Birth of rhesus monkey infant after transfer of embryos derived from in-vitro matured oocytes: Short communication

Schramm, R. Dee; Paprocki, Ann Marie

doi:10.1093/humrep/15.11.2411

Cited by 26 publications

(17 citation statements)

References 36 publications

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“…This source of the COCs is distinct from those in previous reports using both nonhuman primates and women. Most prior studies evaluating IVM in nonhuman primate models used oocytes from large antral follicles of monkeys primed with FSH for 6-7 days [42,60,69,70]. Both human and macaque oocytes isolated from large antral follicle resume spontaneous meiosis [52,[71][72][73][74].…”

Section: Discussionmentioning

confidence: 99%

“…Oocytes were examined 16 h postinsemination for the presence of two PB and two pronuclei to confirm fertilization. Fertilized oocytes were transferred to four-well dishes (Nalge Nunc International Co., Naperville, IL) containing 500 ll HECM-9 (serum free) equilibrated at 378C in 6% CO 2 , 5% O 2 , and 89% N 2 and covered with tissue culture oil (SAGE) [42,43]. Embryos were cultured under these conditions for 48 h and thereafter in HECM-9 with 5% FBS.…”

Section: Semen Preparation and Ivf/icsimentioning

confidence: 99%

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Cumulus-Oocyte Complexes from Small Antral Follicles During the Early Follicular Phase of Menstrual Cycles in Rhesus Monkeys Yield Oocytes That Reinitiate Meiosis and Fertilize In Vitro1

Peluffo

Barrett

Stouffer

et al. 2010

Biology of Reproduction

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The stage at which follicle-enclosed cumulus-oocyte complexes achieve developmental competence in primates is unknown. Therefore, studies were designed to characterize the ability of oocytes in small antral follicles present during the menstrual cycle to spontaneously resume meiosis, fertilize, and support early embryo development. Ovaries were removed from adult rhesus monkeys (n = 12) during the early follicular phase (Days 3-4) of spontaneous cycles. Small antral follicles were divided into five groups according to their diameter; group I: <0.5 mm; group II: 0.5-0.99 mm; group III: 1.0-1.49 mm; group IV: 1.5-1.99 mm; and group V: 2.0-2.5 mm. The cumulus-oocyte complex from healthy small antral follicles (devoid of dark oocytes or granulosa cells) were extracted (n = 199) and cultured for 48 h under different conditions: in TALP (tyrode, albumin, lactate, pyruvate) medium alone, SAGE medium alone, or plus gonadotropins. At 48 h, oocyte meiotic status and diameter were measured after treatment of cumulus-oocyte complexes with hyaluronidase. Cumulus-oocyte complexes derived from follicles of 0.5- to 2-mm diameter contain oocytes that typically reinitiate meiosis in the absence or presence of gonadotropins and fertilize via in vitro fertilization or intracytoplasmic sperm injection. Moreover, the inseminated oocytes can reach the morula stage but arrest. Thus, the ability of these oocytes to complete maturation, as monitored from subsequent embryonic development after fertilization, is suboptimal. Further studies on primate IVM of oocytes from SAFs are warranted in order for them to be considered as an additional, novel source of gametes for fertility preservation in cancer patients.

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Section: Discussionmentioning

confidence: 99%

Section: Semen Preparation and Ivf/icsimentioning

confidence: 99%

Cumulus-Oocyte Complexes from Small Antral Follicles During the Early Follicular Phase of Menstrual Cycles in Rhesus Monkeys Yield Oocytes That Reinitiate Meiosis and Fertilize In Vitro1

Peluffo

Barrett

Stouffer

et al. 2010

Biology of Reproduction

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“…Together with IVF and cryopreservation, the ability to successfully perform oocyte IVM has great potential as a tool in ART for infertile women and for basic understanding of oocyte biology. Although normal offspring have been produced using IVM/IVF technology in humans (25)(26)(27) and in monkeys (28), IVM is far from being a reliable technique for the production of developmentally competent oocytes in primates, unlike in cattle (29). The regulation of oocyte maturation in primates is poorly understood, and at present the application of IVM technology to treatment of infertility relies on empirical protocols.…”

Section: Discussionmentioning

confidence: 99%

Effects of in vitro maturation and age on oocyte quality in the rhesus macaque Macaca mulatta

Nichols

Gierbolini

González‐Martínez

et al. 2010

Fertility and Sterility

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“…Mature oocytes from female rhesus macaques (Macaca mulatta), hormonally stimulated as previously described (Schramm and Bavister, 1996;Schramm and Paprocki, 2000), were collected laparoscopically into 37°C TL-HEPES (Bavister, 1995) medium containing 5% bovine calf serum (Hyclone Laboratories Inc., Logan, UT) and 10 IU/ml heparin (ElkinsSinn, Cherry Hill, NJ). Sperm capacitation and oocyte insemination were performed as previously described (Bavister et al, 1983;Boatman and Bavister, 1984).…”

Section: Collection Insemination and Culture Of Primate Oocytes Andmentioning

confidence: 99%

Imaging Mitochondrial Organization in Living Primate Oocytes and Embryos using Multiphoton Microscopy

et al. 2003

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We employed multiphoton laser scanning microscopy (MPLSM) to image changes in mitochondrial distribution in living rhesus monkey embryos. This method of imaging does not impair development; thus, the same specimen can be visualized multiple times at various developmental stages. Not only does this increase the amount of information that can be gathered on a single specimen but it permits the correlation of early events with subsequent development in the same specimen. Here we demonstrate the utility of MPLSM for determining changes in mitochondrial organization at various developmental stages and show that rhesus zygotes possess a distinct accumulation of mitochondria between the pronuclei prior to syngamy. We present evidence that suggests that this pronuclear accumulation may be positively correlated with development to the blastocyst stage-in the same embryo-thereby illustrating how MPLSM can be used to correlate cellular dynamics of primate oocytes and early embryos with their developmental potential. Understanding the relationship between mitochondrial distribution and the subsequent development of mammalian embryos, particularly primates, will increase our ability to improve embryo culture technologies, including those used for human assisted reproduction.

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Birth of rhesus monkey infant after transfer of embryos derived from in-vitro matured oocytes: Short communication

Cited by 26 publications

References 36 publications

Cumulus-Oocyte Complexes from Small Antral Follicles During the Early Follicular Phase of Menstrual Cycles in Rhesus Monkeys Yield Oocytes That Reinitiate Meiosis and Fertilize In Vitro1

Cumulus-Oocyte Complexes from Small Antral Follicles During the Early Follicular Phase of Menstrual Cycles in Rhesus Monkeys Yield Oocytes That Reinitiate Meiosis and Fertilize In Vitro1

Effects of in vitro maturation and age on oocyte quality in the rhesus macaque Macaca mulatta

Imaging Mitochondrial Organization in Living Primate Oocytes and Embryos using Multiphoton Microscopy

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