Metallic Nanoparticles Obtained via “Green” Synthesis as a Platform for Biosensor Construction

Gayda, Galina; Demkiv, Olha; Stasyuk, Nataliya; Serkiz, R.; Lootsik, Maxim D.; Errachid, Abdelhamid; Gonchar, Mykhailo; Nisnevitch, Marina

doi:10.3390/app9040720

Cited by 46 publications

(20 citation statements)

References 75 publications

(115 reference statements)

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“…Linearity was obtained in concentration range between 0.03 and 0.25 mM BP. The sensitivity was determined to be 649 A/Mxm 2 (Figure 4B), which corresponds to (or exceeds) the highest values described in the literature for amperometric analogues (Portaccio et al, 2013; Gayda et al, 2019). For example, the sensor, based on laccase from Trametes vesicolor , immobilized on the surface of graphite electrode, revealed the sensitivity of 232 A/Mxm 2 (Gayda et al, 2019).…”

Section: Resultssupporting

confidence: 55%

Extracellular laccase from Monilinia fructicola: isolation, primary characterization and application

Demkiv

Gayda

Broda

et al. 2020

Cell Biology International

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Laccases are enzymes belonging to the family of blue copper oxidases. Due to their broad substrate specificity, they are widely used in many industrial processes and environmental bioremediations for removal of a large number of pollutants. During last decades, laccases attracted scientific interest also as highly promising enzymes to be used in bioanalytics. The aim of this study is to obtain a highly purified laccase from an efficient fungal producer and to demonstrate the applicability of this enzyme for analytics and bioremediation. To select the best microbial source of laccase, a screening of fungal strains was carried out and the fungus Monilinia fructicola was chosen as a producer of an extracellular enzyme. Optimal cultivation conditions for the highest yield of laccase were established; the enzyme was purified by a column chromatography and partially characterized. Molecular mass of the laccase subunit was determined to be near 35 kDa; the optimal pH ranges for the highest activity and stability are 4.5–5.0 and 3.0–5.0, respectively; the optimal temperature for laccase activity is 30°C. Laccase preparation was successfully used as a biocatalyst in the amperometric biosensor for bisphenol A assay and in the bioreactor for bioremediation of some xenobiotics.

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Section: Resultssupporting

confidence: 55%

Extracellular laccase from Monilinia fructicola: isolation, primary characterization and application

Demkiv

Gayda

Broda

et al. 2020

Cell Biology International

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“…Green synthesis of nanoparticles has emerged as a novel approach in the field of nanotechnology due to its ability to provide effective control over growth and stabilization of nanocrystals [41]. Several research studies have used the green approach for synthesizing AgNPs in order to address the problem of environmental pollutants and harmful microorganisms.…”

Section: Optimization Of Parameters For Biosynthesis Of Agnpsmentioning

confidence: 99%

Green synthesis of silver nanoparticles using cauliflower waste and their multifaceted applications in photocatalytic degradation of methylene blue dye and Hg2+ biosensing

Kadam¹,

et al. 2020

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Green synthesis of silver nanoparticles (AgNPs) using plant extracts has emerged as a viable environment-friendly method. The aim of the study was to biosynthesize AgNPs using cauliflower (Brassica oleracea var. botrytis) waste extract and further test their potential applications in photocatalytic degradation of methylene blue (MB) dye and Hg 2+ biosensing. Optimum extract concentration, AgNO 3 concentration, pH and temperature required for biosynthesis of stable AgNPs were determined by UV-visible spectroscopy. FT-IR, XRD, SEM, TEM, SAED, XPS and BET analysis were performed for characterizing AgNPs. MB dye degradation using AgNPs was determined by analyzing the intensity of dye absorption maxima at 664 nm. Specificity and sensitivity of biosynthesized AgNPs for Hg 2+ ions were studied for assessing their biosensing abilities. Optimum conditions needed for biosynthesis of stable AgNPs were observed to be 3 ml extract, 0.5 mM AgNO 3 , pH 8.5 and microwave-assisted heating at 600 W for 5 min. FT-IR analysis showed that the extract contained necessary functional groups that facilitated biosynthesis of AgNPs. XRD, SEM, TEM, SAED, XPS results confirmed the formation of AgNPs. BET analysis showed that AgNPs had an average size of 35.08 nm and surface area of 19.22 m 2 /g. Maximum MB dye degradation percentage of 97.57% was obtained at 150 min without any significant silver leaching thereby, signifying notable photocatalytic property of AgNPs. Biosensing studies showed that AgNPs were specifically able to detect up to 0.1 mg/l Hg 2+ ions. In summary, cauliflower waste served as a useful source of reducing agents for biosynthesizing AgNPs with promising environmental applications.

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“…They have been reported to work either independently or in combination. Through oxidation-reduction reactions (REDOX), these enzymes catalyze the biotransformation of numerous compounds (Figure 8) [60,61,62].…”

Section: Oxidase/peroxidase Based-biosensorsmentioning

confidence: 99%

Enzyme-Based Electrochemical Biosensors for Microfluidic Platforms to Detect Pharmaceutical Residues in Wastewater

et al. 2019

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Emerging water pollutants such as pharmaceutical contaminants are suspected to induce adverse effects to human health. These molecules became worrisome due to their increasingly high concentrations in surface waters. Despite this alarming situation, available data about actual concentrations in the environment is rather scarce, as it is not commonly monitored or regulated. This is aggravated even further by the absence of portable and reliable methods for their determination in the field. A promising way to tackle these issues is the use of enzyme-based and miniaturized biosensors for their electrochemical detection. Here, we present an overview of the latest developments in amperometric microfluidic biosensors that include, modeling and multiphysics simulation, design, manufacture, testing, and operation methods. Different types of biosensors are described, highlighting those based on oxidases/peroxidases and the integration with microfluidic platforms. Finally, issues regarding the stability of the biosensors and the enzyme molecules are discussed, as well as the most relevant approaches to address these obstacles.

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Metallic Nanoparticles Obtained via “Green” Synthesis as a Platform for Biosensor Construction

Cited by 46 publications

References 75 publications

Extracellular laccase from Monilinia fructicola: isolation, primary characterization and application

Extracellular laccase from Monilinia fructicola: isolation, primary characterization and application

Green synthesis of silver nanoparticles using cauliflower waste and their multifaceted applications in photocatalytic degradation of methylene blue dye and Hg2+ biosensing

Enzyme-Based Electrochemical Biosensors for Microfluidic Platforms to Detect Pharmaceutical Residues in Wastewater

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