Biotin–streptavidin system-based real-time immuno-polymerase chain reaction for sensitive detection of 2,2′,4,4′-tetrabromodiphenyl ether in marine fish
Abstract:Polybrominated diphenyl ethers are contaminants that are widely distributed in the environment and in food samples. In this study, a highly sensitive biotin-streptavidin system-based real-time immuno-polymerase chain reaction assay (BA-IPCR) was developed to detect 2,2´,4,4´-tetrabromodiphenyl ether (BDE-47). The BDE-47 immunogen and coating antigen were prepared, and a specific polyclonal anti-BDE-47 antibody was obtained. Several physiochemical factors in the immunoassay were optimised. The established BA-IP… Show more
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