Biosensors based on piezoelectric crystals

Näbauer, A.; Berg, Patricia E.; Ruge, Ingolf; Müller, Elisabeth; Woias, Peter; Kößlinger, Conrad; Drobe, H.

doi:10.1016/0925-4005(90)80261-w

Cited by 23 publications

(6 citation statements)

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“…Inspired by the finding that the vibration of the quartz is considerably influenced by a liquid, the so-called reference crystal method has been developed [35]. With this setup, two quartz crystals are excited in parallel.…”

Section: Reference Crystal Methodsmentioning

confidence: 99%

“…Aberl and co-workers used diluted HSA as a medium to minimize nonspecific adsorption [103,105]. More general procedures to prevent this problem, which do not require the knowledge of the solution's composition, are the reference crystal method [35] and the same condition method [136]. A new procedure was introduced by Zhang et al [137], who were able to distinguish the mass load of the quartz from viscoelastic effects by measuring the resonance frequency and the amplitude of the applied voltage simultaneously.…”

Section: Piezoimmunosensorsmentioning

confidence: 99%

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Quartz Crystal Microbalance for Bioanalytical Applications

Janshoff

Steinem

2001

Sensors Update

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The quartz crystal microbalance (QCM) was first introduced as a mass sensor in gas phase and in vacuum. Since oscillator circuits capable of exciting shear vibrations of quartz resonators under liquid load have been developed, the QCM became accepted as a new powerful technique to monitor adsorption processes at solid/liquid interfaces in chemical and biological research rendering the method an attractive low‐cost alternative for bioanalytic applications. In the last decade, adsorption of biomolecules on functionalized surfaces turned out to be one of the paramount applications of piezoelectric transducers comprising the interaction of DNA and RNA with complementary strands, specific recognition of protein‐ligands by immobilized receptors, the detection of virus capsids, bacteria, mammalian cells and last but not least the development of complete immunosensors. Piezoelectric transducers allow a label‐free detection of molecules; they are more than mere mass sensors since the sensor response is also influenced by interfacial phenomena, viscoelastic properties of the adhered biomaterial, surface charges of adsorbed molecules and surface roughness. These new insights have recently been used to investigate the adhesion of cells, liposomes and proteins onto surfaces allowing to determine morphological changes of cells as a response to pharmacological substances and changes in the water content of biopolymers in situ. However, future will show whether the quartz crystal microbalance will assert itself against established label‐free sensor devices like surface plasmon resonance spectroscopy and interferometry.

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Section: Reference Crystal Methodsmentioning

confidence: 99%

Section: Piezoimmunosensorsmentioning

confidence: 99%

Quartz Crystal Microbalance for Bioanalytical Applications

Janshoff

Steinem

2001

Sensors Update

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show abstract

“…[143] In this way Göpel and co-workers succeeded in the arranged linkage of an antigenÐa synthetic peptide of the mouth and claw epidemic virusÐon a w-hydroxyundecanethiol monolayer. [124,126] More general procedures to prevent this problem, which do not require the knowledge of the solutions composition, are the reference crystal method [147] and the same-condition method. The functionalized surface is often blocked with a protein such as BSA or casein before the binding of the analyte so as to minimize nonspecific adsorption.…”

Section: Reviewsmentioning

confidence: 99%