Biosynthesis of Fe nanoparticles using Alhagi sparsifolia extract for the treatment of colorectal carcinoma in the in vitro condition: A pre-clinical trial study

Abstract

IntroductionThe preparation and formulation of new chemotherapeutic supplements and drugs with remarkable effects for the treatment of cancer are in the priority of both developing and developed countries. Recently, iron nanoparticles have been used as modern chemotherapeutic drugs for the treatment of several cancers such as leukemia, lung cancer, breast cancer, prostate cancer, etc. In the present study, iron nanoparticles were green-synthesized using the aqueous extract of Alhagi sparsifolia leaf aqueous extract.Material and methodsThe synthesized FeNPs were characterized by analytical techniques including SEM, TEM, UV-Vis., and FT-IR. The anti-human colorectal carcinoma activity of FeNPs was evaluated using MTT assay. In the cellular and molecular part of the recent study, the treated cells with FeNPs were assessed by MTT assay for 48h about the cytotoxicity and anti-human colorectal carcinoma properties on normal (HUVEC) and colorectal carcinoma cell lines i.e. HT-29, HCT 116, HCT-8, and Ramos.2G6.4C10.ResultsThe nanoparticles were formed in a spherical shape in the average size of 47.24 nm. In the antioxidant test, the IC50 of FeNPs and BHT against DPPH free radicals were 161 and 134 µg/mL, respectively. The viability of malignant colorectal cell line reduced dose-dependently in the presence of FeNPs. The IC50 of FeNPs were 250, 293, 276, and 344 µg/mL against HT-29, HCT 116, HCT-8, and Ramos.2G6.4C10 cell lines, respectively.ConclusionsAfter clinical study, iron nanoparticles containing Alhagi sparsifolia leaf aqueous extract may be used to formulate a new chemotherapeutic drug or supplement to treat the several types of human colorectal carcinoma.

One‐step synthesis of silver nanostructures using Heracleum persicum fruit extract, their cytotoxic activity, anti‐cancer and anti‐oxidant activities

“…A huge number of proofs showed that ROS was firmly identified with coronary illness, carcinogenesis, atherosclerosis, and different infections related to aging. Nowadays, manufactured cancer prevention agents, generally show undesirable secondary effects [28][29][30][31][32].…”

“…The progressive development of nanotechnology has been viewed as for regarding disease as well as to the large assortment of utilizations, particularly for medicine conveyance, symptomatic and imaging instances. There are numerous sorts' kinds of nanostructures accessible while picking the right transporters is a crucial matter [31,[41][42][43][44]. Interesting physicochemical properties of AgNPs gives them the simplicity of entry into the cells and enables them to effectively communicate with biomolecules inside the cells or on the cell surfaces [41][42][43][44][45].…”