Effects of in vitro maturation and age on oocyte quality in the rhesus macaque Macaca mulatta

Nichols, Stephanie M.; Gierbolini, Lynette; González‐Martínez, Janis; Bavister, Barry D.

doi:10.1016/j.fertnstert.2008.12.141

Cited by 19 publications

(11 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aging is also correlated with an increase in the aneuploidy rate of oocytes (17)(18)(19)(20). Our study illustrates that the age of the female can perturb oocyte maturation, which is corroborated by the Jones study (20).…”

supporting

confidence: 85%

“…To study the effect of maternal aging on oocyte maturation, we examined two key components of morphologic nuclear maturation: the percentage undergoing germinal vesicle breakdown (GVBD) and the first polar body (PB1) extrusion, because it is much more difficult and complicated to examine cytoplasmic maturation (17). At 3 hours after maturation, the percentage of GVBD was only 45% in the aging oocytes, far lower than the 70% seen in the pubertal oocytes.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Age-related changes in the localization of DNA methyltransferases during meiotic maturation in mouse oocytes

Zhang

et al. 2011

Fertility and Sterility

View full text Add to dashboard Cite

supporting

confidence: 85%

mentioning

confidence: 99%

Age-related changes in the localization of DNA methyltransferases during meiotic maturation in mouse oocytes

Zhang

et al. 2011

Fertility and Sterility

View full text Add to dashboard Cite

“…With this in mind, a survey of the methods used for investigating errors in chromosome segregation was conducted to search for the most appropriate technique to apply to horse oocytes. Four main techniques were found in the scientific literature: 1) chromosomal spreads 5,18,19 , 2) fluorescent in situ hybridization (FISH) 4,6,17,20 , 3) centromere count on monastrol-collapsed spindles 13,14,21,22 , and 4) chromosome fluorescence staining without chromosome count 23,24,25 .…”

Section: Discussionmentioning

confidence: 99%

“…This procedure is called oocyte in vitro maturation (IVM) and represents a less drug-oriented, less expensive, and more patient-friendly approach to assisted reproductive technology. However, the success of embryo development with in vitro-matured oocytes is generally lower than with in vivo matured oocytes 4,5 . A possible explanation is that in vitro matured oocytes are more affected by errors in chromosome segregation, and the resulting aneuploidy impairs normal embryonic development…”

Section: Introductionmentioning

confidence: 99%

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Franciosi

Tessaro

Dalbiès-Tran

et al. 2017

JoVE

View full text Add to dashboard Cite

The field of assisted reproduction has been developed to treat infertility in women, companion animals, and endangered species. In the horse, assisted reproduction also allows for the production of embryos from high performers without interrupting their sports career and contributes to an increase in the number of foals from mares of high genetic value. The present manuscript describes the procedures used for collecting immature and mature oocytes from horse ovaries using ovum pick-up (OPU). These oocytes were then used to investigate the incidence of aneuploidy by adapting a protocol previously developed in mice. Specifically, the chromosomes and the centromeres of metaphase II (MII) oocytes were fluorescently labeled and counted on sequential focal plans after confocal laser microscope scanning. This analysis revealed a higher incidence in the aneuploidy rate when immature oocytes were collected from the follicles and matured in vitro compared to in vivo. Immunostaining for tubulin and the acetylated form of histone four at specific lysine residues also revealed differences in the morphology of the meiotic spindle and in the global pattern of histone acetylation. Finally, the expression of mRNAs coding for histone deacetylases (HDACs) and acetyl-transferases (HATs) was investigated by reverse transcription and quantitative-PCR (q-PCR). No differences in the relative expression of transcripts were observed between in vitro and in vivo matured oocytes. In agreement with a general silencing of the transcriptional activity during oocyte maturation, the analysis of the total transcript amount can only reveal mRNA stability or degradation. Therefore, these findings indicate that other translational and post-translational regulations might be affected.

show abstract

“…The observed 3% aneuploidy rate was greatly reduced compared with that of a previous study, suggesting that higher aneuploidy rates in embryos derived from donor oocytes could be due to aggressive hormonal stimulation. A similar study in the rhesus macaque suggested that in vitro maturation of oocytes can induce meiotic abnormalities in oocytes, especially those obtained from older females [Nichols et al, 2010].…”

Section: Fsh and Human Oogenesismentioning

confidence: 80%

Predisposition to Aneuploidy in the Oocyte

Vialard

Boitrelle²,

Molina-Gomès³

et al. 2011

Cytogenet Genome Res

View full text Add to dashboard Cite

While the incidence of predisposition to aneuploidy in the oocyte increases with age, there is also evidence of increased incidence in young women with recurrent miscarriage, recurrent aneuploidy, or recurrent implantation failure after in vitro fertilization. There is evidence from mouse models and from observations in humans that follicle-stimulating hormone (FSH) probably has a direct or indirect effect on the occurrence of oocyte aneuploidy. It seems that increased endogenous or exogenous FSH could induce meiotic disruption. Although the effect of FSH may explain the age-related increase in aneuploidy rate, many questions remain regarding young women, even if their FSH level is sometimes increased. Disruption of meiotic gene expression caused by exposure to environmental contaminants or by gene defects could also predispose to oocyte aneuploidy. Such abnormalities could impact on the oocyte pool, recombination and synapsis during fetal life, or oocyte growth.

show abstract

Effects of in vitro maturation and age on oocyte quality in the rhesus macaque Macaca mulatta

Cited by 19 publications

References 50 publications

Age-related changes in the localization of DNA methyltransferases during meiotic maturation in mouse oocytes

Age-related changes in the localization of DNA methyltransferases during meiotic maturation in mouse oocytes

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Predisposition to Aneuploidy in the Oocyte

Contact Info

Product

Resources

About