Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples of<i>Hymenocallis littoralis</i>by HPLC-UV Method

Subramaniam, Sreeramanan; Sundarasekar, Jeevandran; Sahgal, Geethaa; Murugaiyah, Vikneswaran

doi:10.1155/2014/408306

Cited by 12 publications

(9 citation statements)

References 18 publications

(30 reference statements)

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“…from 0 to 7 µg/g [ 22 , 38 , 39 ], and higher concentrations from 10 to 13 µg/g DW were reported for L. aestivum [ 36 , 44 ]. Similar results were obtained for the biosynthesis of lycorine from undifferentiated callus cultures [ 38 , 39 , 41 , 44 ]. These low yields of the target alkaloids indicate that undifferentiated tissue cultures are inappropriate for developing successful and highly productive biotechnology for their production.…”

Section: Biotechnological Production Of Amaryllidaceae Alkaloidssupporting

confidence: 83%

Recent Progress in Amaryllidaceae Biotechnology

2020

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Plants belonging to the monocotyledonous Amaryllidaceae family include about 1100 species divided among 75 genera. They are well known as medicinal and ornamental plants, producing pharmaceutically important alkaloids, the most intensively investigated of which are galanthamine and lycorine. Amaryllidaceae alkaloids possess various biological activities, the most important one being their anti-acetylcholinesterase activity, used for the treatment of Alzheimer’s disease. Due to increased demand for Amaryllidaceae alkaloids (mainly galanthamine) and the limited availability of plant sources, in vitro culture technology has attracted the attention of researchers as a prospective alternative for their sustainable production. Plant in vitro systems have been extensively used for continuous, sustainable, and economically viable production of bioactive plant secondary metabolites. Over the past two decades, a significant success has been demonstrated in the development of in vitro systems synthesizing Amaryllidaceae alkaloids. The present review discusses the state of the art of in vitro Amaryllidaceae alkaloids production, summarizing recently documented plant in vitro systems producing them, as well as the authors’ point of view on the development of biotechnological production processes with a focus on the future prospects of in vitro culture technology for the commercial production of these valuable alkaloids.

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Section: Biotechnological Production Of Amaryllidaceae Alkaloidssupporting

confidence: 83%

Recent Progress in Amaryllidaceae Biotechnology

2020

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“…This may be due to the effect of plant growth regulators (PGRs), such as auxin and cytokinins used in this report. It is known that PGRs have effects not only on cell differentiation and proliferation but also on the pathway of cell biosynthesis . For instance, 2,4‐D induces callus formation when it is used in combination with auxins because it promotes DNA synthesis and mitosis…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, our work followed the conditions described previously, 9,13 Table 2. Internal standard (IS): codeine; IAA, indole acetic acid; BAP, 6-benzylaminopurine; 2,4-D, 2,4-dichlorophenoxyacetic SCHEME 1 Biosynthetic relationships between the different alkaloid types identified in Rhodophiala pratensis 18 For instance, 2,4-D induces callus formation when it is used in combination with auxins because it promotes DNA synthesis and mitosis. 19 The research shows that the method used allows a good number of bulbs to be obtained quickly and inclusion of different combinations of growth regulators may significantly influence the alkaloid biosynthesis which makes it a suitable route for alkaloid production.…”

Section: Alkaloid Profilesmentioning

confidence: 99%

In vitro micropropagation and alkaloids analysis by GC–MS of Chilean Amaryllidaceae plants: Rhodophiala pratensis

