Black Rice Potential in HDL and LDL Profile in Sprague Dawley Rat with High Cholesterol Diet

Nurhidajah, Nurhidajah; Astuti, Rias Ning; Nurrahman,

doi:10.1088/1755-1315/292/1/012019

Cited by 7 publications

(6 citation statements)

References 6 publications

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“…In the beginning of this first set of animal experiments, it was observed that the plasma cholesterol concentration of the rats was 40 mg/dL, in agreement with the reference values described in the literature [ 48 ]. From Figure 8 , it can be seen that significant differences between groups, in terms of cholesterol values, were only observed for animals supplemented with extract and ezetimibe (EEB + Ezet) when compared to the control group, with only this group showing a decrease in cholesterol levels.…”

Section: Resultssupporting

confidence: 85%

Nanoformulation of Seaweed Eisenia bicyclis in Albumin Nanoparticles Targeting Cardiovascular Diseases: In Vitro and In Vivo Evaluation

et al. 2022

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Natural products, especially those derived from seaweeds, are starting to be seen as effective against various diseases, such as cardiovascular diseases (CVDs). This study aimed to design a novel oral formulation of bovine albumin serum nanoparticles (BSA NPs) loaded with an extract of Eisenia bicyclis and to validate its beneficial health effects, particularly targeting hypercholesterolemia and CVD prevention. Small and well-defined BSA NPs loaded with Eisenia bicyclis extract were successfully prepared exhibiting high encapsulation efficiency. Antioxidant activity and cholesterol biosynthesis enzyme 3-hydroxy-3 methylutaryl coenzyme A reductase (HMGR) inhibition, as well as reduction of cholesterol permeation in intestinal lining model cells, were assessed for the extract both in free and nanoformulated forms. The nanoformulation was more efficient than the free extract, particularly in terms of HMGR inhibition and cholesterol permeation reduction. In vitro cytotoxicity and in vivo assays in Wistar rats were performed to evaluate its safety and overall effects on metabolism. The results demonstrated that the Eisenia bicyclis extract and BSA NPs were not cytotoxic against human intestinal Caco-2 and liver HepG2 cells and were also safe after oral administration in the rat model. In addition, an innovative approach was adopted to compare the metabolomic profile of the serum from the animals involved in the in vivo assay, which showed the extract and nanoformulation’s impact on CVD-associated key metabolites. Altogether, these preliminary results revealed that the seaweed extract and the nanoformulation may constitute an alternative natural dosage form which is safe and simple to produce, capable of reducing cholesterol levels, and consequently helpful in preventing hypercholesterolemia, the main risk factor of CVDs.

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Section: Resultssupporting

confidence: 85%

Nanoformulation of Seaweed Eisenia bicyclis in Albumin Nanoparticles Targeting Cardiovascular Diseases: In Vitro and In Vivo Evaluation

et al. 2022

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“…Pangestika et al also reported that total cholesterol and LDL-C levels of hypercholesterolemic rats started to decrease at Day 21 after treatment with simvastatin and almost reaching back to a normal level at Day 42 [ 64 ]. It was also reported that statin also prevented the levels of total cholesterols and LDL-C from increasing towards the hypercholesteraemic conditions in rats fed with high fat diet [ 63 , 65 ]. In addition, statin also decreased the levels of triglycerides in dyslipidemic rats [ 66 ].…”

