A New Report on Edible Tropical Bolete,<i>Phlebopus spongiosus</i>in Thailand and Its Fruiting Body Formation without the Need for a Host Plant

Kumla, Jaturong; Suwannarach, Nakarin; Lumyong, Saisamorn

doi:10.1080/12298093.2020.1784592

Cited by 7 publications

(15 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sanmee et al (2010) found that P. portentosus mycelia grew well on modified Gamborg, modified Melin Norkans and MS media at 30 o C and pH 4. Kumla et al (2020) found that P. spongiosus mycelia grew well on L-modified Melin-Norkans and Murashige and Skoog agar at 30 o C at an initial pH of 5. Siri-in et al (2014) studied the growth conditions of pure Scleroderma sinnamariense mycelia.…”

Section: Discussionmentioning

confidence: 98%

Untitled

2023

JJBS

View full text Add to dashboard Cite

Study areas include cell biology, genomics, microbiology, immunology, molecular biology, biochemistry, embryology, immunogenetics, cell and tissue culture, molecular ecology, genetic engineering and biological engineering, bioremediation and biodegradation, bioinformatics, biotechnology regulations, gene therapy, organismal biology, microbial and environmental biotechnology, marine sciences. The JJBS welcomes the submission of manuscript that meets the general criteria of significance and academic excellence. All articles published in JJBS are peerreviewed. Papers will be published approximately one to two months after acceptance.

show abstract

Section: Discussionmentioning

confidence: 98%

Untitled

2023

JJBS

View full text Add to dashboard Cite

show abstract

Section: Molecular Identificationmentioning

confidence: 98%

Cultivation of Edible Ectomycorrhizal Mushrooms (Phelobopus portentosus) Associated with Sesbania javanica Miq

2023

JJBS

View full text Add to dashboard Cite

Phlebopus portentosus is a popular wild edible mushroom that is found growing on both forest trees and fruit trees. Because it can grow either with various trees or without a host tree, it is reported that it is not a mycorrhizal fungus and may not be an obligate ectomycorrhizae. Furthermore, it was recognized that P. portentosus can be found in association with leguminous plants, such as Sesbania javanica Miq. The aims of this study were to cultivate P. portentosus with bioinoculants in solid and liquid forms and track the ectomycorrhizal association with Sesbania javanica Miq. A pure mycelium culture of this fungus was isolated, and the growth parameters, temperature, medium pH, and types of medium were examined. The mycelium grew very well on synthetic media such as MPDA and YM. The optimum pH and temperature were pH 4 and 30 o C, respectively. Bioinoculants of P. portentosus were inoculated on seedbeds of Sesbania javanica Miq. After inoculation, ectomycorrhizal-like roots were found at 6 months, and sporocarps were found at 8.5 months. To prove this, the ITS rDNA sequences were aligned, and the results revealed that all sequences of the ectomycorrhizal-like root tips and sporocarps that developed on seedbeds of S. javanica Miq. and black bolete used as a bioinoculant were 100% identical to those of P. portentosus MN962534 (GenBank database). This finding indicates that P. portentosus can form ectomycorrhizae with S. javanica Miq.

show abstract

“…Finally, a gold coating was applied before observations were conducted using the JSM IT 200 SEM system manufactured by JEOL in Tokyo, Japan. Morphological identification was based on several references, including Boedjin (1951), Kumla et al (2012), Pham et al (2012), Kumla et al (2020), and Baroni et al (2015).…”

Section: Morphological Identificationmentioning

confidence: 99%

“…eaten mushroom in China and Thailand due to its high nutrient content and popularity in the local market (Ji et al 2011;Kumla et al 2012;Kumla et al 2020). In addition, this species holds the distinction of being the initial species within the Boletaceae order to undergo large-scale industrial cultivation (Ji et al 2011;Kumla et al 2015).…”

mentioning

confidence: 99%

The Unpopular Edible Bolete (Phlebopus portentosus) in Indonesia

Putra,

Nurhayat,

Sibero

et al. 2024

HAYATI J Biosci

View full text Add to dashboard Cite

Phlebopus portentosus (Berk. & Broome) Boedijn was firstly constructed from the collection of Indonesia in 1951. To date, the subsequent collection of this ectomycorrhizal (ECM) fungi has not been done in Indonesia. In addition, the utilization information of this edible mushroom as food is not popular for the country. The goal of our work was to update the current collection, provide the morphological and molecular data, and promote the utilization of this edible mushroom in Indonesia. Fresh fruiting bodies were evaluated for morphological and molecular evidence. The basidiomata were analysis on the basis of the morphological and molecular evidence. The phylogenetic tree was constructed following the rDNA-ITS 1/2 sequence. P. portentosus was verified by morphological and phylogenetic studies combined. The indigenous people in the research site use this wild edible mushroom for self-consumption and sell it to local market. Phlebopus portentosus BO24626 was solitary to connate, boletoid basidioma, yellowish to greenish brown pileus and stipe, yellowish hymenophore, clavate stipe, club shaped basidia, oval to subglobose basidiospores, cystidia present. The absence of sponge-like tissues and the occurrence of sterigmata distinguished our specimens from the similar morphological species P. spongious. The lack of reddish stipe distinguished our specimen from P. roseus. In addition, the presence of hymenial cystidia delimits our specimens from P. colossus. The inferred phylogenetic tree nested our specimen in the group of P. portentosus (sister to P. spongiosus). The ITS sequence of our specimen is now deposited at GenBank and can be applied to upcoming research of P. portentosus.

show abstract

A New Report on Edible Tropical Bolete,Phlebopus spongiosusin Thailand and Its Fruiting Body Formation without the Need for a Host Plant

Cited by 7 publications

References 55 publications

Untitled

Untitled

Cultivation of Edible Ectomycorrhizal Mushrooms (Phelobopus portentosus) Associated with Sesbania javanica Miq

The Unpopular Edible Bolete (Phlebopus portentosus) in Indonesia

Contact Info

Product

Resources

About