes in MGS and BF. These results suggest that TRPV1 and TRPV1-expressing trigeminal nociceptors constitute a primary pathway mediating orthodontic pain behaviors in mice. This model will be useful for mechanistic studies on orthodontic pain aimed at developing novel approaches for painless orthodontics.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

TRPV1 and TRPV1-Expressing Nociceptors Mediate Orofacial Pain Behaviors in a Mouse Model of Orthodontic Tooth Movement

et al. 2019

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“…TRPV1 is a polymodal TRP receptor channel that can be activated by noxious temperatures, changes in pH or voltage, and fatty acid amides and vanilloids. TRPV1 is considered to be an interesting nociceptive target for relieving pain, including orthodontic pain (26). A previous study has found TRPV1 in vivo 34: 2389-2399 (2020) mRNA and protein expression were elevated in the TG during orthodontic pain.…”

Section: Discussionmentioning

confidence: 99%

Interval Vibration Reduces Orthodontic Pain Via a Mechanism Involving Down-regulation of TRPV1 and CGRP

Thammanichanon

Kaewpitak

Binlateh

et al. 2020

In Vivo

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“…These nociceptive signals can activate TRPV1 and induce painful sensations 22,23 . TRPV1 expression in TG and periodontal tissues was increased following orthodontic tooth movement 24–26 . Moreover, blocking TRPV1 in periodontal tissues with its antagonist alleviated orthodontic pain 26 .…”

Section: Introductionmentioning

confidence: 99%

“…TRPV1 expression in TG and periodontal tissues was increased following orthodontic tooth movement 24–26 . Moreover, blocking TRPV1 in periodontal tissues with its antagonist alleviated orthodontic pain 26 . All of these findings suggest that TRPV1 plays an essential role in the modulation of orthodontic pain.…”

Section: Introductionmentioning

confidence: 99%

Transient receptor potential Vanilloid 1-based gene therapy alleviates orthodontic pain in rats

et al. 2019

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Orthodontic pain that is induced by tooth movement is an important sequela of orthodontic treatment and has a significant effect on patient quality of life. Studies have shown that the high expression of transient receptor potential vanilloid 1 (TRPV1) in trigeminal ganglions plays a vital role in the transmission and modulation of orofacial pain. However, little is known about the role of TRPV1 in orthodontic pain. In this study, male Sprague–Dawley rats were randomly assigned to six groups to study the role of TRPV1 in the modulation of tooth-movement pain. The expression levels of TRPV1 mRNA and protein were determined by real-time PCR and western blot, respectively. Moreover, pain levels were assessed using the rat grimace scale (RGS). The role of TRPV1 in modulating tooth-movement pain was examined by injecting a TRPV1 antagonist into the trigeminal ganglia of rats. A lentivirus containing a TRPV1 shRNA sequence was constructed and transduced into the rats’ trigeminal ganglia. The results showed that the expression levels of TRPV1 protein and mRNA were elevated following tooth-movement pain. Pain levels increased rapidly on the 1 st day, peaked on the 3 rd day and returned to baseline on the 14 th day. The TRPV1 antagonist significantly reduced tooth-movement pain. The lentivirus containing a TRPV1 shRNA sequence was able to inhibit the expression of TRPV1 and relieved tooth-movement pain. In conclusion, TRPV1-based gene therapy may be a treatment strategy for the relief of orthodontic pain.

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Blocking of TRPV-1 in the parodontium relieves orthodontic pain by inhibiting the expression of TRPV-1 in the trigeminal ganglion during experimental tooth movement in rats

Cited by 16 publications

References 31 publications

TRPV1 and TRPV1-Expressing Nociceptors Mediate Orofacial Pain Behaviors in a Mouse Model of Orthodontic Tooth Movement

TRPV1 and TRPV1-Expressing Nociceptors Mediate Orofacial Pain Behaviors in a Mouse Model of Orthodontic Tooth Movement

Interval Vibration Reduces Orthodontic Pain Via a Mechanism Involving Down-regulation of TRPV1 and CGRP

Transient receptor potential Vanilloid 1-based gene therapy alleviates orthodontic pain in rats

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