Purpose -Aims to present the ontology engineering methodology DILIGENT, a methodology focussing on the evolution of ontologies instead of the initial design, thus recognizing that knowledge is a tangible and moving target.Design/methodology/approach -First describes the methodology as a whole, then detailing one of the five main steps of DILIGENT. The second part describes case studies, either already performed or planned, and what we learned (or expect to learn) from them.Findings -With the case studies it was discovered the strengths and weaknesses of DILIGENT. During the evolution of ontologies, arguments need to be exchanged about the suggested changes. Identifies those kind of arguments which work best for the discussion of ontology changes.Research implications -DILIGENT recognizes ontology engineering methodologies like OnToKnowledge or Methontology as proven useful for the initial design, but expands them with its strong focus on the user-centric further development of the ontology and the provided integration of automatic agents in the process of ontology evolution.Practical implications -With DILIGENT the experience distilled from a number of case studies and this offers the knowledge manager a methodology to work in an ever-changing environment.Originality/value -DILIGENT is the first methodology to put focus not on the initial development of the ontology, but on the user and his usage of the ontology, and on the changes introduced by the user. We take the user's own view seriously and enable feedback towards the evolution of the ontology, stressing the ontology's role as a shared conceptualisation.

Abstract. Knowledge management solutions relying on central repositories sometimes have not met expectations, since users often create knowledge adhoc using their individual vocabulary and using their own decentral IT infrastructure (e.g., their laptop). To improve knowledge management for such decentralized and individualized knowledge work, it is necessary to, first, provide a corresponding IT infrastructure and to, second, deal with the harmonization of different vocabularies/ontologies. In this paper, we briefly sketch the technical peer-to-peer platform that we have built, but then we focus on the harmonization of the participating ontologies. Thereby, the objective of this harmonization is to avoid the worst incongruencies by having users share a core ontology that they can expand for local use at their will and individual needs. The task that then needs to be solved is one of distributed, loosely-controlled and evolving engineering of ontologies. We have performed along these lines. To support the ontology engineering process in the case study we have furthermore extended the existing ontology engineering environment, OntoEdit. The case study process and the extended tool are presented in this paper.

After performing hydrophilic interaction and weak anion exchange high-performance liquid chromatography to analyze N-glycans in the plasma of 1991 people, we identified several individuals that differed significantly from the "normal" profile of N-glycans. By performing consensus scoring of pairwise distances between vectors containing measured glycan values, we formed six groups of individuals with specific glyco-phenotypes. Some aberrations from the normal plasma protein patterns were found to be associated with clinical conditions (such as renal problems in people with increased monosialylated biantennary glycans, A2G2S1), while other substantial changes in N-glycan structure, such as the near complete absence of neutral glycans or antennary fucosylated tri- and tetraantennary glycans, were not associated with any observed adverse health outcomes. These results demonstrate the existence of specific altered glyco-phenotypes in some individuals and indicate that in some cases they might represent risk factors for the development of specific diseases.

Natural products, especially those derived from seaweeds, are starting to be seen as effective against various diseases, such as cardiovascular diseases (CVDs). This study aimed to design a novel oral formulation of bovine albumin serum nanoparticles (BSA NPs) loaded with an extract of Eisenia bicyclis and to validate its beneficial health effects, particularly targeting hypercholesterolemia and CVD prevention. Small and well-defined BSA NPs loaded with Eisenia bicyclis extract were successfully prepared exhibiting high encapsulation efficiency. Antioxidant activity and cholesterol biosynthesis enzyme 3-hydroxy-3 methylutaryl coenzyme A reductase (HMGR) inhibition, as well as reduction of cholesterol permeation in intestinal lining model cells, were assessed for the extract both in free and nanoformulated forms. The nanoformulation was more efficient than the free extract, particularly in terms of HMGR inhibition and cholesterol permeation reduction. In vitro cytotoxicity and in vivo assays in Wistar rats were performed to evaluate its safety and overall effects on metabolism. The results demonstrated that the Eisenia bicyclis extract and BSA NPs were not cytotoxic against human intestinal Caco-2 and liver HepG2 cells and were also safe after oral administration in the rat model. In addition, an innovative approach was adopted to compare the metabolomic profile of the serum from the animals involved in the in vivo assay, which showed the extract and nanoformulation’s impact on CVD-associated key metabolites. Altogether, these preliminary results revealed that the seaweed extract and the nanoformulation may constitute an alternative natural dosage form which is safe and simple to produce, capable of reducing cholesterol levels, and consequently helpful in preventing hypercholesterolemia, the main risk factor of CVDs.