Cumulus-Oocyte Complexes from Small Antral Follicles During the Early Follicular Phase of Menstrual Cycles in Rhesus Monkeys Yield Oocytes That Reinitiate Meiosis and Fertilize In Vitro1

Peluffo, M; Barrett, Susan L.; Stouffer, Richard L.; Hennebold, Jon D.; Zelinski, Mary B.

doi:10.1095/biolreprod.110.084418

Cited by 27 publications

(28 citation statements)

References 77 publications

(103 reference statements)

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“…Similar observations have been made in other domestic animals, such as goats and new world primates (14,15). Apart from follicular diameter, oocyte diameter has also been correlated with the capacity to complete meiosis, acquire oocyte developmental capacity, and support embryo development in large mammals (16)(17)(18)(19), monkeys, and humans (20)(21)(22).…”

supporting

confidence: 66%

Developmental capacity of in vitro–matured human oocytes retrieved from polycystic ovary syndrome ovaries containing no follicles larger than 6 mm

Guzmán

Ortega-Hrepich

Albuz

et al. 2012

Fertility and Sterility

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supporting

confidence: 66%

Developmental capacity of in vitro–matured human oocytes retrieved from polycystic ovary syndrome ovaries containing no follicles larger than 6 mm

Guzmán

Ortega-Hrepich

Albuz

et al. 2012

Fertility and Sterility

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“…These data support the idea that maintaining the cumulus-oocyte connection is essential for oocyte cytoplasmic maturation. Furthermore, the relative smaller oocyte diameter in the 0L-COC group (;99.7 lm) indicated that these oocytes might originate from less developed follicles [40,41], and the lack of surrounding cumulus suggested that they might be from earlier atretic follicles [42]. Compared to the ML-COC group, there were fewer oocytes in the 1L-COC group that progressed to MII.…”

Section: Discussionmentioning

confidence: 93%

“…Compared to the ML-COC group, there were fewer oocytes in the 1L-COC group that progressed to MII. Given that the 1L-COC oocytes had similar size and the same proportion of normal spindle structure and chromosome alignment as the ML-COC ones, those that did mature should have a similar developmental capacity as ML-COC oocytes [41,43]. Unfortunately, the low number of MII-stage oocytes prevented the direct characterization of fertilization and embryo development of 1L-COC oocytes.…”

Section: Discussionmentioning

confidence: 99%

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In Vitro Oocyte Maturation and Preantral Follicle Culture from the Luteal-Phase Baboon Ovary Produce Mature Oocytes

Fazleabas

Shikanov

et al. 2010

Biology of Reproduction

106

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Female cancer patients who seek fertility preservation but cannot undergo ovarian stimulation and embryo preservation may consider 1) retrieval of immature oocytes followed by in vitro maturation (IVM) or 2) ovarian tissue cryopreservation followed by transplantation or in vitro follicle culture. Conventional IVM is carried out during the follicular phase of menstrual cycle. There is limited evidence demonstrating that immature oocyte retrieved during the luteal phase can mature in vitro and be fertilized to produce viable embryos. While in vitro follicle culture is successful in rodents, its application in nonhuman primates has made limited progress. The objective of this study was to investigate the competence of immature luteal-phase oocytes from baboon and to determine the effect of folliclestimulating hormone (FSH) on baboon preantral follicle culture and oocyte maturation in vitro. Oocytes from small antral follicle cumulus-oocyte complexes (COCs) with multiple cumulus layers (42%) were more likely to resume meiosis and progress to metaphase II (MII) than oocytes with a single layer of cumulus cells or less (23% vs. 3%, respectively). Twenty-four percent of mature oocytes were successfully fertilized by intracytoplasmic sperm injection, and 25% of these developed to morula-stage embryos. Preantral follicles were encapsulated in fibrin-alginate-matrigel matrices and cultured to small antral stage in an FSH-independent manner. FSH negatively impacted follicle health by disrupting the integrity of oocyte and cumulus cells contact. Follicles grown in the absence of FSH produced MII oocytes with normal spindle structure. In conclusion, baboon luteal-phase COCs and oocytes from cultured preantral follicles can be matured in vitro. Oocyte meiotic competence correlated positively with the number of cumulus cell layers.This study clarifies the parameters of the follicle culture system in nonhuman primates and provides foundational data for future clinical development as a fertility preservation option for women with cancer.

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“…The medulla region of the second ovary from each monkey was used for small antral follicle (diameter = 0.5-1.5 mm) isolation as described previously [15]. Isolated follicles were pooled by monkey (10 follicles/monkey) for RNA isolation as described above.…”

Section: Rna Sequencingmentioning

confidence: 99%

Anti-Müllerian hormone is produced heterogeneously in primate preantral follicles and is a potential biomarker for follicle growth and oocyte maturation in vitro

Letaw

et al. 2016

J Assist Reprod Genet

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Purpose The main goals of this study were to investigate the expression of anti-Müllerian hormone (AMH) and its receptor (AMHR2) during follicular development in primates, and to evaluate the potential of AMH as a biomarker for follicle growth and oocyte maturation in vitro. Methods The mRNA and protein expression of AMH and AMHR2 were determined using isolated follicles and ovarian sections from rhesus macaques (n = 4) by real-time PCR and immunohistochemistry, respectively. Isolated secondary follicles were cultured individually. Follicle growth and media AMH concentrations were assessed by ELISA. The mRNA expression profiles, obtained from RNA sequencing, of in vitro-and in vivo-developed antral follicles were compared. Secondary follicles from additional animals (n = 35) were cultured. Follicle growth, oocyte maturation, and media AMH concentrations were evaluated for forecasting follicular development in vitro by AMH levels. Results AMH immunostaining was heterogeneous in the population of preantral follicles that were also stained for AMHR2. The mRNA expression profiles were comparable between in vivo-and in vitro-developed follicles. AMH levels produced by growing follicles were higher than those of nongrowing follicles in culture. With a cutoff value of 1.40 ng/ml, 85 % of nongrowing follicles could be identified while eliminating only 5 % of growing follicles. Growing follicles that generated metaphase II-stage oocytes secreted greater amounts of AMH than did those yielding immature germinal vesicle-stage oocytes. Conclusions AMH, co-expressed with AMHR2, was produced heterogeneously by preantral follicles in macaques with levels correlated positively with follicle growth and oocyte maturation. AMH may serve as a biomarker for primate follicular development in vitro.

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Cumulus-Oocyte Complexes from Small Antral Follicles During the Early Follicular Phase of Menstrual Cycles in Rhesus Monkeys Yield Oocytes That Reinitiate Meiosis and Fertilize In Vitro1

Cited by 27 publications

References 77 publications

Developmental capacity of in vitro–matured human oocytes retrieved from polycystic ovary syndrome ovaries containing no follicles larger than 6 mm

Developmental capacity of in vitro–matured human oocytes retrieved from polycystic ovary syndrome ovaries containing no follicles larger than 6 mm

In Vitro Oocyte Maturation and Preantral Follicle Culture from the Luteal-Phase Baboon Ovary Produce Mature Oocytes

Anti-Müllerian hormone is produced heterogeneously in primate preantral follicles and is a potential biomarker for follicle growth and oocyte maturation in vitro

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