Biotechnological Uses in Assessment of Some Meat Products Adulteration with Equine Meat

Mousa, Mohamed M.; Nashwa, Nashwa; Helmy, Mohamed; Nasser, M. E. A.

doi:10.5455/ajvs.252393

Cited by 4 publications

(8 citation statements)

References 15 publications

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“…No cross-reaction, again inhibition or unexpected PCR products was detected in the blended tissues. samples were contaminated with donkey, goat and sheep meat, respectively, Moreover, 6%, 4% and 4% of sausage samples were contaminated with donkey, goat and sheep meat, respectively, in reverse to what was marked., Dn contrary, this was lower than that of Mousa et al (2017) who revealed that the incidence of adulteration in luncheon, hot dog, sausage and minced meat with horse meat were 24%, 8%, 64% and 56%, respectively in Alexandria city of Egypt.…”

Section: Application Of Species-specific Pcr Assay To Meat Productscontrasting

confidence: 62%

“…So there are numerous demonstrative techniques which are prepared by depending on protein and DNA investigation (Kesmen et al, 2007;Callao & Ruisanchez, 2018). Advance in DNA innovation has prompted quick development of alternative approaches for species identification (Mousa et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Adulteration with donkey and horse meat, in the past, had been reported by few specialists in Egypt (El-Shewy, 2007;Abd El-Nasser et al, 2010;Jaayid, 2013;Mousa et al, 2017). However contamination rate with donkey meat in our investigation is higher than that detailed in Assuit governorate in Egypt by Abd El-Nasser et al (2010) in minced meat (7%) and sausage (8%); this might be ascribed to our stress on the street vendors where there is a powerless control on food.…”

Section: Application Of Species-specific Pcr Assay To Meat Productsmentioning

confidence: 98%

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Species – specific PCR test for the quick recognition of equine tissue in raw and processed beef meat mixtures

et al. 2019

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PCR was applied for the discovery of adulteration of crude and processed beef meat with horse and donkey tissue. This was performed by blending (w/w) horse or donkey meat to beef meat in an extent up to 1:10000 (0.01%). The sensitivity was resolved as high as 0.01%. All used primers showed specificity in the PCR reactions utilizing layout DNAs from three animal species. PCR application on 96 beef meat and meat product samples gathered randomly from street vendors and prominent retail markets (24 of burger, 16 of minced meat, 24 of kofta, 16 of sausage, 7 of raw meat and 9 of launcheon) uncovered 6 positive for donkey tissue (3 from sausage, 2 from minced meat and 1 from kofta) and 2 positive for horse tissue (from sausage). This basic PCR strategy effectively distinguished adulteration of raw and processed beef meat samples with horse and donkey tissue. This work also highlights on the severity of the meat adulteration problem in Egypt.

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Section: Application Of Species-specific Pcr Assay To Meat Productscontrasting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Application Of Species-specific Pcr Assay To Meat Productsmentioning

confidence: 98%

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Species – specific PCR test for the quick recognition of equine tissue in raw and processed beef meat mixtures

et al. 2019

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“…Abd El-Razik et al [16] tested 96 meat samples and reported lower percentage of mislabelling (only two sausage samples were contaminated with horse meat). On the other hand, Mousa et al [18] revealed that the incidence of adulteration in 100 analysed meat products with horse meat was 38 % in Alexandria city of Egypt. Chisholm et al [8] performed horse presence assays on commercial samples including cured meats: salami and ham, but the study was carried out only with samples with declared horse content.…”

Section: Application Of Pcr Assay For Screening Of Meat Products With...mentioning

confidence: 99%

PCR analysis for meat products authenticity - detection of horse meat

Stefanova

Gotcheva²,

Angelov³

2023

BIO Web Conf.

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Food adulteration regarding species origin of meats is a common problem in the meat products sector. With regard to horse meat, its undeclared use in food products is not only a fraud, but could present a health risk since is often associated with the presence of the veterinary drug phenylbutazone in meat products. Therefore, it is important to use reliable methods for authentication of meat products regarding their species composition, which are applicable to complex food matrices. Polymerase chain reaction (PCR) with species-specific primers remains the most widely used analytical approach to detect species-related food adulteration due to its high sensitivity and specificity. The aim of the present study was to establish the authenticity of 20 different meat products on the Bulgarian market without declared horse meat content by using a species-specific PCR method. The specificity test of the PCR method used showed no amplification of DNA from beef and pork. A detection limit of 0.01% horse DNA in three-component meat mixtures was established for the PCR method. The PCR method enabled detection of undeclared presence of horse meat in 25% of the analyzed meat products, which demonstrates the need for strict control regarding authenticity in the meat food chain.

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“…Substitution to illegally used species is so difficult to identify by visual inspection after grinding and/or heat processing in such products (Abd El-Nasser et al, 2010). In the past, few specialists in Egypt had confirmed adulteration with donkey and horse meat (Mousa et al, 2017;Abd El-Razik et al, 2019).…”

Section: Specificity Of the Species-specific Primersmentioning

confidence: 99%

Bacteriological assessment and multiplex-PCR test for the detection of meat adulteration of different animal species

et al. 2021

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Biotechnological Uses in Assessment of Some Meat Products Adulteration with Equine Meat

Cited by 4 publications

References 15 publications

Species – specific PCR test for the quick recognition of equine tissue in raw and processed beef meat mixtures

Species – specific PCR test for the quick recognition of equine tissue in raw and processed beef meat mixtures

PCR analysis for meat products authenticity - detection of horse meat

Bacteriological assessment and multiplex-PCR test for the detection of meat adulteration of different animal species

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