Quartz Crystal Microbalance for Bioanalytical Applications

Janshoff, Andreas; Steinem, Claudia

doi:10.1002/chin.200317289

Cited by 8 publications

(11 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This structure resembles a PH-domain, which is the typical binding motif in PIP 2 -binding proteins. The QCM technique has been proven to be a versatile tool to monitor protein-lipid interactions in a label-free and timeresolved manner (21,31). Here, it allowed us to determine the protein concentrations required for maximum surface coverage, which were, independent of the protein, in the 100-nM range.…”

Section: F-actin Binding Of Ezrin Mutants and Ezrin Wtmentioning

confidence: 99%

Activation of F-Actin Binding Capacity of Ezrin: Synergism of PIP2 Interaction and Phosphorylation

Bosk

Braunger

Gerke

et al. 2011

Biophysical Journal

View full text Add to dashboard Cite

Ezrin is a membrane-cytoskeleton linker protein that can bind F-actin in its active conformation. Several means of regulation of ezrin's activity have been described including phosphorylation of Thr-567 and binding of L-α-phosphatidylinositol-4,5-bisphosphate (PIP(2)). However, the relative contributions of these events toward activation of the protein and their potential interdependence are not known. We developed an assay based on solid-supported membranes, to which different ezrin mutants (ezrin T567A (inactive mutant), wild-type, and T567D (active pseudophosphorylated mutant)) were bound, that enabled us to analyze the influence of phosphorylation and PIP(2) binding on ezrin's activation state in vitro. The lipid bilayers employed contained either DOGS-NTA-Ni to bind the proteins via an N-terminal His-tag, or PIP(2), to which ezrin binds via specific binding sites located in the N-terminal region of the protein. Quantitative analysis of the binding behavior of all three proteins to the two different receptor lipids revealed that all three bind with high affinity and specificity to the two receptor lipids. Fluorescence microscopy on ezrin-decorated solid-supported membranes showed that, dependent on the mode of binding and the phosphorylation state, ezrin is capable of binding actin filaments. A clear synergism between phosphorylation and the receptor lipid PIP(2) was observed, suggesting a conformational switch from the dormant to the active, F-actin binding state by recognition of PIP(2), which is enhanced by the phosphorylation.

show abstract

Section: F-actin Binding Of Ezrin Mutants and Ezrin Wtmentioning

confidence: 99%

Activation of F-Actin Binding Capacity of Ezrin: Synergism of PIP2 Interaction and Phosphorylation

Bosk

Braunger

Gerke

et al. 2011

Biophysical Journal

View full text Add to dashboard Cite

show abstract

“…The application of an external electrical potential to a piezoelectric material, such as quartz, produces internal mechanical stresses that induce an oscillating electric field which, in turn, initiates an acoustic wave throughout the crystal. These waves travel in a direction perpendicular to the plate surfaces (Ebato et al, 1994;Janshoff and Steinem, 2001;Mecea, 2005). Sauerbrey (1959) first described the relationship between observed frequency decrease ( f) and deposited mass (m) on the crystal surface in air or a vacuum (Mecea, 2005;Sauerbrey, 1959;Vaughan et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…The frequency changes observed when a liquid is passed over the QCM crystal surface are also dependent on both the density and viscosity of the solution (Kanazawa and Gordon, 1985). QCM devices are relatively simple and convenient to use and can detect rapid, real-time responses to binding events on the crystal surface, such as antigen-antibody interactions (Lee and Chang, 2005;Park et al, 2003;Skládal et al, 2004;Uttenthaler et al, 1998), and have been applied to several areas in biotechnology including clinical diagnosis (Janshoff and Steinem, 2001;Nath and Chilkoti, 2002) and environmental monitoring (Kurosawa et al, 2006). More recently, a new approach has been described to improve the sensitivity of QCM biosensors by the use of antibodyfunctionalized nanoparticles as a mass amplification probe for a QCM sensor, resulting in significant enhancement of sensitivity (Chu et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

The detection of influenza A and B viruses in clinical specimens using a quartz crystal microbalance

Hewa¹,

Tannock

Mainwaring³

et al. 2009

Journal of Virological Methods

View full text Add to dashboard Cite

Current methods for the accurate diagnosis of influenza based on culture of the virus or PCR are highly sensitive and specific but require specialised laboratory facilities and highly trained personnel and, in the case of viral culture, can take up to 14 days to obtain a definitive result. In this study, a quartz crystal microbalance-based immunosensor (QCM) has been developed and its potential evaluated for the rapid and sensitive detection of both influenza A and B viruses in laboratory-cultured preparations and clinical samples. The effective limit for detection by QCM for stock preparations of both A/PR/8/34 and B/Lee/40 viruses was 1 x 10(4) pfu/mL, associated with observed frequency shifts of 30 (+/-5) and 37 (+/-6.5) Hz, respectively. Conjugation of 13 nm gold nanoparticles to the detecting antibody improved the mass sensitivity of the immunosensor, resulting in a 10-fold increase in sensitivity and a detection limit of 1 x 10(3) pfu/mL for both preparations, with resulting frequency shifts of 102 (+/-11) and 115 (+/-5) Hz, respectively. Detection of virus in nasal washes with this technique was achieved by overnight passage in MDCK cultures prior to analysis. A comparison of results obtained from 67 clinical samples using existing RT-PCR, shell vial, cell culture and ELISA methods showed that QCM techniques were comparable in sensitivity and specificity to cell culture methods.

show abstract

“…Quartz crystal resonators (QCR) are adopted as acoustic load sensors exploiting the piezoelectric properties of quartz crystals. Typical application is the employment as quartz crystal microbalance (QCM) for measurement in biochemical applications where the mass of substances to be detected can change the vibrating mass of the resonator and hence its resonance frequency (Janshoff and Steinem. 2001).…”

Section: Introductionmentioning

confidence: 99%

Design, Simulation And Testing Of Planar Spiral Coils For The Time-Gated Interrogation Of Quartz Resonator Sensors

Farran

Baù²,

Modotto³

et al. 2014

ECMS 2014 Proceedings Edited By: Flaminio Squazzoni, Fabio Baronio, Claudia Archetti, Marco Castellani

View full text Add to dashboard Cite

A technique for contactless electromagnetic interrogation of quartz resonator sensors is proposed and validated. The technique is based on the separation in time of the excitation and detection phases, exploiting the sensing of the transient response of the resonator. Contactless operation is achieved by means of electromagnetic coupling between spiral planar coils connected to the interrogation circuit and the sensor. The typical operating frequencies of quartz resonators in the megahertz range limit the working range to short distances. Analytical modeling and numerical and finite element simulation of the geometry of the coils have allowed to extend working operating distances up to 10 cm.

show abstract

Quartz Crystal Microbalance for Bioanalytical Applications

Abstract: For Abstract see ChemInform Abstract in Full Text.

Cited by 8 publications

References 66 publications

Activation of F-Actin Binding Capacity of Ezrin: Synergism of PIP2 Interaction and Phosphorylation

Activation of F-Actin Binding Capacity of Ezrin: Synergism of PIP2 Interaction and Phosphorylation

The detection of influenza A and B viruses in clinical specimens using a quartz crystal microbalance

Design, Simulation And Testing Of Planar Spiral Coils For The Time-Gated Interrogation Of Quartz Resonator Sensors

Contact Info

Product

Resources

About