Biosynthesis, characterization and cytotoxicity of zinc nanoparticles using panax ginseng roots, araliaceae

Abstract

This study aims to biosynthesize zinc nanoparticles from the aqueous extract of Panax ginseng (Red Ginseng) roots. The characteristics of ZnNPs were checked using change in color, UV-Vis, SEM, SPM, AFM, FT-IR, and EDS analyses, and assessed their cytotoxicity against L20B tumor cell line using MTT assay. The change in the solution color after 3 hr on 70 °C is from yellow to the brownish color with whitish sediments. The adsorption peak of UV-vis is 340 nm as evidence of formation the Zn nanoparticles. AFM, SEM and EDS observe shapes of zinc nanoparticles which are spherical to irregular particles with rate of size 59.76 nm. The concentration 100% Zinc nanoparticles significantly (p<0.01) recorded best inhibition percentage 41.70% against murine fibroblast cells (L20B) which have receptors of human polioviruses. Thus, this work is considered as an auspicious first test to reduce the growth of cancers using green ZnNPs of Ginseng (Panax ginseng) in vitro.

Metallic Nanoparticles Obtained via “Green” Synthesis as a Platform for Biosensor Construction

“…Such advantages make metallic NPs obtained by green synthesis (gNPs) prospective for applications in biological analysis, drug delivery and imaging, environmental monitoring, industrial catalysis and electronic devices [11][12][13][14][15][16][17][18][19]. A special role of these gNPs is related to antimicrobial and antibiofilm agents [19][20][21][22][23][24][25].…”

“…Many plants [11,12,21,[24][25][26], algae [4], microorganisms [1,3,[7][8][9][10]14,18,23,[27][28][29][30], as well as redox-imbalanced mammalian cells and systems [31] are known to produce nanostructured mineral crystals and metallic NPs with properties similar to chemically-synthesized materials. Biological agents, including polysaccharides, polypeptides, DNA, enzymes secreted by cells [24,[32][33][34][35][36][37][38][39][40][41] and purified enzymes [42,43] are able to reduce noble metal ions to gain metallic NPs. Besides the practical aim of gNPs production, investigations on the green synthesis processes will be useful for elucidating the mechanisms of cells' responses to stress caused by metal ions.…”

“…The exact mechanism of gNP synthesis using biological agents has not been elucidated to date. However, it has been suggested that various biomolecules are involved in this process [24,[38][39][40][41][42][43][44][45][46][47][48]. Capping and stabilizing agents (polysaccharides, polypeptides and other bioorganic compounds) prevent the gNPs from further growth and agglomeration in resulted colloidal solution [45][46][47][48][49][50][51].…”

A comprehensive and systemic review of ginseng‐based nanomaterials: Synthesis, targeted delivery, and biomedical applications

“…The heating temperature at 70°C is optimal to increase activation energy and reduce organic molecules for ZnNPs synthesis. 6 2.4 | Silica nanoparticles (SiNPs)…”

Bio-Synthesized Nanoparticles in Developing Plant Abiotic Stress Resilience: A New Boon for Sustainable Approach

“…Furthermore, the biomedical applications of root-mediated synthesized NPs have also been documented in the literature. For instance, ZnNPs and AgNPs synthesized from the root extract of Panax ginseng [43] and Glycyrrhiza glabra [44] showed a significant role in the diagnosis of diseases, particularly cancer and gastric ulcers, respectively.…”