Biotin-Streptavidin Enzyme-linked Immunosorbent Assay for the Determination of Dibutyl Phthalate in Beverages and Drinking Water Using a Specific Polyclonal Antibody

Self Cite

Polybrominated diphenyl ethers are contaminants that are widely distributed in the environment and in food samples. In this study, a highly sensitive biotin-streptavidin system-based real-time immuno-polymerase chain reaction assay (BA-IPCR) was developed to detect 2,2´,4,4´-tetrabromodiphenyl ether (BDE-47). The BDE-47 immunogen and coating antigen were prepared, and a specific polyclonal anti-BDE-47 antibody was obtained. Several physiochemical factors in the immunoassay were optimised. The established BA-IPCR method was used to detect BDE-47 with a linearity ranging from 10 pg L −1 to 100 ng L −1 . The limit of detection was 2.96 pg L −1 . The recovery of the spiked samples was 92.97-107.19% and the coefficient of variation was 6.93-11.06%. The proposed method was applied to successfully detect BDE-47 in marine fish. The BA-IPCR data for the detection of BDE-47 were consistent with gas chromatography-mass spectrometry findings. The BA-IPCR method is accurate, sensitive and suitable for detecting trace amount of BDE-47 in food samples.

Section: Comparison With the Correlated Methodsmentioning

confidence: 99%

“…According to the relevant immunoassay (Sun & Zhuang, 2015), 0.5% gelatin was used as blocking reagent. Several other reaction conditions were optimised.…”

Section: Optimisation Of Reaction Conditionsmentioning

confidence: 99%

Biotin–streptavidin system-based real-time immuno-polymerase chain reaction for sensitive detection of 2,2′,4,4′-tetrabromodiphenyl ether in marine fish

Zhuang

2018

Self Cite

“…On the basis of above, the biotin-streptavidin system has introduced an enzyme-based immunoassay to amplify reaction signal because the biotin-streptavidin enzyme-linked immunoassays (BA-ELISA) has demonstrated that eight-folds higher sensitivity than the traditional ELISA under the same antibody and coating antigen (Wang, Zhang, Gao, Duan, & Wang, 2010). This strategy has been reported by the Zhuang group, including the biotin-streptavidin enzyme-based immunoassay for the determination of PAEs mainly in beverages and drinking water, milk and milk products and wine samples (Sun & Zhuang, 2014, 2015a, 2015b, 2015c.…”

Section: Biotin-streptavidin Enzyme-based Immunoassay Strategies For Pae Detectionmentioning

confidence: 99%

Development of immunoassays for the determination of phthalates

Zhang

2020

The development of highly selective analytical methodologies to monitoring phthalates (phthalic acid esters, PAEs) is important for the safety of environment, food and equipment. As one of the most important and classical interactions in biological systems, antigen-antibody interactions constitute a highly specific chemical nature in biological systems, which inspire us to develop highly specific, highly selective and high-throughput immunoassays for monitoring PAEs in different fields. We here attempt to give an introduction of the recent development particularly focusing on the immunoassay based on the specific binding of antigen-antibody for detecting PAEs. We also present an overview of the potential applications of the antibody labelling strategies to immunoassays for PAE investigations. Finally, we present the major challenges and opportunities regarding the high selectivity and specificity of PAE analysis based on the antigen-antibody interaction. We believe that this review provides critical insights into highly selective PAE analysis and gives an understanding of immunoassays.

“…Phthalic acid esters, as a class of plasticizers, are often added to plastic food-packaging materials to increase the plasticity and ductility of the plastic (Benjamin, Pradeep, Josh, Kumar, & Masai, 2015;Sørensen, 2006;Sun, & Zhuang, 2015a). However, such plasticizers tend to migrate into foods as a result of environmental factors such as storage temperature and food polarity (Keresztes, Tatar, Záray, & Czegeny, 2013;Salazar-Beltrán, Hinojosa-Reyes, Ruiz-Ruiz, Hernández-Ramírez, & Guzmán-Mar, 2018;Shi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Development of molecularly imprinted biomimetic immunoassay method based on quantum dot marker for detection of phthalates

Wang

Chen

et al. 2019