Biosynthesized Nanosilver from Alginate Dialdehyde: An In Vitro Evaluation

Abstract

The superior antimicrobial properties of nanosilver (AgNP) led to its applications in healthcare and hygiene products. Herein, we report an efficient and simple green chemistry approach for the synthesis of AgNP by in‐situ reduction and capping by an eco‐friendly carbohydrate polymer, alginate dialdehyde (ADA). The UV‐Vis absorption maxima of the ADA‐capped silver nanoparticles (ADA‐AgNPs) showed a surface plasmon resonance peak at 411 nm, a peak characteristic to silver nanoparticles, within 30 min of the onset of the reaction. They were spherical, had an average diameter of 18.68±2.5 nm and had face‐centered cubic structure. Its storage stability was tested up to 90 days by monitoring its zeta potential and it was found to be stable around −35.1±1.71 mV. ADA‐AgNPs exhibited good antimicrobial properties against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) and excellent antibiofilm activity against S. aureus at its minimum inhibitory concentration of 34 μg/mL. ADA‐AgNPs showed good anticancer action against HCT‐116 cells too.

Neuronal cell response on aligned fibroporous electrospun mat generated from silver ion complexed ethylene vinyl alcohol copolymer

“…These peak shifts were presumably due to the complexation of Ag + with –OH groups of EVAL (Figure 3B). 25–27 It is reported that in Ag + to Ag 0 reduction during nanoparticle formation, the peaks for Ag3d 3/2 and Ag3d 5/2 were observed at 374.3 and 368.3 eV respectively and represents the presence of a metallic silver 28,29 . So, these results confirmed the complexation of Ag+ with the –OH groups of EVAL (Scheme 1) and no metallic silver is present.…”

“…[25][26][27] It is reported that in Ag + to Ag 0 reduction during nanoparticle formation, the peaks for Ag3d 3/2 and Ag3d 5/2 were observed at 374.3 and 368.3 eV respectively and represents the presence of a metallic silver. 28,29 So, these results confirmed the complexation of Ag+ with the -OH groups of EVAL (Scheme 1) and no metallic silver is present. presence of such shoulder peaks also indicates that the silver is in Ag + state instead of Ag 0 .…”

“…The presence of such shoulder peaks also indicates that the silver is in Ag + state instead of Ag 0 25 . The silver ions underwent reduction and the presence of nanoparticles usually gave peaks around 400 nm 28,29 . So, the absence of such prominent peaks around 400 nm also suggests the non‐occurrence of Ag + reduction to Ag 0 (Figure 3C) and absence of nanopaticles.…”