Biotechnological Requirements for the Commercial Cultivation of Macrofungi: Substrate and Casing Layer

Carrasco, Jaime; Tello, María Luisa Grau; Pérez, María Paz Sáez; Preston, Gail

doi:10.1007/978-3-030-02622-6_7

Cited by 10 publications

(14 citation statements)

References 24 publications

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“…This result was similar to those of previous studies which reported that a casing layer or casing soil overlaying the substrate was a necessary step in the primordia formation of P. portentosus [9,27,68]. Notably, a casing layer was a crucial step in the formation of a fruiting body and could effectively increase the productivity yield of some saprobic mushrooms, e.g., Agaricus bisporus, Agaricus subufescens, Coprinus comatus, Grifola frondosa and Stropharia rugosoannulata [70][71][72]. In this study, the primordia of P. spongiosus were successfully developed to mature fruiting bodies.…”

Section: Discussionsupporting

confidence: 91%

A New Report on Edible Tropical Bolete,Phlebopus spongiosusin Thailand and Its Fruiting Body Formation without the Need for a Host Plant

2020

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Phlebopus spongiosus is a well-known edible ectomycorrhizal mushroom indigenous to southern Vietnam. The mushroom specimens collected from northern Thailand in this study were identified as P. spongiosus. This identification was based on morphological characteristics and the multi-gene phylogenetic analyses. Pure cultures were isolated and the relevant suitable mycelial growth conditions were investigated. The results indicated that the fungal mycelia grew well on L-modified Melin-Norkans, and Murashige and Skoog agar all of which were adjusted to a pH of 5.0 at 30 C. Sclerotia-like structures were observed on cultures. The ability of this mushroom to produce fruiting bodies in the absence of a host plant was determined by employing a bag cultivation method. Fungal mycelia completely covered the cultivation substrate after 90-95 days following inoculation of mushroom spawn. Under the mushroom house conditions, the highest amount of primordial formation was observed after 10-15 days at a casing with soil:vermiculite (1:1, v/v). The primordia developed into a mature stage within one week. Moreover, identification of the cultivated fruiting bodies was confirmed by both morphological and molecular methods. This is the first record of P. spongiosus found in Thailand and its ability to form fruiting bodies without a host plant.

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Section: Discussionsupporting

confidence: 91%

A New Report on Edible Tropical Bolete,Phlebopus spongiosusin Thailand and Its Fruiting Body Formation without the Need for a Host Plant

2020

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“…Substrates designed for the commercial cultivation of Basidiomycetes are basically divided in two main groups: (i) fermented‐pasteurized substrates and (ii) a mixture of raw materials that have been steam sterilized (Carrasco et al ., ,b). Agaricus bisporus and Pleurotus ostreatus are two of the species cultivated worldwide on fermented substrates, in which the microbiome plays a significant role in fungal growth and fructification (Vajna et al ., ; Vieira and Pecchia, 2018).…”

Section: The Microbiome Associated With Soil Substrate and Casing Mamentioning

confidence: 99%

“…Agaricus bisporus and Pleurotus ostreatus are two of the species cultivated worldwide on fermented substrates, in which the microbiome plays a significant role in fungal growth and fructification (Vajna et al ., ; Vieira and Pecchia, 2018). Fermented substrates are produced through composting from agricultural waste such as wheat straw and chicken manure or horse manure as base materials (Carrasco et al ., ,b). Although this process, aeration has a significant impact on the compost microbiota, promoting synergistic and sequential enzymatic deconstruction of the lignocellulosic biomass (Antunes et al ., ).…”

Section: The Microbiome Associated With Soil Substrate and Casing Mamentioning

confidence: 99%

Growing edible mushrooms: a conversation between bacteria and fungi

Carrasco

Preston

2019

Environmental Microbiology

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Summary Mushroom cropping consists of the development and fructification of different fungal species in soil or selective substrates that provide nutrients and support for the crop. The microorganisms present in these environments strongly influence, and in some cases are required for the growth and fructification of cultivated mushrooms. Some fungi such as truffles and morels form ectomycorrhizal associations with host plants. For these fungi, helper bacteria play an important role in the establishment of plant‐fungal symbioses. Selective processes acting on the microbiota present in substrates and soils determine the composition of the microbiota inhabiting the fruit bodies or interacting with fungal hyphae, and both configure the mushroom holobiont, understood as the fungus plus associated microorganisms. Here, we review current knowledge regarding the cross‐talk between bacteria and fungi during mushroom cultivation. We highlight the potential use of bioinoculants as agronomical amendments to increase mushroom productivity through growth promotion or as biocontrol agents to control pests and diseases.

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“…2 ) (Sánchez 2010 ; Gaitán-Hernández et al 2014 ; Liang et al 2016 ; Pardo-Giménez et al 2016 , 2018 ; Xie et al 2017 ). Although many other varieties have been successfully cultivated (Carrasco et al 2018 ), some of them present commercial limitations due to the reduced yield achieved. Supplementation represents an important strategy to improve the yield and commercial viability of these less widely cultivated varieties.…”

Section: New Varieties Of Cultivated Mushrooms To Diversify the Indusmentioning

confidence: 99%

Supplementation in mushroom crops and its impact on yield and quality

et al. 2018

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Mushroom supplementation is an agronomic process which consists of the application of nutritional amendments to the substrates employed for mushroom cultivation. Different nitrogen and carbohydrate rich supplements have been evaluated in crops with a substantial impact on mushroom yield and quality; however, there is still controversy regarding the nutritional requirements of mushrooms and the necessity for the development of new commercial additives. The addition of external nutrients increases the productivity of some low-yielding mushroom varieties, and therefore is a useful tool for the industry to introduce new commercially viable varieties. Spent mushroom compost is a waste material that could feasibly be recycled as a substrate to support a new commercially viable crop cycle when amended with supplements. On the other hand, a new line of research based on the use of mushroom growth promoting microorganisms is rising above the horizon to supplement the native microbiota, which appears to cover nutritional deficiencies. Several supplements employed for the cultivated mushrooms and their agronomic potential in terms of yield and quality are reviewed in this paper as a useful guide to evaluate the nutritional requirements of the crop and to design new formulas for commercial supplementation.

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Biotechnological Requirements for the Commercial Cultivation of Macrofungi: Substrate and Casing Layer

Cited by 10 publications

References 24 publications

A New Report on Edible Tropical Bolete,Phlebopus spongiosusin Thailand and Its Fruiting Body Formation without the Need for a Host Plant

A New Report on Edible Tropical Bolete,Phlebopus spongiosusin Thailand and Its Fruiting Body Formation without the Need for a Host Plant

Growing edible mushrooms: a conversation between bacteria and fungi

Supplementation in mushroom crops and its impact on yield and quality

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