Nanoformulation of Seaweed Eisenia bicyclis in Albumin Nanoparticles Targeting Cardiovascular Diseases: In Vitro and In Vivo Evaluation

Pinto, Sofia; Gaspar, Maria Manuela; Ascensão, Lia; Faísca, Pedro; Reis, Catarina Pinto; Pacheco, Rita

doi:10.3390/md20100608

Cited by 4 publications

(18 citation statements)

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“…The results of the phenolic quantification of the Arame, Nori, and Fucus extracts, as well as its MeOH and H 2 O fractions, are shown in Figure 1 . The extract showing higher TPC was Aramé, with 0.062 ± 0.005 mg PGE/mg dry mass [ 13 ]. However, the methanol fraction of Aramé showed the highest TPC per mg dry mass amongst all the analyzed samples.…”

Section: Resultsmentioning

confidence: 99%

“…While Fucus vesiculosus was collected from Tagus River (38.7822 N, 9.0913 W). The dry Eisenia bicyclis seaweed was purchased in a commercial surface from the Seara brand (B# T20220405, expiration date April 2022), as previously described in [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…The same procedure was performed to obtain a dry mass of Fucus aqueous extract, using 10 g/L biomass of Fucus vesiculosus (38% g/g yield). The procedure for obtaining a dry mass of Aramé aqueous extract using 33 g/L biomass of Eisenia bicylis (66% g/g yield) was already reported in a previous publication from our group [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…This seaweed is used industrially to extract sodium alginate, and for consumption by the population of East Asia in soups and salads [ 11 , 12 ]. The main bioactive compounds reported for Aramé are phenolic compounds, and polysaccharides [ 13 , 14 ]. The most abundant phenolic compounds are phlorotannins (eckol and other phloroglucinol derivatives), which were reported to have various biological activities, namely anti-diabetic and antioxidant activities [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…Three of the most consumed edible seaweeds, Aramé, Fucus, and Nori, were matched for the composition and biological activities associated to the aqueous extracts of the seaweeds. Though there are various reports about Aramé [ 13 , 14 ], Fucus [ 22 , 23 , 24 ], and Nori [ 34 , 35 ], individually there are no studies comparing their composition and beneficial health effects.…”

Section: Introductionmentioning

confidence: 99%

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Edible Seaweeds Extracts: Characterization and Functional Properties for Health Conditions

et al. 2023

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Seaweeds are popular foods due to claimed beneficial health effects, but for many there is a lack of scientific evidence. In this study, extracts of the edible seaweeds Aramé, Nori, and Fucus are compared. Our approach intends to clarify similarities and differences in the health properties of these seaweeds, thus contributing to target potential applications for each. Additionally, although Aramé and Fucus seaweeds are highly explored, information on Nori composition and bioactivities is scarce. The aqueous extracts of the seaweeds were obtained by decoction, then fractionated and characterized according to their composition and biological activity. It was recognized that fractioning the extracts led to bioactivity reduction, suggesting a loss of bioactive compounds synergies. The Aramé extract showed the highest antioxidant activity and Nori exhibited the highest potential for acetylcholinesterase inhibition. The identification of the bioactive compounds in the extracts allowed to see that these contained a mixture of phloroglucinol polymers, and it was suggested that Nori’s effect on acetylcholinesterase inhibition may be associated with a smaller sized phlorotannins capable of entering the enzyme active site. Overall, these results suggest a promising potential for the use of these seaweed extracts, mainly Aramé and Nori, in health improvement and management of diseases, namely those associated to oxidative stress and neurodegeneration.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Edible Seaweeds Extracts: Characterization and Functional Properties for Health Conditions

et al. 2023

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Anti-Hypercholesterolemia Effects of Edible Seaweeds Extracts and Metabolomic Changes in Hep-G2 and Caco-2 Cell Lines

