Bio-inspired techniques are used for synthesis in terms of application, green chemical research, facile, and eco-friendly chemistry study of silver nanostructures utilizing plant extracts as natural reducing/stabilization and solid adjuvants without the use of toxic and damaging reagents. The present study investigates the biosynthesis of Ag nanoparticles via the mediation of the methanolic extract of Heracleum persicum seeds, without utilizing any stabilizer or surfactant. These nanostructures were identified utilizing ultravioletvisible spectroscopy, field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, high-resolution transmission electron microscopy, Fouriertransform infrared spectroscopy and dynamic light scattering. The attributes of AgNPs versus usual human breast adenocarcinoma cell lines that is, Hs 281.T, MDA-MB-468, AU565 [AU-565], MCF7, CAMA-1, SK-BR-3, NMU, and RBA were evaluated. The livability of breast adenocarcinoma cell line diminishes dose-dependently in the existence of AgNPs. After clinical studies, AgNPs can be used as a green drug in the treatment of human breast adenocarcinoma.
Heracleum persicum Desf. ex Fischer (Umbelliferae) is a herbaceous perennial plant distributed in Iran and Turkey. The aromatic fruits of this plant are commonly used as food additive, carminative, antiseptic and tonic. The present study was designed to isolate non-volatile constituents of H. persicum fruits and evaluate their anti-tumor potentials against different cancer cells. Phytochemical analysis using chromatography on silica gel and Sephadex LH-20 columns resulted in the isolation of phellopterin (1), angelicin (2), pimpinellin (3), bergapten (4), isopimpinellin (5) and xanthotoxin (6) from dichloromethane fraction along with apterin (7), isorhamnetin 3-O-glucoside (8), isorhamnetin 3-O-rutinoside (narcissin) (9), and quercetin 3-O-rutinoside (rutin) from n-butanol fraction of H. persicum fruits extract. 1H-NMR and 13C-NMR spectral analyses were applied to characterize the chemical structures. In MTT assay all of the tested compounds demonstrated preferential cytotoxic activity against cancer cells (IC50; 40–370 µg/mL) in comparison with HUVEC normal cells (IC50; > 1000 µg/mL). Among the compounds phellopterin (1) showed the highest anti-tumor activity toward U-266, SK-MM-1 and RPMI-8226 multiple myeloma cells with IC50 values of 44.3 ± 1.4, 69.1 ± 1.2 and 85.7 ± 1.8 µg/mL, respectively.
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