Bacteriological assessment and multiplex-PCR test for the detection of meat adulteration of different animal species

Abuelnaga, Azza Sayed Mohamed; El-Razik, Khaled A. Abd; Ata, Nagwa S.; Hedia, Riham Hasaan; Elgabry, Elgabry Abd-Elalim; Marie, Hanan Shawky Abdel Wahab

doi:10.1590/fst.11520

Cited by 5 publications

(3 citation statements)

References 23 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to an investigation into outbreaks of collective foodborne disease (CBD) in Kenitra Provincial Hospital during the period (2007 -2009), they revealed 14 cases of CBD in the Gharb-Chrarda-Bnihsein region, with the largest number of outbreaks reported in Kenitra city with a percentage of 50%, including poultry meat which is among the foodstuffs responsible for CBD with a rate of 14% [4]. During slaughter and processing, all potentially edible tissues are subjected to contamination from a variety of sources within and outside the animal [5]. The pathogenic microorganisms that are implicated in contaminating meat and its products include; Salmonella spp., Shigella spp., Campylobacter jejuni, Campylobacter coli, Yersinia enterocolitica, verotoxigenic Escherichia coli and Listeria monocytogenes [6].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the microbiological and hygienic quality of turkey meat between six districts of the Kenitra city

et al. 2021

View full text Add to dashboard Cite

Meat is known to be one of the vehicles for many diseases to humans. The aim of this study is the comparison of the microbiological and hygienic quality of fresh turkey meat marketed in the most popular districts of Kenitra city. Fresh meat samples were taken from stores localized in six different districts. Microorganisms tests were conducted according to the appropriate standards. The Aerobic Mesophilic Flora (AFM) was most counted in district five (7.69±0.212 log10ufc/g), while the highest total and fecal coliform charges were obtained in district six with rates of 7.68±0.160 and 6.89±0.132 log10ufc/g, respectively. Cases of Salmonella spp were observed in all districts, except district five with frequencies up to 10.71%. Pseudomonas aeuroginosa charge was high in district five and its prevalence was high (21.42%) in districts two, four and six. Regarding Clostridium perfringens, the charges were up to 2.11±0.55 log10ufc/g in district six and a high prevalence of 42.85% was in district four. Escherichia coli showed dominance in all the districts studied with a high prevalence in district four with a rate of 75% and a high charge in district five (4.37 log10ufc/g). The presence of Staphylococcus aureus was significant in district four with a rate of 28.57% and a high concentration in district five (4.47 log10ufc/g). This study has shown great variability in the results found between the different districts and the rate of contaminations affecting this product. In fact, this microbiological and hygienic quality of raw turkey meat sold in these districts was judged marginal indicating the need for improved hygienic standards.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparison of the microbiological and hygienic quality of turkey meat between six districts of the Kenitra city

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Due to its simplicity, speed, and specificity, polymerase chain reaction (PCR) applications have been widely used ( Kesmen et al ., 2007 ; El-Demerdash et al ., 2023a ). According to several studies ( Abuelnaga et al ., 2020 ; Shalaby et al ., 2021 ; Megahed et al ., 2023 ), multiplex PCR is a powerful approach that can simultaneously amplify several templates, lower the detection rate, and get beyond the restrictions of a single PCR that only targets one type of meat at a time.…”

Section: Introductionmentioning

confidence: 99%

Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration

Sheikh,

Whab,

ElDaly

et al. 2024

Open Vet J

View full text Add to dashboard Cite

Background: Minced meat is a valuable source of nutrients, but it is vulnerable to contamination by microorganisms commonly present in the environment. Additionally, there is a risk of adulteration with cheaper meat sources, which can be harmful to consumers. Aim: It is crucial to identify meat adulteration with distinct microbiological analysis for legal, economic, religious, and public health purposes. Methods: A total of 100 minced meat samples were collected from several markets in Sharkia Governorate, Egypt. These samples were then subjected to bacteriological testing and an advanced multiplex PCR method. This method enables the detection of bovine, equine, porcine, and dog species in meat samples with just one step. Results: The adulterated samples had a higher total bacterial count and pH values compared to pure bovine meat. These differences in bacterial count and pH values were statistically significant, with p-values of 0.843 (log10) and 0.233, respectively. The frequency of E. coli occurrence was 13%, and the O111 serotype was predominant in the adulterated samples. Listeria monocytogenes and S. aureus were isolated with prevalence rates of 3% and 29%, respectively. Besides, the SYBR-green multiplex real-time PCR assay used in this study detected adulteration with dog, equine, and porcine meats in the examined samples at rates of 9%, 5%, and 4%, respectively. Conclusion: This method provides a sensitive and specific approach to detect issues related to well-being and safety.

show abstract

“…Meat is an important wellspring of different micronutrients, which are fundamental for development. It contains about 20 to 35% protein, all essential amino acids, fatty acids, minerals, and vitamins (Abuelnaga et al 2020). The highest produced meat categories in the world were bovine, poultry and pig (Fengou et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Multiplex PCR Assay for Simultaneous Detection of Beef Meat Fraud with Different Species

2023

Int J Vet Sci

View full text Add to dashboard Cite

Identification of species in animal-derived foods is a crucial component of its management. Legal, financial, religious, and public health considerations all apply to food adulteration, particularly in the case of beef meat. Food adulteration mostly includes replacement of low price ingredient in meat products to get unlawful higher benefits. To protect consumers from meat adulteration, various methods have been investigated. The DNA-based techniques are rapid, greater resistance to degradation, adequate for the detection of small amounts of DNA in complex handled food varieties. This work was designed to investigate different concentrations of beef meat adulterations with other meat types (equine, sheep, dog, and pork) using multiplex PCR. Fresh meat samples of different animal species cattle, donkey, pork and dog were collected for detection of their adulteration. Meat species samples were minced to make meat mixture for mimicking adulteration. The multiplex PCR assay for five meat species was run effectively, clarifying five unique PCR fragments. These PCR sections compared the particular sizes expected for the five designated species. The results showed successful amplification of the target cyt b gene sequences with the expected amplicon sizes (271pb) for cattle, (274pb) for sheep, and (808pb) for dog meat. Amplification of the target mt DNA, and 12S rRNA-tRNA Val gene sequences with the expected amplicon sizes (359pb) for equine, and (290bp) for pork's meat. The developed multiplex PCR assay was sensitive enough to detect 0.5% (w/w) adulterated meat under mixed matrices. It was concluded that the multiplex PCR could greatly minimize the cost for detection of meat adulteration.

show abstract

Bacteriological assessment and multiplex-PCR test for the detection of meat adulteration of different animal species

Abstract: Materials and methods Meat samplesRaw meat samples from 11 different animal species were collected randomly from Giza Governorate's butcher shops, common retail markets, veterinary faculties, Zoo and hospitals.

Cited by 5 publications

References 23 publications

Comparison of the microbiological and hygienic quality of turkey meat between six districts of the Kenitra city

Comparison of the microbiological and hygienic quality of turkey meat between six districts of the Kenitra city

Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration

Multiplex PCR Assay for Simultaneous Detection of Beef Meat Fraud with Different Species

Contact Info

Product

Resources

About