Pacheco¹,

Coelho²

2023

Preprint

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Hypercholesterolemia is a major risk for the development of cardiovascular diseases (CVDs), the main cause of mortality worldwide, and it is characterized by high levels of circulating cholesterol. The drugs currently available for hypercholesterolemia control have several side effects, so it is necessary to develop new effective and safer therapies. Seaweeds serve as sources of several bio-active compounds with claimed beneficial effects. Eisenia bicyclis (Aramé) and Porphyra tenera (Nori) are edible seaweeds which were previously recognized as rich in bioactive compounds. In the present study, we aim to evaluate the anti-hypercholesterolemia effect of these two seaweeds ex-tracts and their health potential. Both extracts, but more efficiently Aramé extract demonstrated to have liver 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) inhibitory activity and to reduce approximately 30 % cholesterol permeation through human Caco-2 cells simulating the intestinal lining, targets for hypercholesterolemia treatments. An untargeted metabolomic assay on human intestinal Caco-2 and liver Hep-G2 cells lines exposed to Aramé and Nori extracts revealed changes in the cell’s metabolism as indicators of the extract’s health beneficial effects. The metabolic pathways affected by exposure to both extracts were associated to lipid metabolism, such as phospholipids, and fatty acid metabolism, pathways of amino acid, cofactors and vitamins and cellular respiration metabolism. More profound for Aramé treated cells, but also seen for Nori ex-posed cells, the metabolites modifications were associated with the protection against CVDs and other diseases and, to the improvement of cells oxidative stress tolerance. The results obtained for the anti-hypercholesterolemia properties, in addition to the revelation of the positive impact on cells metabolism, are an important contribution for further evaluation of these seaweeds extracts as functional foods or for CVDs prevention.

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Coenzyme Q10-Loaded Albumin Nanoparticles Protect against Redox Imbalance and Inflammatory, Apoptotic, and Histopathological Alterations in Mercuric Chloride-Induced Hepatorenal Toxicity in Rats

Ramadan,

El Zaiat,

Habashy

et al. 2023

Biomedicines

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Exposure to mercuric chloride (HgCl2), either accidental or occupational, induces substantial liver and kidney damage. Coenzyme Q10 (CoQ10) is a natural antioxidant that also has anti-inflammatory and anti-apoptotic activities. Herein, our study aimed to investigate the possible protective effects of CoQ10 alone or loaded with albumin nanoparticles (CoQ10NPs) against HgCl2-induced hepatorenal toxicity in rats. Experimental animals received CoQ10 (10 mg/kg/oral) or CoQ10NPs (10 mg/kg/oral) and were injected intraperitoneally with HgCl2 (5 mg/kg; three times/week) for two weeks. The results indicated that CoQ10NP pretreatment caused a significant decrease in serum liver and kidney function markers. Moreover, lowered MDA and NO levels were associated with an increase in antioxidant enzyme activities (SOD, GPx, GR, and CAT), along with higher GSH contents, in both the liver and kidneys of intoxicated rats treated with CoQ10NPs. Moreover, HgCl2-intoxicated rats that received CoQ10NPs revealed a significant reduction in the hepatorenal levels of TNF-α, IL-1β, NF-κB, and TGF-β, as well as an increase in the hepatic level of the fibrotic marker (α-SMA). Notably, CoQ10NPs counteracted hepatorenal apoptosis by diminishing the levels of Bax and caspase-3 and boosting the level of Bcl-2. The hepatic and renal histopathological findings supported the abovementioned changes. In conclusion, these data suggest that CoQ10, alone or loaded with albumin nanoparticles, has great power in reversing the hepatic and renal tissue impairment induced by HgCl2 via the modulation of hepatorenal oxidative damage, inflammation, and apoptosis. Therefore, this study provides a valuable therapeutic agent (CoQ10NPs) for preventing and treating several HgCl2-induced hepatorenal disorders.

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Nanoformulation of Seaweed Eisenia bicyclis in Albumin Nanoparticles Targeting Cardiovascular Diseases: In Vitro and In Vivo Evaluation

Cited by 4 publications

References 84 publications

Edible Seaweeds Extracts: Characterization and Functional Properties for Health Conditions

Edible Seaweeds Extracts: Characterization and Functional Properties for Health Conditions

Anti-Hypercholesterolemia Effects of Edible Seaweeds Extracts and Metabolomic Changes in Hep-G2 and Caco-2 Cell Lines

Coenzyme Q10-Loaded Albumin Nanoparticles Protect against Redox Imbalance and Inflammatory, Apoptotic, and Histopathological Alterations in Mercuric Chloride-Induced Hepatorenal Toxicity in Rats

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