Trujillo-Chacón

Pastene

Bustamante

et al. 2019

Phytochemical Analysis

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Introduction Plants from Amaryllidaceae family are of interest since they produce a particular type of alkaloid useful for the treatment of neurodegenerative diseases of the central nervous system, such as Galanthamine. Given the low content of these secondary metabolites in the plant, it is necessary to study mechanisms to increase the productivity of them. Objective To obtain fast qualitative and quantitative analysis of the alkaloids and extend the understanding of biosynthesis and metabolism in these kinds of plants. Furthermore, establish a reliable, simple and fast analytical method for the in vitro callus culture of vegetative organs for Rhodophiala pratensis species. Methods The alkaloids composition of the callus culture of R. pratensis were analysed by gas chromatography coupled with mass spectrometry (GC–MS). Results A methodology for the qualitative and quantitative analysis of the alkaloids present in fresh callus culture of this wild plant species was established. The analysis showed alternation in the alkaloids type ratio and number of compounds between wild bulbs, in vitro bulbs and callus. It was possible to identify 24 alkaloids from a pool of 60 signals whose fragmentation pattern corresponds to the alkaloids of Amaryllidaceae plants. Together with the aforementioned, the amount and type of alkaloid present in the plant material obtained by in vitro culture of R. pratensis was determined in the same way. The results show the high biosynthetic potential of in vitro grown bulbs and callus tissue that are able to produce significant amounts of pharmacologically relevant alkaloids from R. pratensis in various proportions that depend on the culture conditions such as supplementation with growth substances. The in vitro grown bulbs produce an alkaloidal extract that contain a 52.6% w/w of alkaloids. Conclusion This study allowed the alkaloid content in callus culture of R. pratensis to be found by means of GC–MS. These results allowed a relationship between the type of growth regulator and the type of alkaloids found to be established. Finally, we can say that the results achieved to state that the production of alkaloids using different combinations of growth regulators could be directed during in vitro micropropagation from provided plant material.

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“…These effects of lycorine in A375 cells might be regulated through the inhibition of the PI3K signaling pathway. As a natural alkaloid, lycorine exists in the flowers and bulbs of some Amaryllidaceae species, including spider lilies (Lycoris), daffodils (Narcissus) and snowdrops (Galanthus) [14]. Although the potential targets or underlying mechanisms of lycorine action are still unclear, lycorine is a promising potential clinical drug or lead for tumor treatment because of its various biological effects and low cytotoxicity [15,16].…”

Section: Discussionmentioning

confidence: 99%

Lycorine inhibits melanoma A375 cell growth and metastasis through the inactivation of the PI3K/AKT signaling pathway

Jiang¹,

Liu²

2018

Med Sci (Paris)

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Malignant melanoma, one of the most aggressive skin cancers, has a very high mortality rate. Currently, the number of drugs to treat melanoma is low. Although new immunotherapeutic approaches based on the use of antibodies against immune checkpoints have shown long term responses, it is urgent to develop novel anti-melanoma drugs with a high efficiency and a low toxicity in a large number of patients. Lycorine, a natural product, has been reported to exert antitumor effects on some cancers. However, the impact of lycorine on melanoma cells is still unknown. Using the CCK8 assay, we found that lycorine can suppress the proliferation of melanoma A375 cells in a dose-time-dependent manner. Moreover, a transwell assay showed that lycorine inhibited the migration and invasion of A375 cells significantly. Further, lycorine treatment could induce the apoptosis of the A375 cells. Biochemical analyses showed that the expression level of the anti-apoptosis Bcl-2 protein decreased, while the expression of the pro-apoptosis protein Bax and active caspase-3 increased after lycorine treatment. Finally, using western blot assay, we found that the antitumor effects of lycorine on A375 cells might be through the inactivation of the PI3K/Akt signaling pathway. Based on these observations, we suggest that lycorine may be an interesting candidate for further studies on its ability to represent a novel antitumor drug for human melanoma treatment in the future.

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Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples ofHymenocallis littoralisby HPLC-UV Method

Cited by 12 publications

References 18 publications

Recent Progress in Amaryllidaceae Biotechnology

Recent Progress in Amaryllidaceae Biotechnology

In vitro micropropagation and alkaloids analysis by GC–MS of Chilean Amaryllidaceae plants: Rhodophiala pratensis

Lycorine inhibits melanoma A375 cell growth and metastasis through the inactivation of the PI3K/AKT signaling pathway

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