Section: Discussionmentioning

confidence: 99%

Acanthaster planci Inhibits PCSK9 and Lowers Cholesterol Levels in Rats

et al. 2021

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Atherosclerosis is the main cause of cardiovascular diseases which in turn, lead to the highest number of mortalities globally. This pathophysiological condition is developed due to a constant elevated level of plasma cholesterols. Statin is currently the widely used treatment in reducing the level of cholesterols, however, it may cause adverse side effects. Therefore, there is an urgent need to search for new alternative treatment. PCSK9 is an enzyme responsible in directing LDL-receptor (LDL-R)/LDL-cholesterols (LDL-C) complex to lysosomal degradation, preventing the receptor from recycling back to the surface of liver cells. Therefore, PCSK9 offers a potential target to search for small molecule inhibitors which inhibit the function of this enzyme. In this study, a marine invertebrate Acanthaster planci, was used to investigate its potential in inhibiting PCSK9 and lowering the levels of cholesterols. Cytotoxicity activity of A. planci on human liver HepG2 cells was carried out using the MTS assay. It was found that methanolic extract and fractions did not exhibit cytotoxicity effect on HepG2 cell line with IC50 values of more than 30 µg/mL. A compound deoxythymidine also did not exert any cytotoxicity activity with IC50 value of more than 4 µg/mL. Transient transfection and luciferase assay were conducted to determine the effects of A. planci on the transcriptional activity of PCSK9 promoter. Methanolic extract and Fraction 2 (EF2) produced the lowest reduction in PCSK9 promoter activity to 70 and 20% of control at 12.5 and 6.25 μg/mL, respectively. In addition, deoxythymidine also decreased PCSK9 promoter activity to the lowest level of 60% control at 3.13 μM. An in vivo study using Sprague Dawley rats demonstrated that 50 and 100 mg/kg of A. planci methanolic extract reduced the total cholesterols and LDL-C levels to almost similar levels of untreated controls. The level of serum glutamate oxalate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) showed that the administration of the extract did not produce any toxicity effect and cause any damage to rat liver. The results strongly indicate that A. planci produced a significant inhibitory activity on PCSK9 gene expression in HepG2 cells which may be responsible for inducing the uptake of cholesterols by liver, thus, reducing the circulating levels of total cholesterols and LDL-C. Interestingly, A. planci also did show any adverse hepato-cytotoxicity and toxic effects on liver. Thus, this study strongly suggests that A. planci has a vast potential to be further developed as a new class of therapeutic agent in lowering the blood cholesterols and reducing the progression of atherosclerosis.

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“…Kelainan lipid ini terjadi karena resistensi insulin mengganggu lipolisis trigliserida yang dilakukan oleh enzim lipase. Efek dari terganggunya lipolisis trigliserida mengakibatkan naiknya kadar lipid dalam darah (Nurhidajah et al, 2019).…”

Section: Pendahuluanunclassified

Pengaruh pemberian ekstrak bekatul beras hitam (Oryza sativa L. indica) terhadap kadar MDA, SOD dan trigliserida pada tikus diabetes mellitus tipe 2

Monikasari

Widyastiti

Mahati³

et al. 2023

Aceh. Nutri. J.

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In type 2 diabetes mellitus, the pancreas can produce the hormone insulin, but the body has difficulty using it. The study aimed to prove the effect of black rice bran extract on MDA, SOD, and triglyceride in type 2 Diabetic Mellitus rats. The method is quasi-experimental with a pre-posttest randomized control group design in 2021. The intervention was given for 21 days to 42 male Wistar rats. MDA examination using the TBARs method, SOD examination using a UV-Visible spectrophotometer. Triglyceride examination using a spectrophotometer. Statistical analysis used Paired T-Test or Wilcoxon test and One-Way ANOVA or Kruskal Wallis test. Descriptive research findings show that the intervention of black rice bran extract at a dose of 60 mg/200g reduced MDA in rats to 2,21 mmol/ml and triglycerides to 85,17 mg/dL. In addition, it can increase SOD to 69,67 units/ml. Almost the same as the intervention of metformin 9 mg/200 g body weight. However, statistically, no significant difference exists in the interventions carried out (p> 0,05). The study concluded a decrease in MDA, SOD, and triglyceride in rats, given the black rice bran extract intervention.

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Black Rice Potential in HDL and LDL Profile in Sprague Dawley Rat with High Cholesterol Diet

Cited by 7 publications

References 6 publications

Nanoformulation of Seaweed Eisenia bicyclis in Albumin Nanoparticles Targeting Cardiovascular Diseases: In Vitro and In Vivo Evaluation

Nanoformulation of Seaweed Eisenia bicyclis in Albumin Nanoparticles Targeting Cardiovascular Diseases: In Vitro and In Vivo Evaluation

Acanthaster planci Inhibits PCSK9 and Lowers Cholesterol Levels in Rats

Pengaruh pemberian ekstrak bekatul beras hitam (Oryza sativa L. indica) terhadap kadar MDA, SOD dan trigliserida pada tikus diabetes mellitus tipe 2

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