Biosynthesis of Lycorine by<i>in Vitro</i>Cultures of<i>Pancratium Maritimum</i>L. (Amaryllidaceae)

Bogdanova, Y.; Pandova, B.; Yanev, S.; Stanilova, Marina

doi:10.1080/13102818.2009.10818572

Cited by 17 publications

(8 citation statements)

References 10 publications

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“…The Scientific World Journal maritimum [2] and Sternbergia species [14] as well as tissue culture of Pancratium maritimum [15], Narcissus confusus [10], and Leucojum aestivum [16].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples ofHymenocallis littoralisby HPLC-UV Method

Subramaniam

Sundarasekar

Sahgal

et al. 2014

The Scientific World Journal

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The Hymenocallis littoralis, an ornamental and medicinal plant, had been traditionally used for wound healing. In the present study, an analytical method using HPLC with ultraviolet detection was developed for the quantification of lycorine in the extracts of different parts of wild plant and tissue culture samples of H. littoralis. The separation was achieved using a reversed-phase column. The method was found to be accurate, repeatable, and sensitive for the quantification of minute amount of lycorine present in the samples. The highest lycorine content was found in the bulb extract (2.54 ± 0.02 μg/mg) whereas the least was in the root extract (0.71 ± 0.02 μg/mg) of the wild plants. Few callus culture samples had high content of lycorine, comparable to that of wild plants. The results showed that plant growth regulators, 2,4-dichlorophenoxyacetic acid (2,4-D) alone at 4.5 μM (2.58 ± 0.38 μg/mg) or a combination of 2,4-D at 9.00 μM with 4.5 μM of 6-benzylaminopurine (BAP), were the optimum concentrations for the production of high lycorine (2.45 ± 0.15 μg/mg) content in callus culture. The present analytical method could be of value for routine quantification of lycorine in the tissue culture production and standardization of the raw material or extracts of H. littoralis.

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“…The Scientific World Journal maritimum [2] and Sternbergia species [14] as well as tissue culture of Pancratium maritimum [15], Narcissus confusus [10], and Leucojum aestivum [16].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples ofHymenocallis littoralisby HPLC-UV Method

Subramaniam

Sundarasekar

Sahgal

et al. 2014

The Scientific World Journal

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“…The development of in vitro techniques for screening and producing of these alkaloids is important technological step by the mean to prevent the natural populations lost as well as to ensure the continuous supply of these substances to pharmacy. Up to now, there are scanty data concerning the alkaloid production by P. maritimum L. in vitro cultures (4,5) and such data for in vitro production of compounds with acetylcholinesterase inhibitory action by P. maritimum L. were not found in accessible scientific literature.…”

Section: Introductionmentioning

confidence: 98%

“…Sea daffodil attracts great interest by researchers because of the produced alkaloids, some of which possessing valuable pharmaceutical properties (3,4).…”

Section: Introductionmentioning

confidence: 99%

Obtaining and Selection ofPancratium MaritimumL.In VitroCultures with Acetylcholinesterase Inhibitory Action

Georgiev

Ivanov

Pavlov

2010

Biotechnology & Biotechnological Equipment

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Two types of plant in vitro systems -calli and shoot cultures were obtained from ovaries of sea daffodil (Pancratium maritimum L.) -a Bulgarian threatened plant belonging to the Amaryllidaceae family. Using TLC method, ten different alkaloids were separated in extracts from in vitro cultures, as well as their abilities to inhibit the acetylcholinesterase (EC. 3.1.1.7) were evaluated by the same techniques. All investigated lines from both type in vitro systems exhibited high levels of somaclonal variability concerning the number and the levels of alkaloids produced. However, the callus cultures produced alkaloids in significantly lower levels compared to the shoot cultures. Two shoot lines (Н6L7 and Н6L6) producing four alkaloids with high acetylcholinesterase inhibitory action (with Rf values of 0.47, 0.43, 0.25 and 0.12) they were selected as prospective source for further screening of new acetylcholinesterase inhibitors.

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“…Plant in vitro systems have been extensively used for continuous, sustainable, and economically viable production of bioactive plant secondary metabolites [ 22 ]. A significant success has been demonstrated in the development of in vitro systems for production of Amaryllidaceae alkaloids [ 23 , 24 , 25 , 26 , 27 , 28 ]. Optimization of the production process is also well documented, starting with nutrient media optimization [ 29 ], development of appropriate bioreactor design [ 24 , 30 , 31 ], and optimization of the cultivation conditions [ 27 , 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Recent Progress in Amaryllidaceae Biotechnology

2020

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Plants belonging to the monocotyledonous Amaryllidaceae family include about 1100 species divided among 75 genera. They are well known as medicinal and ornamental plants, producing pharmaceutically important alkaloids, the most intensively investigated of which are galanthamine and lycorine. Amaryllidaceae alkaloids possess various biological activities, the most important one being their anti-acetylcholinesterase activity, used for the treatment of Alzheimer’s disease. Due to increased demand for Amaryllidaceae alkaloids (mainly galanthamine) and the limited availability of plant sources, in vitro culture technology has attracted the attention of researchers as a prospective alternative for their sustainable production. Plant in vitro systems have been extensively used for continuous, sustainable, and economically viable production of bioactive plant secondary metabolites. Over the past two decades, a significant success has been demonstrated in the development of in vitro systems synthesizing Amaryllidaceae alkaloids. The present review discusses the state of the art of in vitro Amaryllidaceae alkaloids production, summarizing recently documented plant in vitro systems producing them, as well as the authors’ point of view on the development of biotechnological production processes with a focus on the future prospects of in vitro culture technology for the commercial production of these valuable alkaloids.

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Biosynthesis of Lycorine byin VitroCultures ofPancratium MaritimumL. (Amaryllidaceae)

Cited by 17 publications

References 10 publications

Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples ofHymenocallis littoralisby HPLC-UV Method

Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples ofHymenocallis littoralisby HPLC-UV Method

Obtaining and Selection ofPancratium MaritimumL.In VitroCultures with Acetylcholinesterase Inhibitory Action

Recent Progress in Amaryllidaceae Biotechnology

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